Development of a planar chromatographic method for quantitation of anthocyanes in pomace, feed, juice and wine

Analysis of anthocyanins in powdered berry extracts by planar chromatography linked with bioassay and mass spectrometry

“…Calibrations were listed for the compounds, 248 which were found and quantified in the berry samples. The calibration performance for all 249 anthocyanins, especially for the five anthocyanidins, were recently reported with 250 correlation coefficients ≥ 0.9993(Krüger, Urmann, & Morlock, 2013). 251…”

“…173separation of anthocyanins(Krüger, Urmann, & Morlock, 2013). The plate activity was adjusted at a relative air humidity of 25 ± 2 % for 4 min using a saturated potassium acetate 175 solution (260 g/100 g water) in the humidity control unit.…”

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

“…14 The detection of bioactive compounds in complex samples by bacterial assays using the directly bioluminescent marine bacteria Vibrio fischeri has been reported. [15][16][17] Many enzyme substrate reactions that show the viability of microorganism are suited for use in HPTLC bioassay. For instance, in the detection of algicides 18 and fungicides.…”

Analysis of Bioactive Components of Oilseed Cakes by High-Performance Thin-Layer Chromatography-(Bio)assay Combined with Mass Spectrometry

“…The Aliivibrio fischeri bioassay was performed according to previous studies [13]. Briefly, the developed, neutralized and dried plate was immersed into a A. fischeri suspension that was prepared according to DIN EN ISO 11348-1, Section 5 [20] for 2 s at an immersion speed of 2 cm/s.…”

“…HPTLC has advantages, such as simplified sample preparation, low operating costs, short analysis time and simultaneous analysis of several samples. HPTLC has been employed in the study of anthocyanins and bioactivities of extracts from grape pomace, wine, fruit juices, bilberries, cranberries, and blueberries powders [13,14]. In addition, there are several HPTLC reports on the determination of polyphenols and their bioactivities from plant materials, such as Citrullus lanatus (Thunb.)…”