Development of a Novel Two Dimensional Surface Plasmon Resonance Sensor Using Multiplied Beam Splitting Optics

Hemmi, Akihide; Mizumura, Ryosuke; Kawanishi, Ryuta; Nakajima, Hirochika; Zeng, Hulie; Uchiyama, Katsumi; Kaneki, Noriaki; Imato, Toshihiko

doi:10.3390/s130100801

Cited by 4 publications

(5 citation statements)

References 16 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This technique gives excellent sensitivity and, as we will show later in this paper, gives dynamic range comparable to white light interferometry ( Ng et al, 2011 ). High throughput multi-point detection has been achieved by a number of different approaches ( Bardin et al, 2009; Hemmi et al, 2013 ). Early examples using amplitude configurations with CCD cameras had sensitivity of around 10 −5 RIU ( Fu et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras

Wang

Smith

Light

et al. 2014

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Phase sensitive Surface Plasmon Resonance (SPR) techniques are a popular means of characterizing biomolecular interactions. However, limitations due to the narrow dynamic range and difficulty in adapting the method for multi-point sensing have restricted its range of applications. This paper presents a compact phase sensitive SPR technology using a custom CMOS camera. The system is exceptionally versatile enabling one to trade dynamic range for sensitivity without altering the optical system. We present results showing sensitivity over the array of better than 10−6 Refractive Index Units (RIU) over a refractive index range of 2×10−2 RIU, with peak sensitivity of 3×10−7 RIU at the center of this range. We also explain how simply altering the settings of polarization components can give sensitivity on the order of 10−8 RIU albeit at the cost of lower dynamic range. The consistent response of the custom CMOS camera in the system also allowed us to demonstrate precise quantitative detection of two Fibrinogen antibody–protein binding sites. Moreover, we use the system to determine reaction kinetics and argue how the multipoint detection gives useful insight into the molecular binding mechanisms.

show abstract

Section: Introductionmentioning

confidence: 99%

Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras

Wang

Smith

Light

et al. 2014

Biosensors and Bioelectronics

View full text Add to dashboard Cite

show abstract

“…For example, Hemmi et al 15 developed this type SPR sensor by utilizing several cubic beam splitters to generate 9 beams for 9 sensing points. However, the incident light intensities of the beams were different due to the fact that beams passed through several beam splitters for the generation of the multi-beams.…”

Section: Introductionmentioning

confidence: 99%

“…The second type of SPR sensor, based on the Kretschmann configuration, is that beam splitters [15][16][17] or a cylindrical lens array 18 can be used to divide a single mono-chromatic incident light beam into multi-beams. For example, Hemmi et al 15 developed this type SPR sensor by utilizing several cubic beam splitters to generate 9 beams for 9 sensing points.…”

Section: Introductionmentioning

confidence: 99%

“…There have been reported two types of optical systems based on the Kretschmann configuration for multi-analyte SPR sensors. [11][12][13][14][15][16][17][18][19][20][21] The first type of SPR sensor is a so-called SPR imaging sensor, where a 2-dimension CCD sensor is used for measuring reflected light from the sensor chip, on which a multi-channel or micro array was fabricated. [11][12][13][14] For example, Piliarik et al 11 reported a high-throughput SPR imaging sensor for nucleic acids, while identifying a specific bacteria pathogenous.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor

et al. 2016

Self Cite

View full text Add to dashboard Cite

A portable-type surface plasmon resonance (SPR) sensor, composed from a new optical system for multi-sensing, has been developed to apply to environment analysis, clinical diagnosis etc., where many samples are desired to be analyzed at high throughput. The optical system of the sensor consists of a light-emitting diode, a pair of cylindrical lenses, a pair of collimator lenses, a correction lens, a prism, a polarizer and a linear CCD sensor with 2048 pixels. Reflected light from a sensor chip of the width of 6 mm at a certain incident angle was detected by ca. 618 pixels of the linear CCD sensor as an SPR sensor signal. An SPR sensor signal at a specified incident angle is controllable for optimization by adjusting the position of the CCD sensor. A sensor chip having a 30-stripe linear pattern (100 μm width/stripe) was prepared. The spatial resolution as well as the performance of the sensor were evaluated by using sucrose solutions. As a result, the acquisition of SPR sensor signals from 30 sensing points was successfully achieved with a spatial resolution of 100 μm (distance between 2 sensing points). A lower detection limit of ca. 3.2 -5.5 × 10 -5 RIU with a standard deviation of ±4.5% was obtained by averaging the signals from 6 -7 pixels of the CCD sensor per one sensing stripe.

show abstract

“…Al utilizar la técnica de SPR es posible medir el cambio en el índice de refracción del entorno dieléctrico sobre la superficie de un metal [8].…”

Section: Fabricación De Un Biosensor De Celda Microfluídica Para La Eunclassified

Fabricación de un Biosensor de Celda Microfluídica para la Estandarización de Muestras de ADN Metilado en Individuos con Obesidad y Diabetes

Arenas-Márquez¹,

Hernández-Como²,

Hernández-López³

et al.

View full text Add to dashboard Cite

Resumen-La integración de técnicas de microfluídica y plataformas de detección de resonancia de plasmones superficiales (SPR) tiene la ventaja de contar con una gran sensibilidad en tiempo real en la detección de bajas concentraciones de ADN. El presente trabajo tiene como objetivo la fabricación de un biosensor que al combinar una celda microfluídica sobre un sensor de SPR permita la estandarización de muestras para determinar la metilación de los genes FTO y ABCC8 (SUR 1) empleando este. Las muestras fueron amplificadas a partir de sangre periférica de individuos con obesidad y diabetes. El biosensor de celda microfluídica fue fabricado mediante técnicas de microfabricación utilizando Polidimetilsiloxano (PDMS) y un vidrio con películas delgadas de Cr/Au. El cual funciona a partir de una muestra de al menos 20 μl y permite detectar ADN en una concentración del orden de nanogramos por microlitro.Palabras clave-Celda microfluídica, Diabetes, Metilación, Obesidad.I. INTRODUCCIÓN La epigenética ayuda a explicar los mecanismos no dependientes de la secuencia genética por los que los nutrientes y otros factores ambientales contribuyen a regular la expresión de los genes. Su naturaleza reversible abre la puerta no sólo a comprender, sino también a tratar enfermedades de origen poligénico y multifactorial, como son la obesidad y la diabetes. Producto de la relativa facilidad de análisis de genes específicos, la metilación del ADN es la más ampliamente estudiada en investigaciones que relacionan los efectos sobre la salud de los cambios epigenéticos, incluyendo la obesidad. La metilación del ADN es una modificación química de la esctructura inducida por grupos de enzimas conocidas como ADN metil transferasas que unen covalentemente un grupo metilo (-CH3) al carbono 5 de la citosina. Se le ha asociado a procesos metabólicos clave relacionados con la obesidad y diabetes [1].Esto puede ser una poderosa herramienta para entender la etiología y tratamiento de muchas enfermedades y a medida que se conozcan los cambios específicos que caracterizan a cada condición patológica podremos entender mejor los mecanismos y determinar conductas más eficaces para asegurar la salud del humano [2].Durante las últimas décadas, el desarrollo de la biotecnología ha realizado diferentes estudios para comenzar a atender las necesidades de desarrollar dispositivos para el diagnóstico molecular, que sean de alto rendimiento, miniaturizados y capaces de ser producidos de forma masiva, para cumplir con tal fin, se ha explotado el área de biosensores [3].La fabricación y aplicación de biosensores presenta mecanismos de detección de un amplio rango de biomoléculas de interés en la industria y en el diagnóstico clínico, a través de diversos fenómenos físicos como la variación de masa, de esfuerzo de resistencia y del índice de refracción. Por lo que la detección de la metilación es un punto esencial, existen diferentes métodos de detección como técnicas de biología molecular, estrategias basadas en la electroquímica, en transistores de efecto de cam...

show abstract

Development of a Novel Two Dimensional Surface Plasmon Resonance Sensor Using Multiplied Beam Splitting Optics

Cited by 4 publications

References 16 publications

Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras

Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras

Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor

Fabricación de un Biosensor de Celda Microfluídica para la Estandarización de Muestras de ADN Metilado en Individuos con Obesidad y Diabetes

Contact Info

Product

Resources

About