Development of a rapid, high-throughput method for quantification of zeaxanthin in Chinese wolfberry using HPLC–DAD

Zhao, Liuqing; Qiu, Zhiqiang; Narasimhamoorthy, B.; Greaves, John

doi:10.1016/j.indcrop.2013.02.008

Cited by 23 publications

(11 citation statements)

References 19 publications

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“…However, with the frequent mixing of fruits from different origins in the market in recent years, the quality of Ningxia wolfberries is difficult to guarantee. According to most researches, the geographical origins of Chinese wolfberries can be identified based on observing the shape of the wolfberries and using chemical methods to detect internal quality, however, these methods are time-consuming, destructive to the samples and with low detection accuracy [ 4 – 5 ]. Therefore, establishing rapid, nondestructive and high-accurate methods to trace the origin of Chinese wolfberries is urgent.…”

Section: Introductionmentioning

confidence: 99%

Application of near-infrared hyperspectral imaging to discriminate different geographical origins of Chinese wolfberries

et al. 2017

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Near-infrared (874–1734 nm) hyperspectral imaging (NIR-HSI) technique combined with chemometric methods was used to trace origins of 1200 Chinese wolfberry samples, which from Ningxia, Inner Mongolia, Sinkiang and Qinghai in China. Two approaches, named pixel-wise and object-wise, were investigated to discriminative the origin of these Chinese wolfberries. The pixel-wise classification assigned a class to each pixel from individual Chinese wolfberries, and with this approach, the differences in the Chinese wolfberries from four origins were reflected intuitively. Object-wise classification was performed using mean spectra. The average spectral information of all pixels of each sample in the hyperspectral image was extracted as the representative spectrum of a sample, and then discriminant analysis models of the origins of Chinese wolfberries were established based on these average spectra. Specifically, the spectral curves of all samples were collected, and after removal of obvious noise, the spectra of 972–1609 nm were viewed as the spectra of wolfberry. Then, the spectral curves were pretreated with moving average smoothing (MA), and discriminant analysis models including support vector machine (SVM), neural network with radial basis function (NN-RBF) and extreme learning machine (ELM) were established based on the full-band spectra, the extracted characteristic wavelengths from loadings of principal component analysis (PCA) and 2nd derivative spectra, respectively. Among these models, the recognition accuracies of the calibration set and prediction set of the ELM model based on extracted characteristic wavelengths from loadings of PCA were higher than 90%. The model not only ensured a high recognition rate but also simplified the model and was conducive to future rapid on-line testing. The results revealed that NIR-HSI combined with PCA loadings-ELM could rapidly trace the origins of Chinese wolfberries.

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Section: Introductionmentioning

confidence: 99%

Application of near-infrared hyperspectral imaging to discriminate different geographical origins of Chinese wolfberries

et al. 2017

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“…A daily goji berry serving provided 28.8 mg of Z, which was substantially higher than the 4 mg of Z present in the supplement. Although sufficient extraction of L from our goji berry samples could not be obtained, previous work by others estimated a L content of 0.15 mg in 28 g of goji berries from six different goji berry samples collected in the Ningxia province of China, the same region from which the goji berries used in this study were obtained [25].…”

Section: Resultsmentioning

confidence: 94%

Goji Berry Intake Increases Macular Pigment Optical Density in Healthy Adults: A Randomized Pilot Trial

Holt

Keen

et al. 2021

Nutrients

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Age-related macular degeneration (AMD) is the third leading cause of blindness worldwide. Macular pigment optical density (MPOD), a biomarker for AMD, is a non-invasive measure to assess risk. The macula xanthophyll pigments lutein (L) and zeaxanthin (Z) protect against blue light and provide oxidant defense, which can be indexed by MPOD. This study examined the effects of Z-rich goji berry intake on MPOD and skin carotenoids in healthy individuals. A randomized, unmasked, parallel-arm study was conducted with 27 participants, aged 45–65, who consumed either 28 g of goji berries or a supplement containing 6 mg L and 4 mg Z (LZ), five times weekly for 90 days. After 90 days, MPOD was significantly increased in the goji berry group at 0.25 and 1.75 retinal eccentricities (p = 0.029 and p = 0.044, respectively), while no changes were noted in the LZ group. Skin carotenoids were significantly increased in the goji berry group at day 45 (p = 0.025) and day 90 (p = 0.006), but not in the LZ group. Regular intake of goji berries in a healthy middle-aged population increases MPOD may help prevent or delay the development of AMD.

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“…According to Lu's study on 13 Goji berry samples from different regions in China, the content of zeaxanthin‐dipalmitate was more than 100–500 times higher than zeaxanthin [28]. In order to get more zeaxanthin, the saponification is often used during the extraction which could convert carotenoid esters to free carotenoids and simultaneously remove chlorophylls, lipids, and water‐soluble impurities [29]. During the saponification the carotenoid isomerization and degradation could be avoided by the addition of antioxidants, limited exposure to light and oxygen, nitrogen flushing, and rapid cooling of the mixture after saponification [26].…”

Section: Resultsmentioning

confidence: 99%

Recovery and recycling of solvent of counter‐current chromatography: The sample of isolation of zeaxanthin in the Lycium barbarum L. fruits

Duan

Quan

Huang

et al. 2021

J of Separation Science

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An efficient method of recovering and recycling solvent for counter‐current chromatography was established by which zeaxanthin was separated from Lycium barbarum L. fruits. A column with activated carbon combined with high performance counter‐current chromatography formed the recovering and recycling solvent system. Using the solvent system of n‐hexane–ethyl acetate–ethanol–water (8:2:7:3, v/v) from the references, five injections were performed with an almost unchanged purity of zeaxanthin (80.9, 81.2, 81.5, 81.3, and 80.2% respectively) in counter‐current chromatography separation. Meanwhile, the mobile phase reduced by half than conventional counter‐current chromatography. By this present method, an effective improvement of counter‐current chromatography solvent utilization was achieved.

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Development of a rapid, high-throughput method for quantification of zeaxanthin in Chinese wolfberry using HPLC–DAD

Cited by 23 publications

References 19 publications

Application of near-infrared hyperspectral imaging to discriminate different geographical origins of Chinese wolfberries

Application of near-infrared hyperspectral imaging to discriminate different geographical origins of Chinese wolfberries

Goji Berry Intake Increases Macular Pigment Optical Density in Healthy Adults: A Randomized Pilot Trial

Recovery and recycling of solvent of counter‐current chromatography: The sample of isolation of zeaxanthin in the Lycium barbarum L. fruits

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