Development of a real time RT-PCR to detect and type ovine pestiviruses

Willoughby, Kim; Valdazo-González, Begoña; Maley, Madeleine; Gilray, Janice; Nettleton, P. F.

doi:10.1016/j.jviromet.2005.10.007

Cited by 53 publications

(36 citation statements)

References 21 publications

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“…BD is caused by only BvDv-1 in norway (Sandvik et al, 2002) and Sweden (vilcek et al, 1997), only BDv in Australia, new Zealand and Spain (vilcek et al, 1998;valdazo-Gonzalez et al 2006), BDv and BvDv-1 in u.k. (Willoughby et al, 2006) and Austria (krametter-Froetscher et al, 2010), only BvDv-2 in korea (kim et al, 2006), BDv, BvDv-1, and BvDv-2 in uSA (Sullivan et al, 1997) and Italy (Pratelli et al, 2001;Giammarioli et al, 2011). The results provided in this study and previous reports suggest that BD is caused by both, BvDv-1 and BvDv-2 in India.…”

Section: Discussionsupporting

confidence: 70%

“…All the seven sheep and five goat pestiviruses studied here were identified as BvDv-1 on the basis of both 5ʹ-utr and n pro sequence analysis, which demonstrates that BvDv-1 infections are widespread in small ruminants. Prevalence of BvDv-1 in sheep and goats is, however, not uncommon, as ovine/ caprine pestiviruses have been found to be BvDv-1 in u.k. (vilcek et al, 1997;Willoughby et al, 2006), uSA (Sullivan et al, 1997), Italy (Pratelli et al, 2001), Sweden (vilcek et al, 1997), norway (Sandvik et al, 2002) and Austria (krametterFroetscher et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Genetic variety of bovine viral diarrhea virus 1 strains isolated from sheep and goats in India

Mishra¹,

Pitale²,

Rajukumar³

et al. 2012

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Summary. -Antigenic and genetic typing of pestiviruses isolated from Indian sheep and goats was carried out. testing of 1777 sheep and 1026 goat blood samples collected between 2004 and 2008 resulted in isolation of twelve pestiviruses, seven from sheep and five from goats. All of them were antigenically typed as bovine viral diarrhea virus 1 (BvDv-1). Both the partial 5ʹ-utr and entire non-structural autoprotease (n pro ) gene of the pestiviruses were amplified by rt-Pcr and sequenced. The phylogenetic analysis confirmed all twelve sheep and goat pestiviruses as BvDv-1 and they were further classified into two subtypes, BvDv-1b (seven) and . This is for the first time that BvDv-1c was detected in sheep and goats. however, no association between the subtype and geographic area of origin was observed. Although closely related, BvDv-1b and BvDv-1c isolates of sheep and goats were placed in a different clade than previously reported Indian BvDv-1b/ BvDv-1c isolates. This study confirmed widespread prevalence of BvDv-1 in Indian sheep and goats that has significance in the epidemiology of bovine viral diarrhea.Keywords: bovine viral diarrhea virus; BvDv-1; goat; n pro ; genetic typing; sheep; 5ʹ-utr e-mail: mishranir@rediffmail.com; phone: +91-755-2759204. Abbreviations: BD = border disease; BDv = BD virus; BvDv = bovine viral diarrhea viruses; BvDv-1,2 = bovine viral diarrhea virus 1 and 2; IPMA = indirect immunoperoxidase monolayer assay; MAb(s) = monoclonal antibody(ies); ncp = non-cytopathic; n pro = non-structural autoprotease; SFtr = sheep fetal thymus cells

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Genetic variety of bovine viral diarrhea virus 1 strains isolated from sheep and goats in India

Mishra¹,

Pitale²,

Rajukumar³

et al. 2012

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“…The studies of Willoughby et al [32] indicate that colostral antibodies can mask the presence of BDV in pestivirus infected animals and misrepresent the presence of BDV infected lambs. This observation might be explained by the presence of colostral antibodies in the dams.…”

Section: Discussionmentioning

confidence: 99%

Comparative Investigation of Border Disease Virus Infection in Sheep Flocks with Abortion Problems in Konya Province

Avcı

Yavru

2014

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Abstract:The aim of the present study was to determine the serological and virological status of animals persistently infected (PI) with border disease virus (BDV), with focus on the prevalence of pestiviruses in sheep and lambs. This study is the first to our knowledge, to investigate the role of viral infections in abortions in the sheep population of Konya province. The prevalence of antibodies against BDV was as follows: sheep, 79%; lambs, 43.4%; and rams, 6%. Of 1000 leukocyte (sheep) samples examined with a direct ELISA, 11 (1.1%) (first sampling) were antibody-positive. Three sheep were determined to be PI and were sacrificed. Of 1011 sheep leukocyte samples, 14 were examined using a direct immunoperoxidase (IP) assay for BDV antigen, and 11 of 327 (3.36%) tissue samples were positive. The BDV genome was detected in 14 of 63 (22.2%) sheep leukocyte samples and 11 of 327 (3.36%) tissue samples by one-step RT-PCR. The BDV genome could not be detected in any vaginal swab samples. The sensitivity and specificity rates between direct ELISA and direct IP were respectively 100% and 98.8% and the values were, respectively, 78.57% and 98.6% between direct ELISA and one-step RT-PCR and 100% and 97.5% between one-step RT-PCR and direct IP. In conclusion it was determinate that BDV has an important role as an etiologic agent in abortion causes in sheep population in Konya.

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“…Persiste enfekte koyunların tespitinde nested ve real time RT-PCR tekniklerinin kullanımı hızlı genotiplendirme ve ayırıcı teşhis için avantaj sağlamaktadır (43).…”

Section: Tanıunclassified

Sınır Hastalığı (Border Disease)

Oğuzoğlu¹

2008

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu makalede, küçükbaş hayvan sürülerinde büyük ekonomik kayıplara yol açan, abortlar ve kongenital anomalili yavru doğumu ile karakterize olan Sınır Hastalığı'nın (Border Disease, BD) etiyolojik özellikleri, etkenin bulaşma yolları, meydana getirdiği klinik-patolojik bulgular, patogenez mekanizması, teşhisi ve mücadele yolları hakkındaki güncel bilgiler sunulmuştur.Anahtar sözcükler: Sınır Hastalığı, keçi, koyun, Pestivirus. Border DiseaseSummary: In this article is complied the current knowledge about etiological agent, transmission ways, clinical and pathological findings, pathogenesis, diagnosis and control of Border Disease (BD) infection, characterised by abortus and birth with congenital abnormality, which caused in small ruminant flocks great economicaly losses.

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Development of a real time RT-PCR to detect and type ovine pestiviruses

Cited by 53 publications

References 21 publications

Genetic variety of bovine viral diarrhea virus 1 strains isolated from sheep and goats in India

Genetic variety of bovine viral diarrhea virus 1 strains isolated from sheep and goats in India

Comparative Investigation of Border Disease Virus Infection in Sheep Flocks with Abortion Problems in Konya Province

Sınır Hastalığı (Border Disease)

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