Development of a quantitative method for evaluating small intestinal motility          using ultrasonography in mice

Kishi, Kazuhisa; Kaji, Noriyuki; Endo, M; Tsuru, Yoshiharu; Oikawa, Tetsuro; Hori, Masaru

doi:10.1538/expanim.19-0030

Cited by 3 publications

(2 citation statements)

References 33 publications

(40 reference statements)

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“…By taking advantage of this feature, ultrasonography allows measurements at different time points in the same animal. Additionally, we previously reported that ultrasonography can quantitatively assess small intestinal motility in the longitudinal direction over time [29]. The present study shows the usefulness of a new analytical method to quantify changes in colonic motility.…”

Section: Discussionsupporting

confidence: 57%

A Novel Noninvasive Method for Quantitative Detection of Colonic Dysmotility Using Real-Time Ultrasonography

et al. 2020

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Introduction: Colonic motility disorders are a frequent clinical problem caused by various drugs and diseases. However, the etiology of colonic dysmotility is often unclear due to the lack of in vivo methods, including rapid dynamic assessment. Objectives: The aim of this study was to establish a novel quantitative method to objectively assess colonic motility using ultrasonography. Methods: We applied echocardiographic speckle tracking-based strain imaging to analyze murine colonic motility. A trace line was placed on the boundary between the proximal wall of the colon and the inner cavity to analyze colonic wall displacement and strain rate. Locomotion activities of the colonic wall were used to quantify colonic motility via ultrasonography. Results: We found that ultrasonography can quantitatively detect a decrease in colonic motility induced by loperamide, an antidiarrheal drug. These quantitative data were consistent with the imaging findings of colonic peristalsis and colon transit time. Additionally, ultrasonography also revealed changes in colonic motility over short intervals. Furthermore, we have shown that ultrasonography can quantitatively and noninvasively detect colonic dysmotility and hypervascularity of the colonic wall in colitis mice. Conclusions: These findings suggest that ultrasonography is a useful in vivo method for objectively monitoring changes in colonic motility caused by drugs and diseases.

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Section: Discussionsupporting

confidence: 57%

A Novel Noninvasive Method for Quantitative Detection of Colonic Dysmotility Using Real-Time Ultrasonography

et al. 2020

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“…Gastric emptying was evaluated by the 13 C-octanoic acid breath test as previously reported [20]. The animals were fasted for 12–16 h and placed in a chamber that was large enough for the mice to move freely.…”

Section: Methodsmentioning

confidence: 99%

Contribution of Serotonin 3A Receptor to Motor Function and Its Expression in the Gastrointestinal Tract

et al. 2020

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Introduction: The serotonin 3A receptor (5-HT3AR) is involved in vomiting and gastrointestinal motility. However, it is not well understood the expression pattern of 5-HT3AR in the gut immunohistochemically and how much contribution of 5-HT3AR to upper or lower intestinal motility. Objectives: We investigated the contribution of 5-HT3AR to gastrointestinal motor function by using 5-HT3AR KO mice and sought to identify 5-HT3AR-expressing cells via immunohistochemical staining using 5-HT3AR-GFP reporter mice. Methods: The expression of 5-HT3AR was measured in each section of the gut through real-time PCR. The motor function of the stomach and colon was assessed via the 13C-octanoic acid breath test and colonic bead expulsion test, respectively, using 5-HT3AR KO mice. 5-HT3AR-expressing cells in the muscle layer of the gut were identified by immunohistochemical staining using 5-HT3AR-GFP reporter mice. Results: 5-HT3AR was expressed throughout the digestive tract, and 5-HT3AR expression in the stomach and lower digestive tract was higher than that in the other sections. Motor function in the stomach and colon was lower in 5-HT3AR KO mice than in WT mice. As a result of immunohistochemical staining using GFP reporter mice, cholinergic neurons and PDGFRα+ cells were shown to express 5-HT3AR. In contrast, 5-HT3AR indicated by GFP fluorescence was rarely detected in ICC and smooth muscle cells. Conclusions: These results show that 5-HT3AR is highly expressed in the stomach and large intestine and that the activation of 5-HT3AR accelerates gastric emptying and large intestine transit. Additionally, 5-HT3AR is highly expressed in cholinergic neurons and some interstitial cells, such as PDGFRα+ cells.

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Innovations in noninvasive methods for measuring gastrointestinal motility in mice

Kishi

Kaji

Hori

2021

Translational and Regulatory Sciences

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Development of a quantitative method for evaluating small intestinal motility using ultrasonography in mice

Cited by 3 publications

References 33 publications

A Novel Noninvasive Method for Quantitative Detection of Colonic Dysmotility Using Real-Time Ultrasonography

A Novel Noninvasive Method for Quantitative Detection of Colonic Dysmotility Using Real-Time Ultrasonography

Contribution of Serotonin 3A Receptor to Motor Function and Its Expression in the Gastrointestinal Tract

Innovations in noninvasive methods for measuring gastrointestinal motility in mice

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