Development of a novel immunoassay for the simple and fast quantitation of neutrophil gelatinase-associated lipocalin using europium(III) chelate microparticles and magnetic beads

Wang, Hao; Zhai, Xiang-Ming; Liu, Tiancai; Liang, Junyu; Bian, Lun; Chen, Zhenhua; Dong, Zhi-Ning; Li, Zhixiong; Wu, Yixuan

doi:10.1016/j.jim.2019.04.004

Cited by 9 publications

(2 citation statements)

References 24 publications

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“…Fifthly, we used the methods of electrochemical luminescence and chemiluminescent microparticle immunoassay for testing tumor markers, some new methods with high sensitivity and specificity may be more helpful for early detection of tumor markers (39,40). Last but not least, though weighting method were used to balance all measurable baseline covariates, some unrecorded baseline covariates such as TMB could be potential confounders.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Serum Tumor Markers Can Serve as a Prognostic Biomarker for Chinese Advanced Non-small Cell Lung Cancer Patients Treated With Immune Checkpoint Inhibitors

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Dynamics of Serum Tumor Markers Can Serve as a Prognostic Biomarker for Chinese Advanced Non-small Cell Lung Cancer Patients Treated With Immune Checkpoint Inhibitors

et al. 2020

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“…According to the literature, the expression of IL‐18 is elevated in the serum of patients with hepatitis B 12 and liver cancer 13 compared to healthy controls. Therefore, we aimed to establish a highly sensitive time‐resolved fluorescence immunoassay (TRFIA) 14 and evaluate its application for detecting IL‐18 in the serum of liver cancer, hepatitis B, hepatitis C, autoimmune hepatitis, and fatty liver disease patients.…”

Section: Introductionmentioning

confidence: 99%

Establishment of interleukin‐18 time‐resolved fluorescence immunoassay and its preliminary application in liver disease

Zhang

Zhou

Dai

et al. 2021

Clinical Laboratory Analysis

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Background To establish a time‐resolved fluorescence immunoassay of interleukin (IL)‐18 (IL‐18‐TRFIA) and detect its concentration in different liver disease serum samples. Methods The IL‐18 coating antibody and the Eu3+‐labeled detection antibody were used for the IL‐18‐TRFIA to detect serum IL‐18 concentration in patients with liver cancer, hepatitis B, hepatitis C, autoimmune hepatitis, fatty liver disease, and healthy controls. The double‐antibody sandwich method was used and methodological evaluation was performed. Results The average intra‐ and inter‐assay coefficient of variation for IL‐18‐TRFIA was 4.80% and 5.90%, respectively. The average recovery rate was 106.19 ± 3.44%. The sensitivity (10.96 pg/mL) was higher than that obtained using the ELISA method (62.5 pg/mL). The detection range was 10.96–1000 pg/mL. IL‐6 and galectin‐3 did not cross‐react with IL‐18‐TRFIA. The serum concentration of IL‐18 was (776.99; 653.48–952.39 pg/mL) in hepatitis C, (911; 775.55–1130.03 pg/mL) in fatty liver, (1048.88; 730.04–1185.10 pg/mL) in liver cancer, and (949.12; 723.70–1160.28 pg/mL) in hepatitis B. Moreover, IL‐18 serum levels were significantly higher in patients than the healthy controls (483.09; 402.52–599.70/mL) (p < 0.0001). Autoimmune hepatitis with a serum IL‐18 concentration of 571.62; 502.47–730.31 pg/mL was not significantly different from the healthy controls (p > 0.05). Conclusion We established a highly sensitive IL‐18‐TRFIA method that successfully detected serum IL‐18 concentrations in different liver diseases. Furthermore, IL‐18 serum concentration was higher in patients with liver cancer, hepatitis C, hepatitis B, and fatty liver disease compared to healthy controls.

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Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf

Awaludin

Abdullah

Salam

et al. 2020

Analytical Biochemistry

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The identification of rice bacterial leaf blight disease requires a simple, rapid, highly sensitive, and quantitative approach that can be applied as an early detection monitoring tool in rice health. This paper highlights the development of a turn-off fluorescence-based immunoassay for the early detection of Xanthomonas oryzae pv. oryzae (Xoo), a gram-negative bacterium that causes rice bacterial leaf blight disease. Antibodies against Xoo bacterial cells were produced as specific biorecognition molecules and the conjugation of these antibodies with graphene quantum dots and gold nanoparticles was performed and characterized, respectively. The combination of both these bio-probes as a fluorescent donor and metal quencher led to changes in the fluorescence signal. The immunoreaction between AntiXoo-GQDs, Xoo cells, and AntiXoo-AuNPs in the immunoaggregation complex led to the energy transfer in the turn-off fluorescence-based quenching system. The change in fluorescence intensity was proportional to the logarithm of Xoo cells in the range of 100-105 CFU mL−1. The limit of detection was achieved at 22 CFU mL−1 and the specificity test against other plant disease pathogens showed high specificity towards Xoo. The detection of Xoo in real plant samples was also performed in this study and demonstrated satisfactory results.

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Development of a novel immunoassay for the simple and fast quantitation of neutrophil gelatinase-associated lipocalin using europium(III) chelate microparticles and magnetic beads

Cited by 9 publications

References 24 publications

Dynamics of Serum Tumor Markers Can Serve as a Prognostic Biomarker for Chinese Advanced Non-small Cell Lung Cancer Patients Treated With Immune Checkpoint Inhibitors

Dynamics of Serum Tumor Markers Can Serve as a Prognostic Biomarker for Chinese Advanced Non-small Cell Lung Cancer Patients Treated With Immune Checkpoint Inhibitors

Establishment of interleukin‐18 time‐resolved fluorescence immunoassay and its preliminary application in liver disease

Fluorescence-based immunoassay for the detection of Xanthomonas oryzae pv. oryzae in rice leaf

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