Development of a Real-Time Reverse-Transcription PCR for Detection of Newcastle Disease Virus RNA in Clinical Samples

Wise, Mark G.; Suarez, David L.; Seal, Bruce S.; Pedersen, Janice C.; Senne, Dennis A.; King, Daniel J.; Kapczynski, Darrell R.; Spackman, Erica

doi:10.1128/jcm.42.1.329-338.2004

Cited by 416 publications

(356 citation statements)

References 30 publications

Supporting

Mentioning

335

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, isolates of other avian paramyxoviruses were tested in order to check assay specificity (Table 2). Reduced test sensitivity, compared to virus isolation (probably due to the presence of PCR inhibitors in feces), has been reported (Wise et al, 2004;OIE, 2004). In order to estimate the relative sensitivity of the assay, a serial dilution of a sham-inoculated fecal sample was comparatively tested with the standard virus isolation method (OIE, 2004); the RT-PCR detected 1 median egg infective dose (EID 50 ), indicating that the two methods have comparable sensitivities.…”

mentioning

confidence: 99%

“…Specific primerprobe sets for NDV M (matrix) gene (for detection of all NDV strains) and NDV F (fusion) gene (for detection of mesogenic and velogenic NDV strains) sequences were used as previously described, and validated (Wise et al, 2004). The TaqMan probes for the matrix and fusion genes were labeled 59 Yakima-Yellow/39 BHQ-1 and 59 FAM/39 TAMRA, respectively.…”

mentioning

confidence: 99%

“…The M gene primer-probe set was designed to detect a broad spectrum of NDV genotypes and was tested with wild bird samples (Wise et al, 2004); nonetheless, it was originally developed for virus detection in chickens, and therefore might not detect every genotype present in wildlife, or it might show varying sensitivity between bird species. This factor, in concomitance with an expected low overall prevalence of the virus, has to be carefully evaluated in interpreting the negative results.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Monitoring of Wild birds for Newcastle Disease Virus in Switzerland Using Real Time RT-PCR

Camenisch

Bandli

Hoop

2008

Journal of Wildlife Diseases

View full text Add to dashboard Cite

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Monitoring of Wild birds for Newcastle Disease Virus in Switzerland Using Real Time RT-PCR

Camenisch

Bandli

Hoop

2008

Journal of Wildlife Diseases

View full text Add to dashboard Cite

“…4). The lack of NDV positives in the Zimbabwe 2007/2008 samples may be attributed to the inability of the Wise et al assay [32], targetingthe matrix (M) gene to detect class I NDVs. By contrast, the Fuller et al assay [33] (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…were tested by the rRT-PCR method described by Wise et al [32] whereas samples collected between November 2009 and May 2010 were tested using the method described by Fuller et al [33]. All rRT-PCRs were run on an Applied Biosystem's StepOnePlus platform (Life Technologies, USA).…”

Section: Samples Collected Between May 2007 and March 2009mentioning

confidence: 99%

Empirical analysis suggests continuous and homogeneous circulation of Newcastle disease virus in a wide range of wild bird species in Africa

et al. 2014

View full text Add to dashboard Cite

SUMMARYNewcastle disease (ND) is one of the most important poultry diseases worldwide and can lead to annual losses of up to 80% of backyard chickens in Africa. All bird species are considered susceptible to ND virus (NDV) infection but little is known about the role that wild birds play in the epidemiology of the virus. We present a long-term monitoring of 9000 wild birds in four African countries. Overall, 3·06% of the birds were PCR-positive for NDV infection, with prevalence ranging from 0% to 10% depending on the season, the site and the species considered. Our study shows that ND is circulating continuously and homogeneously in a large range of wild bird species. Several genotypes of NDV circulate concurrently in different species and are phylogenetically closely related to strains circulating in local domestic poultry, suggesting that wild birds may play several roles in the epidemiology of different NDV strains in Africa. We recommend that any strategic plan aiming at controlling ND in Africa should take into account the potential role of the local wild bird community in the transmission of the disease.

show abstract

Accurate detection of Newcastle disease virus using proximity‐dependent DNA aptamer ligation assays

et al. 2021

View full text Add to dashboard Cite

Detecting viral antigens at low concentrations in field samples can be crucial for early veterinary diagnostics. Proximity ligation assays (PLAs) in both solution and solid-phase formats are widely used for high-performance protein detection in medical research. However, the affinity reagents used, which are mainly poly-and monoclonal antibodies, play an important role in the performance of PLAs. Here, we have established the first homogeneous and solid-phase proximity-dependent DNA aptamer ligation assays for rapid and accurate detection of Newcastle disease virus (NDV). NDV is detected by a pair of extended DNA aptamers that, upon binding in proximity to proteins on the envelope of the virus, are joined by enzymatic ligation to form a unique amplicon that can be sensitively detected using real-time PCR. The sensitivity, specificity, and reproducibility of the assays were validated using 40 farm samples. The results demonstrated that the developed homogeneous and solid-phase PLAs, which use NDV-selective DNA aptamers, are more sensitive than the sandwich enzymatic-linked aptamer assay (ELAA), and have a comparable sensitivity to real-time reverse transcription PCR (rRT-PCR) as the gold standard detection method. In addition, the solid-phase PLA was shown to have a greater dynamic range with improved lower limit of detection, upper-and lower limit of quantification, and minimal detectable dose as compared with those of ELAA and rRT-PCR. The specificity of PLA is shown to be concordant with rRT-PCR.

show abstract

Development of a Real-Time Reverse-Transcription PCR for Detection of Newcastle Disease Virus RNA in Clinical Samples

Cited by 416 publications

References 30 publications

Monitoring of Wild birds for Newcastle Disease Virus in Switzerland Using Real Time RT-PCR

Monitoring of Wild birds for Newcastle Disease Virus in Switzerland Using Real Time RT-PCR

Empirical analysis suggests continuous and homogeneous circulation of Newcastle disease virus in a wide range of wild bird species in Africa

Accurate detection of Newcastle disease virus using proximity‐dependent DNA aptamer ligation assays

Contact Info

Product

Resources

About