Development of a rapid, accurate glasshouse bioassay for assessing fusarium wilt disease responses in cultivated <i>Gossypium</i> species

López-Lavalle, Luis Augusto Becerra; Potter, N. A.; Brubaker, Curt L.

doi:10.1111/j.1365-3059.2012.02603.x

Cited by 23 publications

(15 citation statements)

References 19 publications

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“…Soil bioassays in greenhouse environments have been used for screening host germplasm for resistance to various soilbome diseases, including Fusarium wilts (47). Often such bioassays entail a range of inoculum concentrations in sterilized soil or other growing media to assess host resistance (e.g., 8). When soil is the focus of evaluation, standardized methods can be used in which soil(s) are in oculated with increasing concentrations of the pathogen (2).…”

Section: Discussionmentioning

confidence: 99%

A Soil Bioassay for Predicting the Risk of Spinach Fusarium wilt

Gatch

Toit

2015

Plant Disease

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The maritime Pacific Northwest is the only region of the United States suitable for production of spinach seed, a cool-season, daylength-sensitive crop. However, the acidic soils of this region are highly conducive to spinach Fusarium wilt, caused by Fusarium oxysporum f. sp. spinaciae. Rotations of at least 10 to 15 years between spinach seed crops are necessary to reduce the high risk of losses to this disease. The objectives of this study were to develop a greenhouse soil bioassay to assess the relative risk of Fusarium wilt in fields intended for spinach seed production, and to identify soil chemical and physical properties associated with conduciveness to this disease. Preliminary bioassays established a protocol for growing spinach plants in a greenhouse environment and inducing Fusarium wilt symptoms so that the bioassay can be completed in <2 months. Test soils with a range of Fusarium wilt inoculum potentials, and three spinach inbred parent lines (highly susceptible, moderately susceptible, and moderately resistant to Fusarium wilt) were used to evaluate sensitivity of the bioassay to different levels of risk of Fusarium wilt. Then, from 2010 to 2013, spinach seed growers and stakeholders submitted soil samples from 147 fields for evaluation with the bioassay. The fields were each under consideration for planting a spinach seed crop, yet the bioassay revealed a wide range in Fusarium wilt inoculum potential among soil samples. Differences in susceptibility to Fusarium wilt of the three inbred lines were key to detecting differences in wilt risk among soils. Visits to spinach seed crops planted in fields evaluated in the bioassay, as well as test plots of the three inbred lines planted in growers’ seed crops, confirmed the predictive value of the bioassay for Fusarium wilt risk. Correlation analyses for 23 soil properties revealed significant relationships of 15 soil properties with the Fusarium wilt potential of a soil, but the correlations were influenced significantly by susceptibility of the inbred line to Fusarium wilt (13, 10, and 8 soil properties correlated significantly with Fusarium wilt risk for the susceptible, moderate, and partially resistant inbreds, respectively). Multiple regression analyses identified different statistical models for prediction of Fusarium wilt risk depending on the spinach inbred line, but the best fitting model explained <34% of the variability in Fusarium wilt risk among 121 fields evaluated in the soil bioassay. Thus, no model was robust enough to replace the bioassay for the purpose of predicting Fusarium wilt risk.

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Section: Discussionmentioning

confidence: 99%

A Soil Bioassay for Predicting the Risk of Spinach Fusarium wilt

Gatch

Toit

2015

Plant Disease

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“…Colony‐forming unit (CFU) was determined by the plate count method using serial dilutions of the mycelium suspension as reported by Becerra Lopez‐Lavalle et al. ().…”

Section: Methodsmentioning

confidence: 99%

Somatic Hybrids Between Potato and S. berthaultii Show Partial Resistance to Soil‐Borne Fungi and Potato Virus Y

Nouri-Ellouz

Triki²,

Jbir-Koubaa³

et al. 2016

Journal of Phytopathology

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Three tetraploid somatic hybrid lines produced by protoplast fusion between a dihaploid potato, Solanum tuberosum, cultivar BF15 and the wild potato species Solanum berthaultii were evaluated here for their response to different soil‐borne pathogens, that is Fusarium solani, Pythium aphanidermatum and Rhizoctonia solani as well as to infection by potato virus Y (PVY). Both hybrid and BF15 plants grown in vitro were inoculated with the tested pathogen strains, that is R. solani, P. aphanidermatum, or F. solani. The growth level and disease severity index of these plants were compared to the susceptible commercial cultivar Spunta. A better growth of inoculated hybrid plants and restricted disease symptoms were observed in comparison with the commercial plants. Under glasshouse conditions and after inoculation with R. solani and P. aphanidermatum, improved resistance of the hybrid plants to these pathogens was confirmed. Indeed, these plants showed no significant damage following inoculation and a better development in R. solani‐infected plants. The susceptibility of the hybrid tubers to R. solani, P. aphanidermatum, and to F. solani infection was also determined. A significant reduction of tissue colonisation was observed in all the hybrid lines compared to the cultivated cultivars. The STBc and STBd hybrids also showed improved resistance to the PVY ordinary strain (PVYo) under glasshouse conditions.

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“…An F 3 population consisting of 244 lines was developed from this cross, and from single seed decent from each F 3 line, 244 F 4 lines subsequently produced. The F 3 and F 4 populations were assayed for Fusarium wilt resistance using a glasshouse bioassay [52] and genotyped with 151 markers (95 SSR and 56 AFLP). QTL analysis revealed the presence of multiple regions that were associated with resistance that provide targets for introgression into elite cultivars to improve Fusarium wilt resistance [51].…”

Section: Other Cotton Germplasm Resources Beyond Seed Collections 41mentioning

confidence: 99%