Development of a real-time NASBA assay for the detection of Campylobacter jejuni cells

Cools, Ils; Uyttendaele, Mieke; D’Haese, Eva; Nelis, Hans; Debevere, Johan

doi:10.1016/j.mimet.2005.12.004

Cited by 16 publications

(6 citation statements)

References 24 publications

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“…Alternatively, Nucleic Acid Sequence Based Amplification (NASBA) technology can be applied, which has as advantage above real-time PCR assays that it is fast to perform; i.e. 60 minutes for NASBA compared to up to four hours for real-time PCR [ 21 , 22 ]. NASBA assays, using molecular beacons as detection probes, has been developed for different Plasmodium species and has shown to be very sensitive with a detection limit of 20 parasites/ml blood [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Plasmodium spp. in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification

Marangi

Tullio

Mens

et al. 2009

Malar J

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Section: Introductionmentioning

confidence: 99%

Prevalence of Plasmodium spp. in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification

Marangi

Tullio

Mens

et al. 2009

Malar J

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“…Both the NASBA and the reverse transcription conventional and real time PCR techniques have been used for developing diagnostic tests to detect viable pathogenic microorganisms [157][158][159]. Since NASBA is performed in isothermal conditions, it does not require the use of a thermocycler.…”

Section: Detecting Stressed or Injured Pathogens: Ema And Pma Pcr/reamentioning

confidence: 99%

Nucleic Acid-Based Methods to Identify, Detect and Type Pathogenic Bacteria Occurring in Milk and Dairy Products

Fusco¹,

Quero²

2012

Structure and Function of Food Engineering

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“…This method particularly suited to the detection of RNA viruses because an RNA polymerase is used to amplify RNA without conversion to cDNA. This assay has been evaluated with several foodborne pathogens [66][67][68], and it was indicated that NASBA was a sensitive, specific, and rapid analysis method.…”

Section: Nucleic Acid Sequence-based Amplification (Nasba)mentioning

confidence: 99%

Molecular methods for the detection and characterization of foodborne pathogens

Shi

Long

Suo

2010

Pure and Applied Chemistry

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The surveillance of foodborne pathogens in food industries has shown the urgent need for rapid and dependable methods to detect and characterize the organisms in food and environments of clinical and epidemiologic importance. Recent studies on rapid methods in microbiology have been focused on biochemical characterization, immunoassays, and molecular methods. Many molecular methods have been developed and applied to the detection and characterization of foodborne pathogens in laboratories and food industries. They can be mainly divided into DNA banding pattern-based tests and DNA sequence-based tests. The former includes nucleic acid hybridization, polymerase chain reaction (PCR), amplified restriction length polymorphism, and randomly amplified polymorphic DNA, etc. Most of these methods in commercial applications are based on PCR or hybridization techniques. The principle, characteristics, and application of molecular methods for the detection and characterization of foodborne pathogens were reviewed in this article.

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Development of a real-time NASBA assay for the detection of Campylobacter jejuni cells

Cited by 16 publications

References 24 publications

Prevalence of Plasmodium spp. in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification

Prevalence of Plasmodium spp. in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification

Nucleic Acid-Based Methods to Identify, Detect and Type Pathogenic Bacteria Occurring in Milk and Dairy Products

Molecular methods for the detection and characterization of foodborne pathogens

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