Development of a recombinant reporter Getah virus for antiviral drug screening assays

Ren, Tongwei; Zhou, Lingshan; Min, Xiangling; Sui, Mengqi; Zhi, Xuechun; Mo, Yongfang; Huang, Jing; Zhang, Kang; Liu, Wenbo; Wang, Hao; Wang, Xindong; Ouyang, Kang; Chen, Ying; Huang, Weijian; Wei, Zuzhang

doi:10.1016/j.vetmic.2023.109742

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…Upon comparing the replication of rBPIV3-SX-EGFP with wild-type parental BPIV3, our results demonstrated that rBPIV3-SX-EGFP exhibited similar growth kinetics to the parental virus. Additionally, the utilization of virus expression reporter genes serves as a powerful tool for screening and testing antiviral drugs (23)(24)(25). Ribavirin is a purine nucleoside derivative with broad-spectrum antiviral activity.…”

Section: Discussionmentioning

confidence: 99%

Construction and characterization of a reverse genetics system of bovine parainfluenza virus type 3c as a tool for rapid screening of antivirals in vitro

Han,

Lu,

Zhang

et al. 2024

Front. Vet. Sci.

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Bovine parainfluenza virus type 3 (BPIV3) is a key pathogen associated with bovine respiratory disease complex (BRDC). However, its specific pathogenesis mechanisms have not been fully elucidated. Reverse genetics provides a useful method for understanding the pathogenic mechanism of BPIV3. To ensure the functionality of the rescue platforms, we first constructed a minigenome (MG) system of BPIV3 utilizing a 5-plasmid system in this investigation. Then, a full-length infection clone of BPIV3 was obtained from the SX-2021 strain, and different methods were employed to identify the rescued virus. Additionally, we recovered a recombinant BPIV3 using the reverse genetics system that could express enhanced green fluorescence protein (eGFP). Through the growth curve assays, the replicate capability of rBPIV3-SX-EGFP was found to be similar to that of the parental virus. Subsequently, the rBPIV3-SX-EGFP was used to determine the antiviral activity of ribavirin. The results showed that ribavirin had an anti-BPIV3 effect in MDBK cells. In conclusion, the successful development of a reverse genetic system for the SX-2021 strain establishes a foundation for future studies on BPIV3, including investigations into its pathogenic mechanism, gene function, and antiviral screening properties.

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Section: Discussionmentioning

confidence: 99%

Construction and characterization of a reverse genetics system of bovine parainfluenza virus type 3c as a tool for rapid screening of antivirals in vitro

Han,

Lu,

Zhang

et al. 2024

Front. Vet. Sci.

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“…Since then, GETV has reportedly outbreak worldwide, such as East Eurasia and South-East Asian countries [ 2 ]. GETV could be amplified through the mosquito–vertebrate host–mosquito cycle and infected horses, pigs and cattle [ 3 ]. GETV infected swine of all ages, especially, newborn piglets, caused high morbidity, mortality, and serious economic losses [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

GETV nsP2 plays a critical role in the interferon antagonism and viral pathogenesis

Mou,

Meng,

Shi

et al. 2023

Cell Commun Signal

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Getah virus (GETV) was becoming more serious and posing a potential threat to animal safety and public health. Currently, there is limited comprehension regarding the pathogenesis and immune evasion mechanisms employed by GETV. Our study reveals that GETV infection exhibits the capacity for interferon antagonism. Specifically, the nonstructural protein nsP2 of GETV plays a crucial role in evading the host immune response. GETV nsP2 effectively inhibits the induction of IFN-β by blocking the phosphorylation and nuclear translocation of IRF3. Additionally, GETV nsP2 hinders the phosphorylation of STAT1 and its nuclear accumulation, leading to significantly impaired JAK-STAT signaling. Furthermore, the amino acids K648 and R649, situated in the C-terminal region of GETV nsP2, play a crucial role in facilitating nuclear localization. Not only do they affect the interference of nsP2 with the innate immune response, but they also exert an influence on the pathogenicity of GETV in mice. In summary, our study reveals novel mechanisms by which GETV evades the immune system, thereby offering a foundation for comprehending the pathogenic nature of GETV.

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A recombinant Getah Virus expressing a GFP gene for rapid neutralization testing and antiviral drug screening assay

Ren,

Liu,

Zhou

et al. 2024

Virology

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Development of a recombinant reporter Getah virus for antiviral drug screening assays

Cited by 3 publications

References 43 publications

Construction and characterization of a reverse genetics system of bovine parainfluenza virus type 3c as a tool for rapid screening of antivirals in vitro

Construction and characterization of a reverse genetics system of bovine parainfluenza virus type 3c as a tool for rapid screening of antivirals in vitro

GETV nsP2 plays a critical role in the interferon antagonism and viral pathogenesis

A recombinant Getah Virus expressing a GFP gene for rapid neutralization testing and antiviral drug screening assay

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