Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein

Chumbe, Ana; Izquierdo-Lara, Ray; Calderón, Katherine; Fernández-Díaz, Manolo; Vakharia, Vikram N.

doi:10.1186/s12985-017-0900-8

Cited by 23 publications

(28 citation statements)

References 44 publications

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“…However, it is highly laborious and very slow, yielding results only after nearly one week. Interestingly, Chumbe et al [ 75 ] reported the development of an improved VNT using a recombinant NDV engineered to constitutively express GFP. The newly developed assay has been shown to give conclusive results within 24 hours without the need for any additional staining procedure.…”

Section: Diagnosismentioning

confidence: 99%

Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives

Bello

Yusoff

Ideris

et al. 2018

BioMed Research International

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Newcastle disease (ND) is one of the most devastating diseases that considerably cripple the global poultry industry. Because of its enormous socioeconomic importance and potential to rapidly spread to naïve birds in the vicinity, ND is included among the list of avian diseases that must be notified to the OIE immediately upon recognition. Currently, virus isolation followed by its serological or molecular identification is regarded as the gold standard method of ND diagnosis. However, this method is generally slow and requires specialised laboratory with biosafety containment facilities, making it of little relevance under epidemic situations where rapid diagnosis is seriously needed. Thus, molecular based diagnostics have evolved to overcome some of these difficulties, but the extensive genetic diversity of the virus ensures that isolates with mutations at the primer/probe binding sites escape detection using these assays. This diagnostic dilemma leads to the emergence of cutting-edge technologies such as next-generation sequencing (NGS) which have so far proven to be promising in terms of rapid, sensitive, and accurate recognition of virulent Newcastle disease virus (NDV) isolates even in mixed infections. As regards disease control strategies, conventional ND vaccines have stood the test of time by demonstrating track record of protective efficacy in the last 60 years. However, these vaccines are unable to block the replication and shedding of most of the currently circulating phylogenetically divergent virulent NDV isolates. Hence, rationally designed vaccines targeting the prevailing genotypes, the so-called genotype-matched vaccines, are highly needed to overcome these vaccination related challenges. Among the recently evolving technologies for the development of genotype-matched vaccines, reverse genetics-based live attenuated vaccines obviously appeared to be the most promising candidates. In this review, a comprehensive description of the current and emerging trends in the detection, identification, and control of ND in poultry are provided. The strengths and weaknesses of each of those techniques are also emphasised.

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Section: Diagnosismentioning

confidence: 99%

Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives

Bello

Yusoff

Ideris

et al. 2018

BioMed Research International

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show abstract

“…The clinical symptoms of ND are similar to those of some other diseases including low pathogenic avian influenza, infectious laryngotracheitis, and infectious bronchitis, all showing the respiratory sign of dyspnea, so they may be confused with each other in the clinical diagnosis ( Chumbe et al., 2017 , Giovanni et al., 2017 ). A specific, sensitive, and convenient diagnostic method can provide strong support for the prevention and treatment of this disease.…”

Section: Introductionmentioning

confidence: 99%

Establishment of reverse transcription recombinase–aided amplification-lateral-flow dipstick and real-time fluorescence–based reverse transcription recombinase–aided amplification methods for detection of the Newcastle disease virus in chickens

Wang

Bai

et al. 2020

Poultry Science

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Newcastle disease is an acute and highly contagious disease of poultry caused by Newcastle disease virus infection, which does great harm to the poultry industry all over the world. To diagnose the disease simply and quickly, 2 detection methods were established based on reverse transcription recombinase–aided amplification ( RT-RAA ) technology. One is reverse transcription recombinase–aided amplification-lateral flow dipstick ( RT-RAA-LFD ) that is to combine RT-RAA with lateral flow dipstick; the other is real-time fluorescence-based reverse transcription recombinase–aided amplification ( RF-RT-RAA ) that is the combination of RT-RAA and exo probe. In this study, the reaction conditions such as reaction temperature and reaction time of the 2 methods were optimized, and their specificity and sensitivity were tested. The results showed that the RT-RAA-LFD method could be used to complete reaction within 23 min, and its lowest detectable limit was 10 2 copies/μL, 10 times higher than that of the conventional PCR method (10 3 copies/μL); the RF-RT-RAA method could be used to complete reaction within 26 min, and its lowest detectable limit was 10 copies/μL, 100 times higher than that of conventional PCR method (10 3 copies/μL), and it was as sensitive as real-time fluorescence–based quantitative PCR (10 copies/μL). The 2 methods had no cross reaction to the nucleic acid of other avian pathogens and showed good specificity. A total of 86 clinical samples suspected of the Newcastle disease virus were tested by conventional PCR, real-time fluorescence–based quantitative PCR, RT-RAA-LFD, and RF-RT-RAA. Based on the commonly used conventional PCR method, the other 3 detection methods had a coincidence rate of higher than 93%. In summary, RT-RAA-LFD and RF-RT-RAA had high specificity, sensitivity, and efficiency, which were suitable for clinical and laboratory diagnosis, respectively, and provided technical support for the prevention and control of Newcastle disease.

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“…The serum samples from different vaccinated groups, taken at day 3 wpi, were subjected to HI assay using 1% chicken red blood cells in accordance with the standard method ( Alexander, 2000 ). The antibody titer was measured by VN assay with modifications ( Chumbe et al., 2017 ). Briefly, monolayer cultures of CEF cells were seeded at 80–90% confluence in 96-well plates.…”

Section: Methodsmentioning

confidence: 99%

Generation and evaluation of a vaccine candidate of attenuated and heat-resistant genotype VIII Newcastle disease virus

et al. 2020

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Newcastle disease, which is a highly contagious and fatal disease caused by the Newcastle disease virus ( NDV ), has harmed the poultry industry for decades. The administration of effective vaccines can control most outbreaks and epidemics of Newcastle disease in the world. However, vaccination failures of live attenuated vaccines becasue of storage and transportation problems have been reported. Hence, thermostable live vaccine strains, such as V4 and I-2 strains, are being used and welcomed in tropical regions such as Africa and Southeast Asia. In this study, a thermostable, attenuated vaccine candidate strain NDV/rHR09 was generated using the genotype VIII heat-resistant virulent NDV strain HR09 by the reverse genetics system. The results of the determination of the mean death time and intracerebral pathogenicity index indicated that NDV/rHR09 is lentogenic even after 15 serial passages in embryonated chicken eggs. The thermostability assessment showed that the NDV/rHR09 strain exhibited hemagglutination activity and infectivity when exposed to 56°C for 60 min. Compared with the commercially available La Sota and V4 vaccines, the NDV/rHR09 induced higher antibody titers in specific pathogen-free chickens. In addition, NDV/rHR09 conferred complete protection against virulent genotype VII NDV challenge and virus shedding from vaccinated chickens. These results suggest that NDV/rHR09 is a promising thermostable vaccine candidate strain.

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Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein

Cited by 23 publications

References 44 publications

Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives

Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives

Establishment of reverse transcription recombinase–aided amplification-lateral-flow dipstick and real-time fluorescence–based reverse transcription recombinase–aided amplification methods for detection of the Newcastle disease virus in chickens

Generation and evaluation of a vaccine candidate of attenuated and heat-resistant genotype VIII Newcastle disease virus

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