Development of a Recombinant Epsilon Toxoid Vaccine against Enterotoxemia and Its Use as a Combination Vaccine with Live Attenuated Sheep Pox Virus against Enterotoxemia and Sheep Pox

Chandran, Dev; Naidu, Sureddi Satyam; Parthasarathy, S.; Rani, Gudavalli Sudha; Vijayan, Shahana Pallichera; Mathur, Deepika; Garg, Lalit C.; Srinivasan, V.A.

doi:10.1128/cvi.00013-10

Cited by 32 publications

(30 citation statements)

References 22 publications

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“…A vast number of studies have focused on ETX due to the fact it is the third most potent toxin know and, therefore, is considered a potential biowarfare threat according to the Centers for Disease Control and Prevention (CDC), USA [94]. Even though it is highly lethal to humans, ETX is more prevalent in cases of enterotoxemia in ovine, caprine, and bovine.…”

Section: Epsilon Toxin (Etx)mentioning

confidence: 99%

“…This strategy also proved to be effective in immune prophylaxis against tetanus [101]. Chandran et al [94] used different amounts of formaldehyde-inactivated rETX per dose (50, 100, 200, 300, and 500 µg), as well as 200 µg of rETX co-administered with attenuated Sheep Pox Virus (SPV). The use of 50 µg and 100 µg per dose, induced antitoxin ETX titers of 3 and 5 IU/mL, respectively.…”

Section: Epsilon Toxin (Etx)mentioning

confidence: 99%

“…The strains of E. coli BL21 (DE3) pLysS™, Nova Blue (DE3) pLysS™, and Rosetta™ are the most commonly used, employing the pET22b and pET11a vectors. Chandran et al [94] and Goswami et al [102] used E. coli M15 and the pQE32 vector. This vector has an ampicillin resistance gene, and the expression of the target gene was under the control of the T5 promoter.…”

Section: Epsilon Toxin (Etx)mentioning

confidence: 99%

“…Numerous studies have described the use of recombinant toxins in illness immunoprophylaxis caused by C. perfringens in food animals (Table 5) [22,26,68,69,94,104]. Jiang et al [105] evaluated the treatment of calves using a trivalent vaccine that contained 300 µg of each antigen (rCPA (247–370) , rCPB and rETX) emulsified in oil adjuvant ISA 15A VG.…”

Section: Immunogenicity Rcpa Rcpb and Retx In Farm Animalsmentioning

confidence: 99%

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Recombinant Alpha, Beta, and Epsilon Toxins of Clostridium perfringens: Production Strategies and Applications as Veterinary Vaccines

Ferreira

Moreira

Cunha

et al. 2016

Toxins

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Clostridium perfringens is a spore-forming, commensal, ubiquitous bacterium that is present in the gastrointestinal tract of healthy humans and animals. This bacterium produces up to 18 toxins. The species is classified into five toxinotypes (A–E) according to the toxins that the bacterium produces: alpha, beta, epsilon, or iota. Each of these toxinotypes is associated with myriad different, frequently fatal, illnesses that affect a range of farm animals and humans. Alpha, beta, and epsilon toxins are the main causes of disease. Vaccinations that generate neutralizing antibodies are the most common prophylactic measures that are currently in use. These vaccines consist of toxoids that are obtained from C. perfringens cultures. Recombinant vaccines offer several advantages over conventional toxoids, especially in terms of the production process. As such, they are steadily gaining ground as a promising vaccination solution. This review discusses the main strategies that are currently used to produce recombinant vaccines containing alpha, beta, and epsilon toxins of C. perfringens, as well as the potential application of these molecules as vaccines for mammalian livestock animals.

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Section: Epsilon Toxin (Etx)mentioning

confidence: 99%

Section: Epsilon Toxin (Etx)mentioning

confidence: 99%

Section: Epsilon Toxin (Etx)mentioning

confidence: 99%

Section: Immunogenicity Rcpa Rcpb and Retx In Farm Animalsmentioning

confidence: 99%

See 2 more Smart Citations

Recombinant Alpha, Beta, and Epsilon Toxins of Clostridium perfringens: Production Strategies and Applications as Veterinary Vaccines

Ferreira

Moreira

Cunha

et al. 2016

Toxins

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show abstract

“…Recently, an inactivated whole-cell vaccine was developed and commercialised for the control and eradication of ET, however major disadvantage of this vaccine is local reactions at the site of inoculation. Recently, for sheep combined aluminum hydroxide adjuvanted epsilon toxoid (recombinant) and live attenuated freeze-dried sheep pox vaccine is developed (Chandran et al, 2010).…”

Section: Enterotoxemia (Et)mentioning

confidence: 99%

Prevalence, diagnosis, management and control of important diseases of ruminants with special reference to indian scenario

Saminathan¹,

Rana²,

Ramakrishnan³

et al. 2016

JEBAS

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India possess huge livestock population, which is endangered by different endemic infectious diseases (bacterial, viral, protozoan and parasitic), which collectively causes significant economic losses to the landless poor farming community. Infectious diseases impose economic losses by causing morbidity, mortality, decreased production (milk, meat, wool etc.), decreased feed conversion ratio which results in reduced weight gain, decreased draught power and fertility. Furthermore, economic burden is also due to the cost of treatment, abortion, consequences on internal livestock movement, germplasm and international trade. In addition, some of the diseases are zoonotic and inflicts considerable impact on public health. Uncertain agrarian climate, unpredictable weather, drought, floods, migration of livestock, scarcity of fodders, and unhygienic zoo-sanitary and healthcare practices together resulted in endemicity of diseases ultimately leads to more incidence and prevalence of livestock and poultry diseases throughout the year. Synchronized monitoring and surveillance of disease throughout the country is a fundamental requirement for sustainable livestock production. With fairly developed telecommunication in India, following technologies like interactive voice response system, SMS through mobile/cell phones and toll-free landline phones (voice mail) are required for enhancing the effectiveness and efficiency of

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Immunogenic and neutralization efficacy of recombinant perfringolysin O of Clostridium perfringens and its C-terminal receptor-binding domain in a murine model

et al. 2022

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Clostridium perfringens is a Gram-positive anaerobe ubiquitously present in different environments, including the gut of humans and animals. C. perfringens have been classified in the seven toxinotypes based on the secreted toxins that cause different diseases in humans and animals. Perfringolysin O (PFO), a cholesterol-dependent pore-forming cytolysin, is one of the potent toxins secreted by almost all C. perfringens isolates . The PFO acts in synergy with α-toxin in the progression of gas gangrene in humans and necrohemorrhagic enteritis in the calves. C. perfringens infections spread very fast, and the animals die within a few hours of the onset of infection. This necessitates the use of vaccines to control clostridial infections. Though the vaccine potential of other toxins has been reported, PFO has remained unexplored. The present study describes the immunogenic and protective potential of native recombinant PFO (WTrPFO). Since the PFO is toxic to the host cells, the non-toxic C-terminal domain of PFO (rPFOC-ter) was also assessed for its immunogenicity and protective efficacy. Immunization of mice with the purified soluble recombinant histidine-tagged WTrPFO and rPFOC-ter, expressed in E. coli , generated robust mixed immune response and T cell memory. Pre-incubation of the WTrPFO with anti-WTrPFO and rPFOC-ter antisera negated its hemolytic activity in mice RBCs, as well as its cytotoxic effect in mice peritoneal macrophages in vitro . Thus, immunization with the WTrPFO and its non-toxic C-terminal domain generated neutralizing antibodies, suggesting their vaccine potential against the PFO. Thus, the non-toxic C-terminal domain of PFO could serve as an alternative to PFO as a vaccine candidate. Supplementary Information The online version contains supplementary material available at 10.1007/s12026-021-09254-9.

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Development of a Recombinant Epsilon Toxoid Vaccine against Enterotoxemia and Its Use as a Combination Vaccine with Live Attenuated Sheep Pox Virus against Enterotoxemia and Sheep Pox

Cited by 32 publications

References 22 publications

Recombinant Alpha, Beta, and Epsilon Toxins of Clostridium perfringens: Production Strategies and Applications as Veterinary Vaccines

Recombinant Alpha, Beta, and Epsilon Toxins of Clostridium perfringens: Production Strategies and Applications as Veterinary Vaccines

Prevalence, diagnosis, management and control of important diseases of ruminants with special reference to indian scenario

Immunogenic and neutralization efficacy of recombinant perfringolysin O of Clostridium perfringens and its C-terminal receptor-binding domain in a murine model

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