Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections

Akalın, Pınar; Yazgan-Karataş, Ayten

doi:10.1016/j.mex.2023.102372

Cited by 4 publications

(5 citation statements)

References 23 publications

(26 reference statements)

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“…This research has focused on developing singleplex LAMP-LFA methods, offering streamlined and user-friendly approaches for detecting the influenza virus. Recently, Akalına and Yazgan-Karataş took a significant step forward by developing a multiplex LAMP-LFA targeting both SARS-CoV-2 and influenza [37], broadening the scope of pathogens that can be detected simultaneously. Indeed, LAMP assays typically consist of 4-6 primers per target gene, and diagnosing more than two targets simultaneously requires at least 8-12 primers, potentially leading to a high incidence of non-specific reactions [38,39].…”

Section: Discussionmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification and Lateral Flow Immunochromatography Technology for Rapid Diagnosis of Influenza A/B

Jang,

Lee,

Lee

et al. 2024

Diagnostics

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Influenza viruses cause highly contagious respiratory diseases that cause millions of deaths worldwide. Rapid detection of influenza viruses is essential for accurate diagnosis and the initiation of appropriate treatment. We developed a loop-mediated isothermal amplification and lateral flow assay (LAMP-LFA) capable of simultaneously detecting influenza A and influenza B. Primer sets for influenza A and influenza B were designed to target conserved regions of segment 7 and the nucleoprotein gene, respectively. Optimized through various primer set ratios, the assay operated at 62 °C for 30 min. For a total of 243 (85 influenza A positive, 58 influenza B positive and 100 negative) nasopharyngeal swab samples, the performance of the influenza A/B multiplex LAMP-LFA was compared with that of the commercial AllplexTM Respiratory Panel 1 assay (Seegene, Seoul, Korea). The influenza A/B multiplex LAMP-LFA demonstrated a specificity of 98% for the non-infected clinical samples, along with sensitivities of 94.1% for the influenza A clinical samples and 96.6% for the influenza B clinical samples, respectively. The influenza A/B multiplex LAMP-LFA showed high sensitivity and specificity, indicating that it is reliable for use in a low-resource environment.

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Section: Discussionmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification and Lateral Flow Immunochromatography Technology for Rapid Diagnosis of Influenza A/B

Jang,

Lee,

Lee

et al. 2024

Diagnostics

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“…In terms of the utility of AuNP in diagnostics, recent studies detail detection systems, primarily for human pathogens, that integrate RT-LAMP and lateral flow biosensors with AuNPs (Zhu et al 2020 ; Chen et al 2021 ; Akalin and Yazgan-Karatas 2023 ). However, the combination of non-functionalized AuNPs and biosensors based on fluorescently labeled LAMP primers (Zhu et al 2020 ; Chen et al 2021 ) may result in a colorimetric detection of decreased specificity.…”

Section: Discussionmentioning

confidence: 99%

An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus

Cea-Callejo,

Arca-Lafuente,

Gomez-Lucia

et al. 2024

Appl Microbiol Biotechnol

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Airborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have developed a molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys and chickens. For this purpose, a colorimetric system was obtained by coupling the LAMP technique with specific DNA-functionalized AuNPs (gold nanoparticles). The system was validated using 50 different samples (pharyngeal swabs and tracheal tissue) collected from aMPV-infected and non-infected chickens and turkeys. Viral detection can be achieved in about 60 min with the naked eye, with 100% specificity and 87.88% sensitivity for aMPV. In summary, this novel molecular detection system allows suitable virus testing in the field, with accuracy and limit of detection (LOD) values highly close to qRT-PCR-based diagnosis. Furthermore, this system can be easily scalable to a platform for the detection of other viruses, addressing the current gap in the availability of POC tests for viral detection in poultry farming. Key points •aMPV diagnosis using RT-LAMP is achieved with high sensitivity and specificity. •Fifty field samples have been visualized using DNA-nanoprobe validation. •The developed system is a reliable, fast, and cost-effective option for POCT. Graphical Abstract

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“…92 This architecture eliminates the need for specific optimization of the lateral flow strip, allowing the strip's reagents to be nontarget-specific. 91 A typical LFA strip consists of three pads and a detection zone (Figure 2). First, liquid sample is loaded into a sample pad and the analyte moves via capillary flow (without any external forces applied) to the conjugate release pad, where it interacts with specific antibodies conjugated to colored or fluorescent particles, mostly colloidal gold and latex microspheres.…”

Section: Reverse Dot Blots and Lateral Flow Assays-ideal For Low Reso...mentioning

confidence: 99%

“…87 The developed method used a sensitive and broad-spectrum PCR extensive optimization for each new target. 91 On the other hand, NALFIA detects hapten-labeled DNA (mostly exploiting primers tagged with digoxigenin or biotin) using capture and labeled reporter antibodies or streptavidin. 92 This architecture eliminates the need for specific optimization of the lateral flow strip, allowing the strip's reagents to be nontarget-specific.…”

Section: Reverse Dot Blots and Lateral Flow Assays-ideal For Low Reso...mentioning

confidence: 99%

Advanced technologies towards improved HPV diagnostics

Bartosik,

Moranova,

Izadi

et al. 2024

Journal of Medical Virology

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Persistent infection with high‐risk types of human papillomaviruses (HPV) is a major cause of cervical cancer, and an important factor in other malignancies, for example, head and neck cancer. Despite recent progress in screening and vaccination, the incidence and mortality are still relatively high, especially in low‐income countries. The mortality and financial burden associated with the treatment could be decreased if a simple, rapid, and inexpensive technology for HPV testing becomes available, targeting individuals for further monitoring with increased risk of developing cancer. Commercial HPV tests available in the market are often relatively expensive, time‐consuming, and require sophisticated instrumentation, which limits their more widespread utilization. To address these challenges, novel technologies are being implemented also for HPV diagnostics that include for example, isothermal amplification techniques, lateral flow assays, CRISPR‐Cas‐based systems, as well as microfluidics, paperfluidics and lab‐on‐a‐chip devices, ideal for point‐of‐care testing in decentralized settings. In this review, we first evaluate current commercial HPV tests, followed by a description of advanced technologies, explanation of their principles, critical evaluation of their strengths and weaknesses, and suggestions for their possible implementation into medical diagnostics.

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Development of a nucleic acid-based lateral flow device as a reliable diagnostic tool for respiratory viral infections

Cited by 4 publications

References 23 publications

Loop-Mediated Isothermal Amplification and Lateral Flow Immunochromatography Technology for Rapid Diagnosis of Influenza A/B

Loop-Mediated Isothermal Amplification and Lateral Flow Immunochromatography Technology for Rapid Diagnosis of Influenza A/B

An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus

Advanced technologies towards improved HPV diagnostics

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