Development of a Protocol for the Quantitative Evaluation of Contrast in NMR Images of Cryoprotective Solvents in Intact Tissues

Isbell, Stephanie A.

doi:10.1006/cryo.1996.1995

Cited by 6 publications

(3 citation statements)

References 4 publications

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“…Different methods have been developed to measure CPA diffusivity in tissues [1,2,6,10,24]. In organs, even as simple as mouse ovaries, there exist different types of tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Magnetic resonance imaging (MRI) is a widely used technique to detect spatial distribution of chemical components inside 3D structures without invasive methods [5,10]. Different MRI techniques have been developed to investigate CPA perfusion processes in tissues.…”

Section: Introductionmentioning

confidence: 99%

“…In this study we proposed the use of a phenomenological mass transfer property of CPA: the apparent CPA diffusivity, D̄ . Instead of measuring the CPA gradient inside tissues or MR spectrum frequency shift as previously described [10,14], we calculated the average CPA concentration in the centric cross-section (the cross-section with maximum area) of mouse ovaries from the MRI images and fit these data to the integral form of the mass transfer equations to obtain D̄ . With this phenomenological parameterization the requirement of image quality is lowered, and the characteristics of the EG perfusion processes in mouse ovaries can be investigated and used to optimize CPA perfusion procedures.…”

Section: Introductionmentioning

confidence: 99%

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Measurement of the apparent diffusivity of ethylene glycol in mouse ovaries through rapid MRI and theoretical investigation of cryoprotectant perfusion procedures

Han¹,

Ma²,

Benson³

et al. 2009

Cryobiology

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Successful organ cryopreservation will significantly benefit human health and biomedical research. One of the major challenges to this accomplishment is the need for optimization of cryoprotectant agent (CPA) perfusion procedures that involve highly complicated mass transfer processes in organs. The diffusivity of CPA is of critical importance for designing perfusion procedures to minimize the associated toxicity and osmotic damage. However, to date there have been no attempts to measure the CPA diffusivity in organs. In this study, we established a simple CPA diffusion model for relatively small organs, e.g. mouse ovaries, defined the apparent diffusivity (D) of CPA for these organs (please see Table 1 for symbol definitions), and established a practical approach to measure the value of D̄ through magnetic resonant imaging (MRI). Using rapid MRI techniques and water saturation analyses, the distribution of ethylene glycol (EG) concentration in the centric cross-section of mouse ovaries was measured at a series of time points during perfusion, and these data were fit to the integral form of the mass transfer equation in the established model. These fits resulted in a value of D̄ for EG in mouse ovaries of 6.1 ± 1.4×10 -7 cm 2 /s (mean ± SD). Based on these results, we proposed a modified perfusion procedure that may improve the survival of small organs or thin tissues during equilibrium cooling processes and assessed its efficiency through theoretical analyses.

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“…Different methods have been developed to measure CPA diffusivity in tissues [1,2,6,10,24]. In organs, even as simple as mouse ovaries, there exist different types of tissues.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%