Development of a Real Time PCR system for detection of ochratoxin A-producing strains of the Aspergillus niger aggregate

Presence of ochratoxin A on the surface of dry-cured Iberian ham after initial fungal growth in the drying stage

“…For this, specific primers based on the ochratoxin A polyketide synthase gene (otapksPN) previously described, were used to detect three different OTA-producing species (Table 1): P. nordicum (Geisen, Mayer, Karolewiez, & Färber, 2004), P. verrucosum (Schmidt-Heydt, Richter, Michulec, Buttinger, & Geisen, 2008) and A. niger aggregate (also A. ochraceus) (Castellá & Cabañes, 2011). The The amplification program used was: 1 cycle of 10 min at 95°C and 40 cycles of 95°C for 15 s and 60°C for 1 min.…”

Identification of ochratoxin A producing Aspergillus carbonarius and A. niger clade isolated from grapes using the loop-mediated isothermal amplification (LAMP) reaction

“…; Storari et al . ; Castellá and Cabañes ). These two PKS genes could be targeted to specifically detect ochratoxigenic strains of A. carbonarius and of the A. niger clade in the field and in foodstuff.…”

“…These two PKS genes could be targeted to specifically detect ochratoxigenic strains of A. carbonarius and of the A. niger clade in the field and in foodstuff. In fact, Castellá and Cabañes () used an15g07920 as molecular target in a real‐time PCR system to detect ochratoxigenic A. niger strains on maize. Recently, Yamada et al .…”

Recent advances in ochratoxin A-producing fungi detection based on PCR methods and ochratoxin A analysis in food matrices

“…Both procedures were successfully used to monitor, in a single reaction, the presence of both OTA-producing species in grapes. Recently, a new qPCR procedure has been developed for the rapid and specific detection and quantification of A. niger aggregate using as a target the pks gene (Castellá & Cabañes, 2011). Among a total of 91 strains assayed in this study, 40 were isolated from foods samples such as wine grape, corn, soya and feedstuffs.…”