Development of a rapid detection method for real-time fluorescent quantitative PCR of Salmonella spp. and Salmonella Enteritidis in ready-to-eat fruits and vegetables

Wan, Jiajia; Zheng, Le; Kong, Lingqi; Lu, Zhaoxin; Tao, Yang; Feng, Zhiyang; Lv, Fengting; Meng, Fanqiang; Bie, Xiaomei

doi:10.1016/j.lwt.2021.111837

Cited by 15 publications

(7 citation statements)

References 33 publications

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“…Clearly, it is of prime importance to identify foodborne pathogens in order to prevent them from causing significant harm to human and environmental health [42]. Culturing, biochemical analysis and immunoassay are the three most common conventional methods for determining foodborne pathogens [43,44]. However, a number of drawbacks can be found with these techniques, which include the time-consuming nature of performing these techniques, the length of the identification cycle, and the high costs and high level of operator skill required [45,46].…”

Section: Introductionmentioning

confidence: 99%

An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

et al. 2023

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Pathogens found in food are believed to be the leading cause of foodborne illnesses; and they are considered a serious problem with global ramifications. During the last few decades, a lot of attention has been paid to determining the microorganisms that cause foodborne illnesses and developing new methods to identify them. Foodborne pathogen identification technologies have evolved rapidly over the last few decades, with the newer technologies focusing on immunoassays, genome-wide approaches, biosensors, and mass spectrometry as the primary methods of identification. Bacteriophages (phages), probiotics and prebiotics were known to have the ability to combat bacterial diseases since the turn of the 20th century. A primary focus of phage use was the development of medical therapies; however, its use quickly expanded to other applications in biotechnology and industry. A similar argument can be made with regards to the food safety industry, as diseases directly endanger the health of customers. Recently, a lot of attention has been paid to bacteriophages, probiotics and prebiotics most likely due to the exhaustion of traditional antibiotics. Reviewing a variety of current quick identification techniques is the purpose of this study. Using these techniques, we are able to quickly identify foodborne pathogenic bacteria, which forms the basis for future research advances. A review of recent studies on the use of phages, probiotics and prebiotics as a means of combating significant foodborne diseases is also presented. Furthermore, we discussed the advantages of using phages as well as the challenges they face, especially given their prevalent application in food safety.

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Section: Introductionmentioning

confidence: 99%

An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

et al. 2023

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“…qPCR operates by adding fluorescent-labeled probes or fluorescent substances into the PCR system and monitors the accumulation of fluorescence signals in the whole PCR amplification process in real-time through the instrument. Finally, the method for quantitative analysis of samples with unknown concentrations was carried out through standard curves ( Fu et al, 2020 ; Baoutina and Bhat, 2021 ; Wan et al, 2021 ). Moreover, Chen et al (2017) presented a new POCT system based on magnetic nanoparticles that enable sample in-answer out (SIAO) automated real-time testing for pathogens ( Figure 3A ).…”

Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

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“…Therefore, achieving sensitive and accurate detection of SE is of high importance. Various methods including enzyme-linked immunosorbent assays [ 23 , 24 ], electrochemistry [ 25 ], fluorescence [ 26 , 27 ] and electrochemiluminescence [ 28 , 29 ] assays have been explored for detection of SE. Especially the ECL technique has been considered as a superior choice because of its remarkable sensitivity and controllability.…”

Section: Introductionmentioning

confidence: 99%

Facial Preparation of Cyclometalated Iridium (III) Nanowires as Highly Efficient Electrochemiluminescence Luminophores for Biosensing

et al. 2023

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In this study, highly efficient ECL luminophores composed of iridium complex-based nanowires (Ir–NCDs) were synthesized via covalently linking bis(2-phenylpyridine)-(4-carboxypropyl-2,2′-bipyridyl) iridium(III) hexafluorophosphate with nitrogen-doped carbon quantum dots (NCDs). The ECL intensity of the nanowires showed a five-fold increase in ECL intensity compared with the iridium complex monomer under the same experimental conditions. A label-free ECL biosensing platform based on Ir–NCDs was established for Salmonella enteritidis (SE) detection. The ECL signal was quenched linearly in the range of 102–108 CFU/mL for SE with a detection limit of 102 CFU/mL. Moreover, the relative standard deviations (RSD) of the stability within and between batches were 0.98% and 3.9%, respectively. In addition, the proposed sensor showed high sensitivity, selectivity and stability towards SE in sheep feces samples with satisfactory results. In summary, the excellent ECL efficiency of Ir–NCDs demonstrates the prospects for Ir(III) complexes in bioanalytical applications.

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Development of a rapid detection method for real-time fluorescent quantitative PCR of Salmonella spp. and Salmonella Enteritidis in ready-to-eat fruits and vegetables

Cited by 15 publications

References 33 publications

An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Facial Preparation of Cyclometalated Iridium (III) Nanowires as Highly Efficient Electrochemiluminescence Luminophores for Biosensing

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