Development of a point-of-care assay system for high-sensitivity C-reactive protein in whole blood

Ahn, Jae Soon; Choi, Sunga; Jang, Sang Ho; Chang, Hyuk Jae; Kim, Jae-Hoon; Nahm, Ki Bong; Oh, Sang Wook; Choi, Eui Yul

doi:10.1016/s0009-8981(03)00113-x

Cited by 93 publications

(47 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Approaches using other physical properties of the label include the localised surface plasmon resonance of the gold nanoparticles to increase the signal [66] and surfaceenhanced Raman scattering of gold or silver nanoparticles [45]. More sensitive chemiluminescent [50] or fluorescent [24,47,48,92] labels may increase the sensitivity, but will increase the cost of the assay, because more sophisticated hardware and software are needed to read the signal. A typical approach that is used for signal enhancement and quantitation employs hexacyanoferrate(II)-loaded liposomes in combination with a melittin-labelled analyte analogue [80].…”

Section: Weaknessesmentioning

confidence: 99%

See 1 more Smart Citation

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

2008

View full text Add to dashboard Cite

Lateral flow (immuno)assays are currently used for qualitative, semiquantitative and to some extent quantitative monitoring in resource-poor or non-laboratory environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quantitative monitoring. We illustrate the potentials and limitations of analysis with lateral flow (immuno)assays using a literature survey and a SWOT analysis (acronym for "strengths, weaknesses, opportunities, threats"). Articles referred to in this survey were searched for on MEDLINE, Scopus and in references of reviewed papers. Search terms included "immunochromatography", "sol particle immunoassay", "lateral flow immunoassay" and "dipstick assay".

show abstract

Section: Weaknessesmentioning

confidence: 99%

“…One approach is implemented by, e. g., Biodot, and is described in [94]. The use of an internal standard for higher reproducibility was mentioned [47,48].…”

Section: Opportunitiesmentioning

confidence: 99%

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

2008

View full text Add to dashboard Cite

show abstract

“…The NIR-LFA, however, has distinct advantages on several critical points. First, whereas the fluorescent assays reported have emission/ excitation spectrums ranging from UV to 720 nm, with most in the 500-to 600-nm range (3,4,(23)(24)(25)(27)(28)(29), the NIR-LFA uses dye with excitation/emission peaks of 785/820 nm. Use of a dye in the NIR region helps increase the signal-to-noise ratio in plasma and blood by reducing the background from autofluorescent proteins and matrix materials and avoids possible dampening mediated by hemoglobin absorption.…”

Section: Discussionmentioning

confidence: 99%

Lateral Flow Assay with Near-Infrared Dye for Multiplex Detection

Swanson

D’Andrea

2013

Clinical Chemistry

View full text Add to dashboard Cite

BACKGROUND Lateral flow assays (LFAs) are popular point-of-care diagnostic tools because they are rapid and easy to use. Nevertheless, they often lack analytical sensitivity and quantitative output and may be difficult to multiplex, limiting their usefulness in biomarker measurement. As a proof-of-concept study, we detail the design of a quantitative, multiplex LFA with readily available near-infrared (NIR) detection to improve analytical sensitivity. METHODS NIR dye was conjugated to selected antibodies and incorporated into LFAs. We used singleplex, optimized NIR-LFAs to measure interleukin (IL)-6 from 0 to 200 pg/mL and developed duplex assays to simultaneously measure IL-6 from 0 to 100 pg/mL (0 to 4.5 pmol/L) and C-reactive protein (CRP) from 50 to 2500 ng/mL (0.4 to 20 nmol/L) on a single test strip. Assays were tested on 60 different spiked samples and compared to ELISA results. RESULTS NIR-LFAs detected IL-6 in a 10% plasma matrix with a limit of detection of 4 pg/mL (182 fmol/L) and a CV <7%. Duplex NIR-LFAs quantitatively measured IL-6 and CRP concentrations simultaneously. Values strongly correlated to ELISA measurements, with R2 values of 0.9825 and 0.9711 for IL-6 and CRP, respectively. CONCLUSIONS NIR-LFAs exhibit quantitative measurement at pg/mL concentrations owing to a high signal-to-BACKGROUND ratio and robust detection antibody clearance through the test strip. Moreover, NIR-LFAs are able to detect molecules present at vastly different concentrations in multiplex format and compare favorably to ELISAs. LFAs with direct NIR detection may be a valuable tool for biomarker evaluation in the point-of-care setting.

show abstract

“…20,21 These new assays have improved the sensitivity and precision for low levels of CRP. 22 In the present work, we introduce QDs as fluorescent probes and developed a fluorescence POCT assay (QF-POCT) that can rapidly, sensitively, and quantitatively analyze levels of CRP and high-sensitivity CRP.…”

Section: Introductionmentioning

confidence: 99%

Rapid and quantitative detection of C-reactive protein using quantum dots and immunochromatographic test strips

Cheng¹,

Xu²,

Jun³

et al. 2014

IJN

View full text Add to dashboard Cite

Background Rapid immunochromatographic tests can detect disease markers in 10–15 minutes, which facilitates clinical diagnosis and treatment programs. However, most immunochromatographic tests employ gold nanoparticles as reporters, and these have only moderate sensitivity and act as qualitative methods for analyzing high biomarker concentrations. Methods In this study, we introduce quantum dots (QDs) as fluorescent probes and immunochromatographic strips to develop quantitative fluorescence point-of-care tests (QF-POCT) to analyze C-reactive protein (CRP) levels. Goat anti-rabbit IgG and rabbit IgG were used as control antibodies, and mouse monoclonal CRP antibody pairs were used for disease marker detection. One monoclonal CRP antibody was conjugated with QDs and served as a signal antibody, and the other monoclonal CRP antibody was dispensed onto the nitrocellulose membrane and served as a capturing antibody. In the presence of CRP, the fluorescence intensity of the monoclonal antibody-CRP-monoclonal antibody sandwich complex captured on the nitrocellulose membrane was determined using the fluorescence strip reader. Results QF-POCT assays could quantitatively analyze the concentration of CRP in 15 minutes had a detection limit of 0.25 mg/L, and had a wide detection linearity range (0.5–300 mg/L). The intra-assay and interassay coefficients of variation were 8.95% and 9.86% at 0.5 mg/L, 6.47% and 8.66% at 10 mg/L, and 6.81% and 9.10% at 60 mg/L, respectively. In a comparison between clinical samples, the results of this QD-based assay of CRP levels were significantly correlated with those of an Immulite 2000 assay ( R =0.993, P <0.001). Conclusion Our results demonstrated that the QD-based immunochromatographic test is a rapid, sensitive, accurate, and quantitative method for the detection of disease biomarkers.

show abstract

Development of a point-of-care assay system for high-sensitivity C-reactive protein in whole blood

Cited by 93 publications

References 25 publications

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

Lateral Flow Assay with Near-Infrared Dye for Multiplex Detection

Rapid and quantitative detection of C-reactive protein using quantum dots and immunochromatographic test strips

Contact Info

Product

Resources

About