Development of a Quercetin-loaded nanostructured lipid carrier formulation for topical delivery

Guo, Chenyu; Yang, Cheng‐Fu; Li, Qilu; Tan, Qi; Xi, Yang; Liu, Weina; Zhai, Guangxi

doi:10.1016/j.ijpharm.2012.03.042

Cited by 184 publications

(50 citation statements)

References 35 publications

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“…Then, these individual organ specimen was stained with hematoxylin and eosin. The excised skin and isolated organs were preserved in 10% formalin, and sections were fixed as well as blocks were made using the procedure as reported (Chen-Yu et al, 2012).…”

Section: Visualization Of the Formulation Into The Skin Penetrated Inmentioning

confidence: 99%

Nanoemulsion gel-based topical delivery of an antifungal drug:in vitroactivity andin vivoevaluation

Samad²,

et al. 2014

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Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard University, New Delhi, India AbstractObjective: In this study, attempt has been focused to prepare a nanoemulsion (NE) gel for topical delivery of amphotericin B (AmB) for enhanced as well as sustained skin permeation, in vitro antifungal activity and in vivo toxicity assessment. Materials and methods: A series of NE were prepared using sefsol-218 oil, Tween 80 and Transcutol-P by slow spontaneous titration method. Carbopol gel (0.5% w/w) was prepared containing 0.1% w/w AmB. Furthermore, NE gel (AmB-NE gel) was characterized for size, charge, pH, rheological behavior, drug release profile, skin permeability, hemolytic studies and ex vivo rat skin interaction with rat skin using differential scanning calorimeter. The drug permeability and skin irritation ability were examined with confocal laser scanning microscopy and Draize test, respectively. The in vitro antifungal activity was investigated against three fungal strains using the well agar diffusion method. Histopathological assessment was performed in rats to investigate their toxicological potential.Results and discussion: The AmB-NE gel (18.09 ± 0.6 mg/cm 2 /h) and NE (15.74 ± 0.4 mg/cm 2 /h) demonstrated the highest skin percutaneous permeation flux rate as compared to drug solution (4.59 ± 0.01 mg/cm 2 /h) suggesting better alternative to painful and nephrotoxic intravenous administration. Hemolytic and histopathological results revealed safe delivery of the drug. Based on combined results, NE and AmB-NE gel could be considered as an efficient, stable and safe carrier for enhanced and sustained topical delivery for AmB in local skin fungal infection. Conclusion: Topical delivery of AmB is suitable delivery system in NE gel carrier for skin fungal infection.

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Section: Visualization Of the Formulation Into The Skin Penetrated Inmentioning

confidence: 99%

Nanoemulsion gel-based topical delivery of an antifungal drug:in vitroactivity andin vivoevaluation

Samad²,

et al. 2014

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“…By the histopathological study, the gross anatomical changes in the major organs were observed and compared with normal anatomy. The major organs were isolated and preserved in 10% formalin solution for histopathological study as per procedure reported (Chen-Yu et al, 2012). Skin, liver, kidney and heart were studied after dissection.…”

Section: In Vivo Toxicities Evaluationmentioning

confidence: 99%

Elastic liposome-based gel for topical delivery of 5-fluorouracil: in vitro and in vivo investigation

Hussain

Samad²,

Ramzan

et al. 2014

Drug Delivery

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Objective: This investigation has focused to characterize the elastic liposome containing 5-fluorouracil (5-FU) and to enhance drug permeation across stratum corneum (SC) of the skin (rat) using various surfactants and in vivo dermal toxicity evaluation. Methodology: 5-FU-loaded elastic liposomes were developed, prepared and characterized for their entrapment efficiency, vesicle size, number of vesicles, morphological characteristics, surface charge and turbidity. In vitro drug release profile, in vitro skin permeation potential and in vitro hemolytic ability of the formulation have been evaluated to compare with drug solution for 24 h. In vitro skin permeation potential was also compared with marketed cream. Furthermore, in vivo skin irritation potential, drug penetration into the skin using confocal laser scanning microscopy (CLSM) and in vivo toxicity studies were performed. Results and conclusions:The optimized elastic liposomes demonstrated maximum drug entrapment efficiency, optimum vesicular size and considerable elasticity. In vitro skin permeation studies showed the highest drug permeation flux like 77.07 ± 6.34, 89.74 ± 8.5 and 70.90 ± 9.6 mg/cm 2 /h for EL3-S60, EL3-S80 and EL3-T80, respectively, as compared to drug solution (8.958 ± 6.9 mg/cm 2 /h) and liposome (36.80 ± 6.4 mg/cm 2 /h). Drug deposition of optimized elastic liposome EL3-S80 was about three fold higher than drug solution. Skin irritation and CLSM studies suggested that optimized gel was free from skin irritation and capable to deliver 5-FU into the epidermal area for enhanced topical delivery than drug solution. The in vitro study showed minimum hemolysis in the optimized formulation. Finally, in vivo toxicity studies followed with hisptopathological assessment showed that elastic liposome was able to extract SC to improve drug permeation without changing general anatomy of the skin.

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“…However, both systems were obtained using organic solvents and a complex, multistep preparation method [19].…”

Section: Introductionmentioning

confidence: 99%

Freeze-dried eudragit-hyaluronan multicompartment liposomes to improve the intestinal bioavailability of curcumin

Catalán-Latorre

Ravaghi

Manca

et al. 2016

European Journal of Pharmaceutics and Biopharmaceutics

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Development of a Quercetin-loaded nanostructured lipid carrier formulation for topical delivery

Cited by 184 publications

References 35 publications

Nanoemulsion gel-based topical delivery of an antifungal drug:in vitroactivity andin vivoevaluation

Nanoemulsion gel-based topical delivery of an antifungal drug:in vitroactivity andin vivoevaluation

Elastic liposome-based gel for topical delivery of 5-fluorouracil: in vitro and in vivo investigation

Freeze-dried eudragit-hyaluronan multicompartment liposomes to improve the intestinal bioavailability of curcumin

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