Development of a rapid and sensitive immunosensor for the detection of bacteria

Verdoodt, Niels; Basso, Caroline R.; Rossi, Bruna Fernanda; Pedrosa, Valber A.

doi:10.1016/j.foodchem.2016.10.102

Cited by 57 publications

(38 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The proposed sensor analysis time (20 minutes) is comparable to the time reported in the other rapid methods . Although some of the nanotechnology‐based methods reported in Table for comparison can detect the presence of the bacteria in 1 to 5 minutes, the major advantages of the biosensor we proposed over the other rapid techniques are the simplicity of the design and the low cost of fabrication in addition to the highly electivity of bacteriophage while maintaining a rapid response (20 minutes).…”

Section: Resultsmentioning

confidence: 68%

“…To increase sensitivity and shorten testing time for food samples, several types of biosensors have been proposed using nanotechnology, the best of which are optical‐based sensors. Several optical sensors were proposed based on colorimetric, fluorescence, chemiluminescence, and plasmonic signal detection . These systems can provide reliable results in few minutes, but many of these systems are expensive, toxic (needs special handling), and/or complex in design, fabrication, and testing.…”

Section: Introductionmentioning

confidence: 99%

“…Several optical sensors were proposed based on colorimetric, fluorescence, chemiluminescence, and plasmonic signal detection. 11,[20][21][22][23] These systems can provide reliable results in few minutes, but many of these systems are expensive, toxic (needs special handling), and/or complex in design, fabrication, and testing.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Using millimeter‐waves for rapid detection of pathogenic bacteria in food based on bacteriophage

Sultan

Ali

Fahmy

et al. 2019

Engineering Reports

View full text Add to dashboard Cite

The accessibility of a rapid method for detection and identification of food‐borne pathogens is crucial for food industry worldwide. Antibiotic resistance bacteria (eg, E. coli) that can enter the food chain in different ways, can indeed survive on foods causing disease to humans. Hence, the introduction of a rapid detection technology becomes necessary for the food industry to ensure consumer safety, especially for products with short shelf lives. Bacteriophages can be used to detect and identify bacteria. In this study, a novel biosensor is proposed to detect pathogens by means of phage‐based baroreceptor. The biosensing technique is based on millimeter‐waves technology in the 30 to 60 GHz frequency range. The proposed biosensor can detect the pathogenic bacteria in different food samples by using a diamond‐shape microstrip slot antenna. The bacteriophage‐bacterium interaction is detected through the dynamic changes in transmission lines and antennas responses. The correctness of the antenna to detect E. coli in real food sample (tomato) is also investigated. The results indicate that, through the designed sensing elements, the transient interaction between bacteria and phage can effectively be detected. This sensing mechanism allows for a faster, more accurate, and low‐cost detection of pathogenic bacteria than traditional assays. Finally, the results are compared with previously reported sensing techniques.

show abstract

Section: Resultsmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Using millimeter‐waves for rapid detection of pathogenic bacteria in food based on bacteriophage

Sultan

Ali

Fahmy

et al. 2019

Engineering Reports

View full text Add to dashboard Cite

show abstract

“…The antibody (2.5 µg/mL) addition resulted in a wavelength shift to 532 nm and decreased the absorbance peak to 1.16 (blue line). This change has indicated antibody binding to AuNPs, as reported in the literature [30]. Two pig serum samples testing positive for PCV-2 concentrations were added.…”

Section: Biosensor Developmentmentioning

confidence: 75%

A Methodology for Porcine Circovirus 2 (PCV-2) Quantification Based on Gold Nanoparticles

et al. 2020

Self Cite

View full text Add to dashboard Cite

The aim of the current study is to introduce a methodology aimed at producing a biosensor that uses gold nanoparticles (AuNPs) to detect porcine circovirus 2 (PCV-2). This biosensor was based on AuNPs, which were modified with self-assembled monolayers (SAMs) and antibodies. The AuNPs' surface and virus modification process applied to enable antibody binding was accompanied by localized surface plasmon resonance (LSPR), surface plasmon resonance (SPR), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). Virus quantification was possible by the light absorption difference in the spectrum at concentrations of 10 5 , 10 6 , 10 7 , 10 8 , and 10 9 DNA copies/mL PCV-2 in relation to quantitative PCR (qPCR), with an R 2 value >0.98. The visualization of colorimetric changes in the different PCV-2 concentrations was possible without the use of equipment. The biosensor production methodology presented reproducibility and specificity, as well as easy synthesis and low cost. An enhanced version of it may be used in the future to replace traditional tests such as PCR.

show abstract

“…Another study that utilizes the antibody functionalized AuNPs for detection of Lactobacillus spp. and Staphylococcus aureus was presented by Verdoot et al [74]. In this case the local surface plasmon resonance (LSPR) band of the AuNPs is monitored by means of UV–Vis spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

Development of nanoparticle-based optical sensors for pathogenic bacterial detection

et al. 2017

View full text Add to dashboard Cite

BackgroundPathogenic bacteria contribute to various globally important diseases, killing millions of people each year. Various fields of medicine currently benefit from or may potentially benefit from the use of nanotechnology applications, in which there is growing interest. Disease-related biomarkers can be rapidly and directly detected by nanostructures, such as nanowires, nanotubes, nanoparticles, cantilevers, microarrays, and nanoarrays, as part of an accurate process characterized by lower sample consumption and considerably higher sensitivity. There is a need for accurate techniques for pathogenic bacteria identification and detection to allow the prevention and management of pathogenic diseases and to assure food safety.ConclusionThe focus of this review is on the current nanoparticle-based techniques for pathogenic bacterial identification and detection using these applications.

show abstract

Development of a rapid and sensitive immunosensor for the detection of bacteria

Cited by 57 publications

References 14 publications

Using millimeter‐waves for rapid detection of pathogenic bacteria in food based on bacteriophage

Using millimeter‐waves for rapid detection of pathogenic bacteria in food based on bacteriophage

A Methodology for Porcine Circovirus 2 (PCV-2) Quantification Based on Gold Nanoparticles

Development of nanoparticle-based optical sensors for pathogenic bacterial detection

Contact Info

Product

Resources

About