The influence of volatilization from mainstream tobacco smoke particles in annular denuders on the gas-particle distribution was investigated by using multiple annular denuders coupled with a filter pack method (multiple-AD-FP method) under low flow volume conditions (1.05 and 1.65 L/min). Smoking experiments and model experiments using poly styrene latex (PSL) particles and generated gases were conducted to measure the particle loss ratio and gas collection efficiency in the denuders. In this research, we focused on the influence of XAD-4 slurry concentrations and particle volatility including tobacco smoke from a cigarette and vapor products on the volatilization from particles in the denuders, and have proposed a measurement method for considering volatilization. Our experimental results showed that volatilization depends on the particle volatility, targeted components, denuder length, particle concentration, and adsorbent amount, thereby suggesting the importance of selecting the proper measurement conditions and taking volatilization into account, so as to evaluate the appropriate gas-particle distribution. The gas-phase nicotine ratio obtained from calculating the volatilization rate in the multiple-AD-FP method agreed with previous research. This means that the calculation method was appropriate for the evaluation of gas-particle distribution. Furthermore, the gas-particle ratio determined through the multiple-AD-FP method was compared to that determined through a filter-impinger method that uses a glass fiber filter. The results revealed that most of the gases in the filter-impinger method were not collected in the impinger due to the condensation of gases on the surface of the filter fibers and collected particles.