Development of a recombinant protein-based ELISA for detection of antibodies against bovine foamy virus

Materniak-Kornas, Magdalena; Osiński, Zbigniew; Rudzki, Marcin; Kuźmak, Jacek

doi:10.1515/jvetres-2017-0034

Cited by 13 publications

(18 citation statements)

References 20 publications

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“…A slightly lower rate of 39% was observed in Great Britain [127] and Australia [128]. The most recent data come from Germany, where only 7% of tested animals were identified as being BFV positive [15] and from Poland with BFV sero-prevalence of over 30% in dairy cattle [129]. BFV prevalence based on these studies seems to be very diverse.…”

Section: Bfv Epidemiology and Naturally Occurring Co-infectionsmentioning

confidence: 91%

Bovine Foamy Virus: Shared and Unique Molecular Features In Vitro and In Vivo

Materniak-Kornas

Tan

Heit-Mondrzyk

et al. 2019

Viruses

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The retroviral subfamily of Spumaretrovirinae consists of five genera of foamy (spuma) viruses (FVs) that are endemic in some mammalian hosts. Closely related species may be susceptible to the same or highly related FVs. FVs are not known to induce overt disease and thus do not pose medical problems to humans and livestock or companion animals. A robust lab animal model is not available or is a lab animal a natural host of a FV. Due to this, research is limited and often focused on the simian FVs with their well-established zoonotic potential. The authors of this review and their groups have conducted several studies on bovine FV (BFV) in the past with the intention of (i) exploring the risk of zoonotic infection via beef and raw cattle products, (ii) studying a co-factorial role of BFV in different cattle diseases with unclear etiology, (iii) exploring unique features of FV molecular biology and replication strategies in non-simian FVs, and (iv) conducting animal studies and functional virology in BFV-infected calves as a model for corresponding studies in primates or small lab animals. These studies gained new insights into FV-host interactions, mechanisms of gene expression, and transcriptional regulation, including miRNA biology, host-directed restriction of FV replication, spread and distribution in the infected animal, and at the population level. The current review attempts to summarize these findings in BFV and tries to connect them to findings from other FVs.

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Section: Bfv Epidemiology and Naturally Occurring Co-infectionsmentioning

confidence: 91%

Bovine Foamy Virus: Shared and Unique Molecular Features In Vitro and In Vivo

Materniak-Kornas

Tan

Heit-Mondrzyk

et al. 2019

Viruses

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“…While infections of humans by FVs from different simians and non-human primates are well evidenced, little is presently known about the possibility of such inter-species transmission caused by FVs of live-stock animals. Since BFV is highly prevalent within cattle populations [3,7,20], special attention should be paid to the possible involvement of BFV in inter-species transmission, especially regarding free-ranging wild ruminants. This is a very important and pertinent issue, owing to increasing human impact on the environment, globalization, and the establishment of breeding of some wild ruminants posing new threats including the uncontrolled transmission of infectious agents into wildlife [21,22].…”

Section: Introductionmentioning

confidence: 99%

Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?

Materniak-Kornas

Löchelt

Rola

et al. 2020

Viruses

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Foamy viruses (FVs) are widely distributed and infect many animal species including non-human primates, horses, cattle, and cats. Several reports also suggest that other species can be FV hosts. Since most of such studies involved livestock or companion animals, we aimed to test blood samples from wild ruminants for the presence of FV-specific antibodies and, subsequently, genetic material. Out of 269 serum samples tested by ELISA with the bovine foamy virus (BFV) Gag and Bet antigens, 23 sera showed increased reactivity to at least one of them. High reactive sera represented 30% of bison samples and 7.5% of deer specimens. Eleven of the ELISA-positives were also strongly positive in immunoblot analyses. The peripheral blood DNA of seroreactive animals was tested by semi-nested PCR. The specific 275 bp fragment of the pol gene was amplified only in one sample collected from a red deer and the analysis of its sequence showed the highest homology for European BFV isolates. Such results may suggest the existence of a new FV reservoir in bison as well as in deer populations. Whether the origin of such infections stems from a new FV or is the result of BFV inter-species transmission remains to be clarified.

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“…The DSp for the IDEXX ELISA kit was 93.48%, while the DSp was 82.31%, with 95 samples (17.69%) tested to be false positive in the IDVET ELISA kit compared with the relative gold standard. A possible reason was that the use of a purified recombinant antigen significantly decreased false-positive reactions to other proteins and increased the specificity of the assay [32–34]. Methodologically, the IDEXX ELISA kit was an indirect ELISA, while the IDVET ELISA kit was a competition ELISA.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum

Jing

Liu

et al. 2019

BMC Vet Res

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BackgroundAkabane disease (AD), a barrier to international trade for endemic areas with far economic impact on the countries, is caused by Akabane virus (AKAV). Commercial enzyme-linked immunosorbent assay (ELISA) is a commonly used diagnostic technique for AKAV infection, including the IDEXX and IDVET ELISA kits. However, the comparative evaluation of the IDEXX and IDVET ELISA kits has not been published. The object of this study was to evaluate the test performance of the two commercial ELISA kits in detecting serum anti-AKAV antibodies in cattle.ResultsWith virus neutralization test (VNT) as the “relative gold standard”, the diagnostic sensitivity (DSe) was 80.39% (123/153) and 93.46% (143/153) for the IDEXX and IDVET ELISA kit, when suspect samples were included. The diagnostic specificity (DSp) for the IDEXX and IDVET ELISA kit was 93.48% (502/537) and 82.31% (442/537), respectively.ConclusionBoth of the tested ELISA kits could be applied to detect antibodies against AKAV in cattle serum. The IDVET ELISA kit had a higher DSe. The IDEXX ELISA kit possessed the higher DSp. These results have important implications if the kits are used to screen herds or individual cattle in surveillance programs, or at border crossings for import-export inspection and quarantine.

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Development of a recombinant protein-based ELISA for detection of antibodies against bovine foamy virus

Cited by 13 publications

References 20 publications

Bovine Foamy Virus: Shared and Unique Molecular Features In Vitro and In Vivo

Bovine Foamy Virus: Shared and Unique Molecular Features In Vitro and In Vivo

Infection with Foamy Virus in Wild Ruminants—Evidence for a New Virus Reservoir?

Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum

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