Development of a PCR assay for the detection of human herpes virus type 7

Mardanly, S S; Mardanly, S. G.; Kazakov, A. A.; Демкин, В. В.; Затевалов, А. М.; Mironov, A Yu

doi:10.51620/0869-2084-2022-67-11-658-662

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…The main method to estimate the quantity of virus number is based on virus in ity, or titer, which is time-consuming and subjective. Some researchers [37] use tra sion electron microscopy (TEM) to quickly observe viruses, but this method is exp In other studies [38], quantitative real-time polymerase chain reaction (PCR) was u achieve rapid detection of the virus, but this measured all the genetic material pre the sample, and many virus genomes were not assembled into virus particles, so tection results were not accurate. In this study, five Omicron strains were inactivate the strain with the highest S protein content was screened by Western blot.…”

Section: Discussionmentioning

confidence: 99%

Screening of Omicron Virus Strain by Quantifying the Spike Protein Content

He,

Chang,

Wang

et al. 2024

COVID

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In the development of an inactivated virus vaccine, the isolation of a single virus strain plays a key role in determining potency. The conventional methods of quantification of virus number are mainly based on virus titers, which are subjective and time-consuming, especially in the early stage of virus isolation, in which the titer difference is weak. Previous reports have shown a high correlation between the spike protein and the potency of COVID-19 vaccines. In this paper, we report a novel, fast, and convenient method of screening an Omicron virus strain by quantifying the spike protein content, where the isolated strain shows high affinity to Omicron-specific antibodies and a high titer, and it can induce high levels of neutralization antibodies.

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Section: Discussionmentioning

confidence: 99%

Screening of Omicron Virus Strain by Quantifying the Spike Protein Content

He,

Chang,

Wang

et al. 2024

COVID

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“…При изучении структуры инфекционного синдрома в качестве наиболее значимых и часто детектируемых этиологических факторов определены бактериальные и вирусные патогены (моно-и коинфекции), что соответствует данным современной литературы [21]. Локализация опосредуемого ими инфекционно-воспалительного процесса весьма разнообразна, но у преобладающего числа пациентов диагностированы инфекционно-воспалительные заболевания и поражение органов респираторного Рис.…”

Section: методы регистрации исходовunclassified

Atypical chronic active herpesvirus infections: Etiological structure, frequency of occurrence, clinical syndromes associated with them

Khalturina

Mironov

Суранова

2023

Epidemiology and Infectious Diseases

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Background: Every year, a steady, progressive increase in the number of atypical, chronic, active forms of infections caused by herpesviruses (AHA-HVI) is recorded. The diagnosis and selection of adequate therapeutic strategies for the treatment of these forms of infections presents significant difficulties for physicians of therapeutic, neurological profile, immunologists, infectious disease specialists, due to the polysyndromicity and the presence of many clinical masks of these infections. Aims: determining the prevalence of AHA-HVI among patients infected with herpes virus infections, as well as studying the features of the etiological structure and clinical manifestations/criterion signs of atypically occurring chronic forms of herpes viral infections. Materials and methods: Under our supervision at the Medsi CDC in Belorusskaya (Moscow) there were 98 patients (SG) of both sexes aged 23 to 60 years suffering from AHA-HVI. The comparison group consisted of 30 conditionally healthy subjects comparable in sex and age to SG patients. In addition to traditional methods (history collection, physical examination methods, CBC, etc.), serodiagnosis methods with ELISA were used to detect herpes-viral infections; PCR method for detecting the genome of viruses in biomaterials. The study was approved by the ethics committee, and all patients received informed consent to participate in the study. Statistical analysis was performed using adequate methods of statistic studies. Results: The study of the etiological structure of herpes virus infections in patients with AHA-HVI, mixed HVI was shown to occur in 83.4% of patients, and mono-HVI in 16.6% of cases. It was shown that EBV was the dominant virus among patients with both mono and mixed GVI. A high rate of EBV DNA detection was demonstrated in saliva (84.2%), posterior pharyngeal wall scraping (73.5%) and tonsils (42.9%), urine (12.6%) and blood (8.3%) is a marker of high replicative activity of the virus. The main clinical syndromes associated with the course of mono and mixed AHA-HVI were identified. Conclusions: Identification and quantitative assessment of the viral load associated with the severity of the course and severity of clinical manifestations of ACA-HVI, as well as clarification of the features of clinical manifestations and syndromes in patients suffering from various mono-, mixed-herpesvirus infections, allow us to outline goals for the further development of an adequate diagnostic algorithm for these atypical forms of herpesvirus infections and the concept of targeted, personalized etio- and immunopathogenetic therapy.

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Development of a PCR assay for the detection of human herpes virus type 7

Cited by 2 publications

References 10 publications

Screening of Omicron Virus Strain by Quantifying the Spike Protein Content

Screening of Omicron Virus Strain by Quantifying the Spike Protein Content

Atypical chronic active herpesvirus infections: Etiological structure, frequency of occurrence, clinical syndromes associated with them

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