Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products

Hu, Lijun; M., Li; Zheng, Shangen; He, Xiaohua; Hammack, Thomas S.; Brown, Eric W.; Zhang, Guodong

doi:10.1016/j.foodcont.2018.01.006

Cited by 31 publications

(7 citation statements)

References 23 publications

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“…A few LAMP assays have been developed that target specific Salmonella serovars or serogroups ( Table 2 ). For instance, sdfI (Yang et al , 2010 ) and prot6E (Hu et al , 2018 ) were used to design two separate LAMP assays for Salmonella enterica serovar Enteritidis, while typh was used to specifically detect Salmonella Typhimurium (Kumar et al , 2014 ). The sefA gene has been explored to design a LAMP assay for both Salmonella Enteritidis and Salmonella Gallinarum (Gong et al , 2016 ).…”

Section: Salmonella Lamp Assay Developmentmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Yang

Domesle

2018

Foodborne Pathogens and Disease

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Loop-mediated isothermal amplification (LAMP) has become a powerful alternative to polymerase chain reaction (PCR) for pathogen detection in clinical specimens and food matrices. Nontyphoidal Salmonella is a zoonotic pathogen of significant food and feed safety concern worldwide. The first study employing LAMP for the rapid detection of Salmonella was reported in 2005, 5 years after the invention of the LAMP technology in Japan. This review provides an overview of international efforts in the past decade on the development and application of Salmonella LAMP assays in a wide array of food and feed matrices. Recent progress in assay design, platform development, commercial application, and method validation is reviewed. Future perspectives toward more practical and wider applications of Salmonella LAMP assays in food and feed testing are discussed.

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Section: Salmonella Lamp Assay Developmentmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Yang

Domesle

2018

Foodborne Pathogens and Disease

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“…The reaction time of multiplex real‐time RPA was 20 min, and the temperature is 35°C in our RPA assay. LAMP assays can also be used to detect Salmonella from foods, environmental samples at 60°C ‐65°C within 30–60 min of reaction time (Gong et al, 2016; Hu et al, 2018; Mashooq, Kumar, Niranjan, Agarwal, & Rathore, 2016; Techathuvanan & D'Souza, 2012; Techathuvanan, Draughon, & D'Souza, 2010; Yang, Domesle, Wang, & Ge, 2016; Zhang, Pan, et al, 2012; Zhang, Shan, et al, 2012). Zhang, Shan, et al (2012) developed a method for detection of Salmonella in food with a LOD for pure culture of 16 CFU/reaction and a sensitivity in food samples of 93.6%.…”

Section: Discussionmentioning

confidence: 99%

Detection of Salmonella enteritidis and Salmonella typhimurium in foods using a rapid, multiplex real‐time recombinase polymerase amplification assay

Ren

Man

et al. 2020

Journal of Food Safety

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Salmonella has been recognized as a major foodborne pathogen for humans and animals. In this study, a multiplex real‐time recombinase polymerase amplification (RPA) was developed for simultaneous detection of Salmonella enterica serovars, Salmonella enteritidis and Salmonella typhimurium, from chicken, eggs, lettuce, and papaya. The reaction was performed for 20 min at 35°C, and the detection limit of the assay was 102 CFU/ml for pure culture. In food application, the limit of detection (LOD) of S. enteritidis and S. typhimurium using multiplex real‐time RPA without enrichment procedure was 102 CFU/25 g, respectively. After enrichment, the LOD of S. enteritidis and S. typhimurium was 10 CFU/25 g. Moreover, the result for Salmonella spp. was not significantly different from those obtained using a culture‐based method. Additionally, the assay has a lower cross‐reactivity with other pathogenic microorganisms and a good stability performance. Thus, the developed multiplex RPA assay could be used as a rapid tool for the detection of S. enteritidis and S. typhimurium in food.

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“…The amplified products from the LAMP reaction are not single-size amplicons but rather exhibit a range of different product sizes ( Supplementary Figure S2 , Part 5b). Thus, a positive LAMP reaction appears as a smear or a ladder of amplified products on an agarose gel, rather than a single band as seen with PCR ( Hill et al, 2008 ; Tang et al, 2012 ; Wang et al, 2015 ; Lin et al, 2017 ; Hu et al, 2018 ). For detection of Enterococcus spp.…”

Section: Methodsmentioning

confidence: 99%

“…spp. ), Staphylococcus aureus (S. aureus) , methicillin-resistant Staphylococcus aureus (MRSA), and Enterococcus ( Hill et al, 2008 ; Tang et al, 2012 ; Wang et al, 2015 ; Lin et al, 2017 ; Hu et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) as a Rapid, Affordable and Effective Tool to Involve Students in Undergraduate Research

et al. 2020

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Undergraduate research (UR) is a high-impact practice (HIP) to engage undergraduate student in science, technology, engineering and mathematics (STEM), especially from underrepresented groups. UR experiences (UREs) can be integrated into the classroom, making authentic research experiences inclusive and available to all students. However, developing UR pedagogy can be challenging for faculty in resource-limited labs, such as community colleges and small liberal arts colleges. Often molecular biology research methods are expensive, time-consuming and need equipment not readily available or affordable in small schools. Polymerase chain reaction (PCR) is one of the most commonly used techniques in research labs and many UREs. We have investigated loop-mediated isothermal amplification (LAMP) as an inexpensive, accessible alternative to PCR for DNA amplification enabling the identification of microorganisms in the context of UREs. LAMP does not require expensive instrumentation or reagents and uses equipment commonly found in teaching labs. By performing the technique, students learn several key scientific skills that will be useful in their undergraduate or graduate STEM careers. We designed guided independent research experiences for several undergraduates that included the use of LAMP. Students successfully applied the technique to culture samples of common environmental bacteria, including Escherichia coli, Salmonella spp., Staphylococcus aureus, and Enterococcus, and were in addition, able to detect both Salmonella and Enterococcus in directly sampled environmental waters. To highlight the accessibility and affordability of this URE, a simple boiling method was used for DNA preparation from environmental samples. Student response data show positive attitudes toward UR when LAMP is utilized as a research tool to tackle relevant biological questions. The feasibility of using simplified LAMP in UREs points to a potential, more expanded application to public engagement with science and broader and more inclusive interactions with the research community.

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Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products

Cited by 31 publications

References 23 publications

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Detection of Salmonella enteritidis and Salmonella typhimurium in foods using a rapid, multiplex real‐time recombinase polymerase amplification assay

Loop-Mediated Isothermal Amplification (LAMP) as a Rapid, Affordable and Effective Tool to Involve Students in Undergraduate Research

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