Abstract:This study aimed to identify important mycotoxigenic fungi and accurate detection of mycotoxin in stored maize grains using molecular methods. The current study also optimised the real-time PCR (RT-PCR) assay. The melting curve was established to identify isolated fungal species of
Aspergillus
(4),
Fusarium
(3),
Penicillium
(3), and
Alternaria
(one). A multiplex polymerase chain reaction (mPCR) technique was developed f… Show more
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