Development of a Personal Bioaerosol Sampler Based on a Conical Cyclone with Recirculating Liquid Film

Tolchinsky, Alexander D.; Sigaev, Vladimir I.; Sigaev, Genneday I.; Varfolomeev, A. N.; Zvyagina, E.V.; Brasel, Trevor; Cheng, Yung Sung

doi:10.1080/15459620903486768

Cited by 11 publications

(7 citation statements)

References 10 publications

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“…A variety of samplers have been developed in collecting bioaerosols (Tolchinsky et al 2010;Haatainen et al 2010;Bundke et al 2010;Park et al 2009;Gorner et al 2006;Chen et al 2004). Recently, electrostatic sampling techniques for bioaerosols have also increasingly been investigated (Han et al 2010;Yao et al 2009a;Han and Mainelis 2008;Madsen and Sharma 2008;Yao and Mainelis 2006a;Mainelis et al 1999Mainelis et al , 2002a.…”

Section: Introductionmentioning

confidence: 99%

A novel method for measuring the charge distribution of airborne microbes

2010

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A novel method for measuring bioaerosol charge distribution was investigated using electrostatic sampling and quantitative polymerase chain reaction (qPCR). Here, Bacillus subtilis var niger and Pseudomonas fluorescens were aerosolized and precipitated into different regions of two 96-well plates placed inside an electrostatic sampler. The concentrations of negatively charged bacteria in the air samples collected were quantified using qPCR, and the relevant bioaerosol charge levels were calculated using an equation developed in this study. Experimental results showed that B. subtilis var niger bioaerosols had wider charge distribution than P. fluorescens. For B. subtilis var niger, 93% of them carried a charge level of 13-42 units, 2% carried a charge level of 8-13 or lower, and 5% carried a charge level of 42-195 or higher. While for P. fluorescens, 99% of P. fluorescens aerosols carried a charge level of 14-34 elementary units. The indicative charge levels of bioaerosols collected at a certain point in the electrostatic sampling line largely depends on the particle size, bacterial species, electrostatic field strength, and the sampling flow rates for a given electrostatic sampler. In general, the trends for the charge levels here were similar to those obtained in previous studies. Use of qPCR technology in this study can distinguish between biological and non-biological particles collected. Thus, this avoided counting the non-biological particles in traditional bioaerosol charge measurements in which an optical particle counter is used. The developed technique here can be also used to analyze the charge levels for other types of bioaerosols.

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Section: Introductionmentioning

confidence: 99%

A novel method for measuring the charge distribution of airborne microbes

2010

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“…The collection efficiency was used in our previous studies as an important index to evaluate the performance of the prototypes of the two test samplers (Sigaev et al, 2006;Tolchinsky et al, 2010). The capture efficiency (CP) is defined in this study as the ratio of the aerosols captured by the recirculating liquid to those entering the sampler.…”

Section: Methodsmentioning

confidence: 99%

“…Collecting airborne microorganism into a liquid medium such as physiological saline has the benefits for bioaerosol sampling not only in reducing the stress and desiccation imposed upon the bioaerosols during the sampling process (Jensen et al, 1992), but also by offering convenience to the consequent microbiological analysis such as the traditional cell culture growth analysis and the advanced polymerase chain reaction assay (Lin et al, 2000). Therefore, researchers worldwide have designed various wet-type bioaerosol samplers to facilitate optimal bioaerosol sampling, and many of them have been sold on the market or presented to the academia, such as AGI-30, GCS, BioSampler, NIOSH onestage, NIOSH two-stage, CIP-10M, and the predecessors of the test samplers in this study (Chen et al, 2004;Gorner et al, 2006;Grinshpun et al, 1994;Lindsley et al, 2006;Tolchinsky et al, 2010;Upton et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…Bioaerosol sampling into liquid using swirling air in a cyclone features high microbial viability for the collected bioaerosols because the sampling air that entered the cyclone contacts the recirculated liquid film in a tangential way, which allows the bioaerosols to be gently captured by the collection liquid (Lin et al, 1999;Tolchinsky et al, 2010). The performance evaluation of the prototype demonstrated that this cyclone-based wet-type personal bioaerosol sampler has a high aerosol collection efficiency with cutoff diameter, d 50 , equal to 0.7-0.75 mm (cutoff diameter: most of the particles greater than d 50 are collected and most of the particles less than d 50 pass through the sampler) and can permit fair bioaerosol survival (Tolchinsky et al, 2010). Recently, two improved versions were developed by adding more features on the previous prototype.…”

Section: Introductionmentioning

confidence: 99%

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A wind tunnel test of newly developed personal bioaerosol samplers

Tolchinsky²,

Sigaev³

et al. 2012

Journal of the Air & Waste Management Association

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In this study the performance of two newly developed personal bioaerosol samplers was evaluated. The two test samplers are cyclone-based personal samplers that incorporate a recirculating liquid film. The performance evaluations focused on the physical efficiencies that a personal bioaerosol sampler could provide, including aspiration, collection, and capture efficiencies. The evaluation tests were carried out in a wind tunnel, and the test personal samplers were mounted on the chest of a full-size manikin placed in the test chamber of the wind tunnel. Monodisperse fluorescent aerosols ranging from 0.5 to 20 microm were used to challenge the samplers. Two wind speeds of 0.5 and 2.0 m/sec were employed as the test wind speeds in this study. The test results indicated that the aspiration efficiency of the two test samplers closely agreed with the ACGIH inhalable convention within the size range of the test aerosols. The aspiration efficiency was found to be independent of the sampling orientation. The collection efficiency acquired from these two samplers showed that the 50% cutoff diameters were both around 0.6 microm. However the wall loss of these two test samplers increased as the aerosol size increased, and the wall loss of PAS-4 was considerably higher than that of PAS-5, especially in the aerosol size larger than 5 microm, which resulted in PAS-4 having a relatively lower capture efficiency than PAS-5. Overall, the PAS-5 is considered a better personal bioaerosol sampler than the PAS-4.

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“…Such systems achieve high sampling performance and enable the concentration of samples due to the high flow rate ratio between the incoming air and drainage liquid. Considerable research has focused on the development of wet-cyclone bioaerosol samplers [26][27][28][29][30]; however, these have a large particle cut-off diameter, and a low particle collection efficiency and aerosol-to-liquid transfer rate; furthermore, the twophase flow operation is unstable and few fully integrated bioaerosol sampling systems are capable of continuous real-time sampling.…”

Section: Introductionmentioning

confidence: 99%

Development of an automated wet-cyclone system for rapid, continuous and enriched bioaerosol sampling and its application to real-time detection

Cho

Hong

Choi

et al. 2019

Sensors and Actuators B: Chemical

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A B S T R A C TWe present a novel bioaerosol sampling system based on a wet-cyclone for real-time and continuous monitoring of airborne microorganisms. The Automated and Real-time Bioaerosol Sampler based on Wet-cyclone (ARBSW) continuously collects bioaerosols in a liquid medium and delivers the samples to a sensing device using a wireless remote control system. Based on a high air-to-liquid-flow-rate ratio (∼ 1.4 × 10 5 ) and a stable liquid thin film within a wet-cyclone, the system achieved excellent sampling performance as indicated by the high concentration and viability of bioaerosols (> 95% collection efficiency for > 0.5-μm-diameter particles, > 95% biological collection efficiency for Staphylococcus epidermidis and Micrococcus luteus). Furthermore, the continuous and real-time sampling performance of the ARBSW system under test-bed conditions and during a field test demonstrated that the ARBSW is capable of continuously monitoring bioaerosols in real time with high sensitivity. Therefore, the ARBSW shows promise for continuous real-time monitoring of bioaerosols and will facilitate the management of bioaerosol-related health and environmental issues.

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Development of a Personal Bioaerosol Sampler Based on a Conical Cyclone with Recirculating Liquid Film

Cited by 11 publications

References 10 publications

A novel method for measuring the charge distribution of airborne microbes

A novel method for measuring the charge distribution of airborne microbes

A wind tunnel test of newly developed personal bioaerosol samplers

Development of an automated wet-cyclone system for rapid, continuous and enriched bioaerosol sampling and its application to real-time detection

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