Development of a pronuclear DNA microinjection technique for production of green fluorescent protein-expressing bubaline (Bubalus bubalis) embryos

Verma, Vinod; Gautam, Sanjeev K.; Palta, P.; Manik, R. S.; Singla, S. K.; Chauhan, M. S.

doi:10.1016/j.theriogenology.2007.09.035

Cited by 6 publications

(2 citation statements)

References 34 publications

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“…Transgenic animals are used for producing animals’ models for disease resistance, mastitis resistant and for improved quantitative and qualitative traits [ 82 ]. Another use of transgenic farm animals is as bioreactors for producing human recombinant proteins in mammary gland [ 83 , 84 ]. As bioreactors, transgenic animals can be designed to produce milk of human growth hormone, high casein content, and human hemoglobin, anti-thrombin III and anti-tripsin in milk [ 82 ] and higher production of wool.…”

Section: Stem Cell Technologymentioning

confidence: 99%

Advances in reproductive biotechnologies

Choudhary¹,

Kavya²,

Adnot³

et al. 2016

Vet World

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In recent times, reproductive biotechnologies have emerged and started to replace the conventional techniques. It is noteworthy that for sustained livestock productivity, it is imperative to start using these techniques for facing the increasing challenges for productivity, reproduction and health with impending environment conditions. These recent biotechniques, both in male and female, have revolutionized and opened avenues for studying and manipulating the reproductive process both in vitro and in vivo in various livestock species for improving tis efficiency. This review attempts to highlight pros and cons, on the recent developments in reproductive biotechnologies, both in male and female in livestock species.

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Section: Stem Cell Technologymentioning

confidence: 99%

Advances in reproductive biotechnologies

Choudhary¹,

Kavya²,

Adnot³

et al. 2016

Vet World

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“…In addition, fluorescent reporter genes have opened new vistas for embryology and stem cell research and therefore could be used as reporters for the activity of gene promoters tagged to functional proteins to study their intracellular localisation in living cells, tissues and organisms (Habermann et al 2007). A pronuclear microinjection method has been developed for the production of transgenic buffalo embryos expressing green fluorescent protein (GFP) and embryos with GFP expression at different developmental stages have been obtained (Verma et al 2008).…”

Section: Stem Cell Technology and Transgenesismentioning

confidence: 99%

Reproductive biotechniques in buffaloes (Bubalus bubalis): status, prospects and challenges

Singh

Chauhan

Singla

et al. 2009

Reprod. Fertil. Dev.

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The swamp buffalo holds tremendous potential in the livestock sector in Asian and Mediterranean countries. Current needs are the faster multiplication of superior genotypes and the conservation of endangered buffalo breeds. Recent advances in assisted reproductive technologies, including in vitro embryo production methodologies, offer enormous opportunities to not only improve productivity, but also to use buffaloes to produce novel products for applications to human health and nutrition. The use of molecular genomics will undoubtedly advance these technologies for their large-scale application and resolve the key problems currently associated with advanced reproductive techniques, such as animal cloning, stem cell technology and transgenesis. Preliminary success in the application of modern reproductive technologies warrants further research at the cellular and molecular levels before their commercial exploitation in buffalo breeding programmes.

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