Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in tea

Abstract

The mycotoxin Ochratoxin A (OTA) is responsible for producing many effects on human and animal health. In this work, the evaluation of the presence of OTA in tea beverage samples consisted of extraction and preconcentration through the solidification of a floating organic drop (DLLME-SFO) combined with an additional octadecyl silane clean-up step. The obtained extract was analyzed by UHPLC-MS/MS. Interferences from the matrix were effectively reduced and, consequently, recovery increased from 43.18% ± 4.1%–96.02% ± 2.54%. The validation assays were carried out by external calibration and spiked samples, with satisfactory recoveries. An adequate dynamic calibration range was obtained over a concentration interval between 0.5 and 70 μg mL −1 OTA. Capabilities of detection and quantification were 0.5 and 1.4 μg mL −1 . The obtained Green Certificate was compared with other techniques to establish the greenness profile of the procedure. Quantification of ochratoxin A levels in tea samples was performed.

Rapid Identification and Analysis of Ochratoxin-A in Food and Agricultural Soil Samples Using a Novel Semi-Automated In-Syringe Based Fast Mycotoxin Extraction (FaMEx) Technique Coupled with UHPLC-MS/MS

“…Many research studies developed various extraction steps, such as solid-phase extraction (SPE) cartridges, immuno-affinity SPE columns, floating organic solvents, and dispersive and non-dispersive solvent-based liquid-liquid extraction, to perform in coffee and tea products. However, these methods are time-consuming, laborious, require lots of toxic solvents for extraction, expensive clean-up sorbents, and need highly skilled analysts [ 13 ].…”

Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt

“…Until now, various analytical techniques, such as liquid/gas chromatography coupled to different detectors, have been developed for routine identification and detection of OTA due to their fascinating sensitivity, accuracy and specificity for standard analysis in well-equipped laboratory ( Cina et al, 2021 , Kaya et al, 2019 , Wei et al, 2019 ). However, they are greatly restricted for extensive application by expensive instrument, high cost- and time-consumption, tedious pretreatment, large organic solvent waste, and skilled technicians.…”