Development of a novel vector for siRNA delivery based on arginine‐modified polyvinylamine

Abstract

An ideal carrier that delivers small interfering RNA (siRNA) should be designed based on two criteria: high gene transfection efficiency and low cytotoxicity. In this work, arginine was used to modify polyvinylamine (PVAm) to form a novel PVAm derivative (PVAm‐Arg) for siRNA delivery and the derivative was characterized using Fourier transform infrared spectroscopy. PVAm‐Arg/siRNA complexes were formed by the self‐assembly method. The zeta potential and size of the complexes were measured using electrophoretic light scattering and dynamic light scattering, respectively. The siRNA binding capacity of PVAm‐Arg was assessed using gel retardation assay. The results showed that PVAm‐Arg derivatives can bind siRNA effectively and form complexes with sizes of about 72 ± 2 nm. What is more, the ability of PVAm‐Arg to condense siRNA was better than that of PVAm. Cytotoxicity assay with RSC96 cells also showed that PVAm‐Arg had lower cytotoxicity compared with PVAm. In in vitro cellular uptake assay, PVAm‐Arg showed good transfection efficiency. Therefore, we concluded that PVAm‐Arg would be a promising candidate as a gene delivery vector. © 2022 Society of Industrial Chemistry.

Poly(acrylic acid)-b-Poly(vinylamine) Copolymer: Decoration with Silver Nanoparticles, Antibacterial Properties, Quorum Sensing Activity, and Cytotoxicity on Breast Cancer and Fibroblast Cell Lines

“…The absorbance of copolymers and Ag@PAA-b-PVAm was measured with a UV−vis Varian Cary100 spectrophotometer at a wavelength range of 250−800 nm at room temperature. The chemical structures were elucidated using 1 H and 13 C nuclear magnetic resonance (NMR) spectroscopy (Bruker 400 Ultra Shield) at 25 °C. Approximately 50 mg of sample was dissolved in DMSO-d 6 (PAA, PAA-b-PNVF, and PAA-b-PVAm) or CDCl 3 (EPEX).…”

“…This shows that the addition of the PNVF segment is perfectly controlled by the PAA@EPEX macro-CTA, as expected from a typical RAFT polymerization. 1 H and 13 C NMR spectroscopies were used to evaluate the chemical differences of PAA@EPEX and PAA-b-PNVF. The characteristic chemical shifts of PAA were observed at 1.5−1.8 ppm for methylene protons (a) and 2.25 for methyne proton (b), 35 as shown in Figure 3a.…”

“…Overall, the PNVF% values in Table 2 obtained from elemental analyses were supported by the 1 H NMR spectra. 13 C NMR spectra of PAA@EDEX and PAA-b-PNVF block copolymers are given in Figure S3. For PAA@EPEX, methylene and methyne carbon shifts were observed between δ 34−41 ppm, and the carbonyl group resonance at 176 ppm (Figure S3a).…”

“…40 The relative peak intensities of the formamide carbon increased with an increase in PNVF block length, Supporting Information from SEC, elemental analysis, and 1 H NMR spectroscopy. However, the 13 C NMR spectra were not used to calculate the percentages of the copolymer blocks, as the relatively low carbon peak intensities in the spectra may be affected by the background noise.…”

“…NMR spectrum and elemental analysis results for Oethyl-S-(1-phenylethyl) xanthate, SEC results and SEC chromatograms of PAA-EPEX macro-RAFT agent,13 C NMR spectra of the polymers synthesized, TEM image and stability comparison of Ag NPs, disk diffusion assay images, and inverted microscope images (PDF) h i b i t e d .…”