Development of a rapid detection method for real-time fluorescent quantitative PCR of Salmonella spp. and Salmonella Enteritidis in ready-to-eat fruits and vegetables

Wan, Jiajia; Zheng, Le; Kong, Lingqi; Lu, Zhaoxin; Tao, Yang; Feng, Zhiyang; Lv, Fengting; Meng, Fanqiang; Bie, Xiaomei

doi:10.1016/j.lwt.2021.111837

Cited by 12 publications

(4 citation statements)

References 33 publications

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“…qPCR operates by adding fluorescent-labeled probes or fluorescent substances into the PCR system and monitors the accumulation of fluorescence signals in the whole PCR amplification process in real-time through the instrument. Finally, the method for quantitative analysis of samples with unknown concentrations was carried out through standard curves ( Fu et al, 2020 ; Baoutina and Bhat, 2021 ; Wan et al, 2021 ). Moreover, Chen et al (2017) presented a new POCT system based on magnetic nanoparticles that enable sample in-answer out (SIAO) automated real-time testing for pathogens ( Figure 3A ).…”

Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

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Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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“…Therefore, achieving sensitive and accurate detection of SE is of high importance. Various methods including enzyme-linked immunosorbent assays [ 23 , 24 ], electrochemistry [ 25 ], fluorescence [ 26 , 27 ] and electrochemiluminescence [ 28 , 29 ] assays have been explored for detection of SE. Especially the ECL technique has been considered as a superior choice because of its remarkable sensitivity and controllability.…”

Section: Introductionmentioning

confidence: 99%

Facial Preparation of Cyclometalated Iridium (III) Nanowires as Highly Efficient Electrochemiluminescence Luminophores for Biosensing

et al. 2023

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In this study, highly efficient ECL luminophores composed of iridium complex-based nanowires (Ir–NCDs) were synthesized via covalently linking bis(2-phenylpyridine)-(4-carboxypropyl-2,2′-bipyridyl) iridium(III) hexafluorophosphate with nitrogen-doped carbon quantum dots (NCDs). The ECL intensity of the nanowires showed a five-fold increase in ECL intensity compared with the iridium complex monomer under the same experimental conditions. A label-free ECL biosensing platform based on Ir–NCDs was established for Salmonella enteritidis (SE) detection. The ECL signal was quenched linearly in the range of 102–108 CFU/mL for SE with a detection limit of 102 CFU/mL. Moreover, the relative standard deviations (RSD) of the stability within and between batches were 0.98% and 3.9%, respectively. In addition, the proposed sensor showed high sensitivity, selectivity and stability towards SE in sheep feces samples with satisfactory results. In summary, the excellent ECL efficiency of Ir–NCDs demonstrates the prospects for Ir(III) complexes in bioanalytical applications.

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“…NTS has more than 2,610 serovars, their host specificity and the ability to cause diseases varies among serovars [26]. Symptoms of salmonellosis include diarrhea, fever, stomach ache, vomiting, nausea, muscular or articular pain and loss of appetite [32], however, infection occasionally results in life-threatening systemic involvement especially to the patients with compromised immunity.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Non-typhoidal Salmonella (NTS) Isolated from Salad Vegetables in Terengganu, Malaysia

Haslinda

Tang

2023

Mal. J. Fund. Appl. Sci.

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Salad vegetables are the important sources of vitamins and minerals for human diet. However, salad vegetables are potential vehicles for non-typhoidal Salmonella spp. (NTS) as reported in our previous study. Such contamination posed significant food poisoning risk to consumer. The aims of present study include determination of the virulence genes prevalence in NTS isolates in samples from retail markets and farms. Besides, characterization of NTS isolates was performed to determine their genetic relatedness. Multiplex PCR assay was done to detect the presence of 15 virulence genes in 58 NTS isolates. The genetic relatedness of 33 NTS belonging to S. Weltevreden, S. Albany, S. Hvittingfoss, S. Aberdeen, S. Poona and S. Corvallis were characterized using ERIC-PCR and BOX-PCR typing methods. Results showed the presence of 13 virulence genes (spiA, pagC, msgA, invA, sipB, prgH, spaN, orgA, tolC, sitC, lpfC, sifA and sopB) among all the isolates with 53 (91.38%) isolates carried more than 11 virulence genes, while other five isolates carried the genes in the range of six to nine types. None of the isolates possessed spvB and pefA genes. Major clusters were observed at a genetic distance percentage of 90% for both ERIC- and BOX-PCR with discriminatory index of 0.917 and 0.873, respectively. In terms of source and type of sample, all NTS isolates were found diverse without clear association, thus no specific cluster was found. In summary, this study demonstrated the potential risks of NTS from salad vegetables to cause foodborne diseases and they were commonly found to be related with regard to their specific serovars.

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Development of a rapid detection method for real-time fluorescent quantitative PCR of Salmonella spp. and Salmonella Enteritidis in ready-to-eat fruits and vegetables

Cited by 12 publications

References 33 publications

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Facial Preparation of Cyclometalated Iridium (III) Nanowires as Highly Efficient Electrochemiluminescence Luminophores for Biosensing

Molecular Characterization of Non-typhoidal Salmonella (NTS) Isolated from Salad Vegetables in Terengganu, Malaysia

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