Development of a Pumpless Microfluidic System to Study the Interaction between Gut Microbes and Intestinal Epithelial Cells

Lee, Seung Yeon; Byun, Hyun Jae; Choi, Heelak; Won, Jong‐In; Han, Jeonghun; Park, Sungsu; Kim, Dong-Hyun; Sung, Jong Hwan

doi:10.1007/s12257-021-0268-x

Cited by 7 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous studies, we have shown expression of tight junction proteins Occludin and ZO‐1 by culturing Caco‐2 cells in the same (Y. Lee et al, 2021) or similar devices (S. Y. B. Lee et al, 2022). Viability of fibroblasts and HaCaT cells were examined by staining with live/dead stain.…”

Section: Resultsmentioning

confidence: 99%

Multiorgan‐on‐a‐chip for realization of gut‐skin axis

Lee

Sung

2022

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

The concept of physiological link between the gut and the skin, known as the gutskin axis, has been gaining more evidence recently. Although experimental data from animal and human studies support the existence of the gut-skin axis, in vitro model platforms that can test the hypothesis are lacking. Organ-on-a-chip offers the possibility of connecting different tissues and recapitulating interactions between them. In this study, we report a multiorgan chip that can capture the basic interorgan communication between the gut and the skin. Its modular design enables separate culture and differentiation of the gut and skin tissues, and after assembly the two organs are connected via microfluidic channels than enables perfusion and mass transfer. We showed that the impairment of the gut barrier function exacerbated the adverse effect of fatty acids on skin cells, with decreased viability, increased level of cytokine secretion and human beta defensin-2 (hBD-2), an inflammatory dermal disease marker. Based on these results, we believe that our multiorgan chip can be a novel in vitro platform for recapitulating complex mechanisms underlying the gutskin axis.

show abstract

Section: Resultsmentioning

confidence: 99%

Multiorgan‐on‐a‐chip for realization of gut‐skin axis

Lee

Sung

2022

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although the concept of pumpless design is not new and has been explored in prior literature, it is important to note that past approaches have often encountered limitations in terms of throughput or operational convenience, making them less practical for industrial use. [45][46][47][48][49][50][51] In addition to providing compatibility with automated liquid handling devices, our chip design also permits multiple chips to be stacked and operated at the same time, significantly increasing the throughput and efficiency of experiments. Researchers can simultaneously investigate multiple experimental conditions, accelerating the pace of discovery and development.…”

Section: Discussionmentioning

confidence: 99%

A generic pump‐free organ‐on‐a‐chip platform for assessment of intestinal drug absorption

Guo,

Xie,

Qin

2024

Biotechnology Journal

View full text Add to dashboard Cite

Organ‐on‐a‐chip technology has shown great potential in disease modeling and drug evaluation. However, traditional organ‐on‐a‐chip devices are mostly pump‐dependent with low throughput, which makes it difficult to leverage their advantages. In this study, we have developed a generic, pump‐free organ‐on‐a‐chip platform consisting of a 32‐unit chip and an adjustable rocker, facilitating high‐throughput dynamic cell culture with straightforward operation. By utilizing the rocker to induce periodic fluid forces, we can achieve fluidic conditions similar to those obtained with traditional pump‐based systems. Through constructing a gut‐on‐a‐chip model, we observed remarkable enhancements in the expression of barrier‐associated proteins and the spatial distribution of differentiated intestinal cells compared to static culture. Furthermore, RNA sequencing analysis unveiled enriched pathways associated with cell proliferation, lipid transport, and drug metabolism, indicating the ability of the platform to mimic critical physiological processes. Additionally, we tested seven drugs that represent a range of high, medium, and low in vivo permeability using this model and found a strong correlation between their Papp values and human Fa, demonstrating the capability of this model for drug absorption evaluation. Our findings highlight the potential of this pump‐free organ‐on‐a‐chip platform as a valuable tool for advancing drug development and enabling personalized medicine.

show abstract

“…More importantly, coculturing a single strain of bacteria, F. Prausnitzii , extensively altered the gene expression patterns related to inflammation and Toll-like receptor (TLR) signaling, supporting the anti-inflammatory effect of F. Prausnitzii . Finally, a pumpless microfluidic model system was also developed using gravity-driven flow for coculturing gut bacteria with human colon epithelial cells in an oxygen gradient [ 76 ]. The oxygen gradient was established by placing the culture device in the anaerobic environment while flowing an oxygenated medium located in an aerobic condition through the tubing.…”

Section: Current Developments Of Organotypic Coculture Model Systemsmentioning

confidence: 99%

“…1,2 Bacterial loads in the human adult intestines and other organs are referenced from [ 20 ] and [ 111 ], respectively. 3–21 Images are reproduced, respectively, from [ 13 , 34 , 41 , 42 , 68 , 69 , 72 , 74 – 76 , 78 , 79 , 87 , 88 , 98 , 101 , 102 , 114 , 116 ] with the permission of publishers. The image was created with BioRender.com …”

Section: Current Developments Of Organotypic Coculture Model Systemsmentioning

confidence: 99%

Advanced Organotypic In Vitro Model Systems for Host–Microbial Coculture

2023

BioChip J

View full text Add to dashboard Cite

In vitro model systems have been advanced to recapitulate important physiological features of the target organ in vivo more closely than the conventional cell line cultures on a petri dish. The advanced organotypic model systems can be used as a complementary or alternative tool for various testing and screening. Numerous data from germ-free animal studies and genome sequencings of clinical samples indicate that human microbiota is an essential part of the human body, but current in vitro model systems rarely include them, which can be one of the reasons for the discrepancy in the tissue phenotypes and outcome of therapeutic intervention between in vivo and in vitro tissues. A coculture model system with appropriate microbes and host cells may have great potential to bridge the gap between the in vitro model and the in vivo counterpart. However, successfully integrating two species in one system introduces new variables to consider and poses new challenges to overcome. This review aims to provide perspectives on the important factors that should be considered for developing organotypic bacterial coculture models. Recent advances in various organotypic bacterial coculture models are highlighted. Finally, challenges and opportunities in developing organotypic microbial coculture models are also discussed.

show abstract

Development of a Pumpless Microfluidic System to Study the Interaction between Gut Microbes and Intestinal Epithelial Cells

Cited by 7 publications

References 39 publications

Multiorgan‐on‐a‐chip for realization of gut‐skin axis

Multiorgan‐on‐a‐chip for realization of gut‐skin axis

A generic pump‐free organ‐on‐a‐chip platform for assessment of intestinal drug absorption

Advanced Organotypic In Vitro Model Systems for Host–Microbial Coculture

Contact Info

Product

Resources

About