Development of a rapid and simultaneous immunochromatographic assay for the determination of zearalenone and fumonisin B1 in corn, wheat and feedstuff samples

Wang, Yuan-Kai; Shi, Yong; Zou, Qi; Sun, Jianhe; Chen, Zhi-Fei; Wang, Heng-an; Li, Shuqing; Yan, Yaxian

doi:10.1016/j.foodcont.2012.09.048

Cited by 62 publications

(43 citation statements)

References 28 publications

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“…Anti-ZEN mAb-gold conjugate and ICA test strips were prepared as per the method previously described Wang et al 2013). The sensitivity of immunoassay was prepared by spraying ZEN-OVA and goat anti-mouse mAb (1.0 mg/mL) to the nitrocellulose membrane (NC) using BioJet (Biodot, California, USA) as the test line and control line respectively.…”

Section: Ica For Measuring Anti-zen Mab Activitymentioning

confidence: 99%

Evaluation and Optimization of Three Different Immunoassays for Rapid Detection Zearalenone in Fodders

Jiang

Li²,

Yang³

et al. 2016

Food Anal. Methods

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Zearalenone (ZEN) is one of the most common contaminants in fodder with obvious reproduction toxicity and potential carcinogenicity to animals and humans. Thus, simple and sensitive methods are required for the detection of ZEN. In this work, the anti-ZEN monoclonal antibody (mAb) was prepared by hybridoma technique. Then, the mAb-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA), fluorescence polarization immunoassay (FPIA), and immunochromatographic assay (ICA) were evaluated and optimized for ZEN detection in spiked fodder samples. The ic-ELISA and FPIA showed recovery ranges from 80 to 100 %. The limit of detection (LOD) in ic-ELISA, FPIA and ICA were 0.06, 0.54, and 10 ng/mL, respectively. The detection ranges of IC 20~I C 80 were 2.89 to 115.36 ng/mL in ic-ELISA and 3.0 to 1052 ng/mL in FPIA. Amongst three immunoassays, the ic-ELISA was the most sensitive and stable, nevertheless, most time-consuming with narrower detection range. The FPIA showed good sensitivity, precision, and wide detection range, but it required specific tracer. ICA was a time-saving handy method but exhibited relatively lower precision and quantification compared to other two methods.

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Section: Ica For Measuring Anti-zen Mab Activitymentioning

confidence: 99%

Evaluation and Optimization of Three Different Immunoassays for Rapid Detection Zearalenone in Fodders

Jiang

Li²,

Yang³

et al. 2016

Food Anal. Methods

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“…This method has been used to identify the presence of zearalenone in corn, wheat and grain (Kolosova et al, 2007;Liu et al, 2012;Wang et al, 2013;Shim et al, 2009). between fluorescently labeled antigens and specific antibodies, and it enables rapid analyte determination.…”

Section: Immunochromatography (Ic) (Or Lateral Flow Immunoassay (Lfiamentioning

confidence: 99%

Analytical Procedure for the Determination of Zearalenone in Environmental and Biological Samples

Kwaśniewska

Gadzała‐Kopciuch

Cendrowski

2015

Critical Reviews in Analytical Chemistry

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The metabolism of zearalenone (ZEA) and analytical methods for determining the presence of ZEA and its metabolites are discussed in this study. Similar to phytoestrogens, solid metaloestrogens, pharmaceuticals, and selected pesticides, ZEA is a substance that displays endocrine activity. ZEA is accumulated in living organisms, and it is capable of contaminating all trophic levels of the food chain, from grain, maize, and other crop plants to human consumers. Zearalenone has a structure similar to that of estrogen (the presence of a macrocyclic lactone ring), it has an affinity for estrogen receptors, and it competes with 17β-estradiol for binding the estrogen receptor in natural pathways. As endocrine disruptors, zearalenone and its metabolites can also contribute to carcinogenic mutations associated with female secondary sex characteristics. The determination of zearalenone and its metabolites in various matrices, first of all biological and environmental samples, poses significant problems. A variety ways of extracting and purifying zearalenone, including liquid-liquid extraction and solid-phase extraction, are described. Furthermore, it describes the possibility of applying a plurality of sensitive and specific instrumental methods, chromatographic techniques (TLC, HPLC, GC) as well as other methods (immunoaffinity chromatography).

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“…On the other hand, lateral flow immunoassays (LFIAs) are cost-effective, easy to use and suitable for on-site analysis. Several LFIAs have been developed for quick and simple screening of fumonisins in various types of sample (Anfossi et al, 2010;Venkataramana et al, 2014;Wang et al, 2013;Yu et al, 2015). Nonetheless, most available LFIAs today are based on monoclonal or polyclonal IgG from mammals, which increases the cost of production and involves ethical issues of animal welfare Polyclonal IgY antibodies from egg yolk of laying hens represent an attractive alternative to monoclonal and rodent polyclonal antibodies.…”

Section: Introductionmentioning

confidence: 99%

Development of an IgY-based lateral flow immunoassay for detection of fumonisin B in maize

et al. 2019

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Fumonisins are among the most prevalent mycotoxins in maize, causing substantial economic losses and potential health risks in humans and animals. In the present study, in-house polyclonal IgY antibody against fumonisin B1 (FB ) and B2 (FB ) was applied for the development of a competitive lateral flow immunoassay detecting these mycotoxins in maize grains with the limit of detection of 4000 µg/kg, which corresponds to the maximum residue limit adopted by the European Commission. To this end, factors affecting the test performance including nitrocellulose membrane type, dilution factor of maize homogenates in running buffer, amount of detection conjugate, and incubation time between detection conjugate and samples were optimized. Under the optimal condition (UniSart CN140 nitrocellulose membrane, FB -BSA immobilized at 1 µg/cm, 1:10 dilution factor, 436 ng of gold nanoparticle conjugate, 30 minutes of incubation), the developed test could detect both FB and FB in maize with limit of detection of 4000 µg/kg, and showed no cross-reactivity to deoxynivalenol, ochratoxin A, aflatoxin B1 and zearalenone. When applied to detect FB and FB in naturally contaminated maize samples, results obtained from the developed assay were in good agreement with those from the high-performance liquid chromatography method. This lateral flow immunoassay is particularly suitable for screening of fumonisins in maize because of its simplicity and cost-effectiveness.

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Development of a rapid and simultaneous immunochromatographic assay for the determination of zearalenone and fumonisin B1 in corn, wheat and feedstuff samples

Cited by 62 publications

References 28 publications

Evaluation and Optimization of Three Different Immunoassays for Rapid Detection Zearalenone in Fodders

Evaluation and Optimization of Three Different Immunoassays for Rapid Detection Zearalenone in Fodders

Analytical Procedure for the Determination of Zearalenone in Environmental and Biological Samples

Development of an IgY-based lateral flow immunoassay for detection of fumonisin B in maize

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