Development of a novel, FTIR (Fourier transform infrared spectroscopy) based, yeast bioassay for toxicity testing and stress response study

Corte, Laura Della; Rellini, Paolo; Roscini, Luca; Fatichenti, F.; Cardinali, Gianluigi

doi:10.1016/j.aca.2009.11.035

Cited by 78 publications

(77 citation statements)

References 41 publications

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“…The authors also observed increased Raman intensity for lipids and some proteins but not for nucleic acids as a function of nanoparticle concentration indicating that nanoparticleinduced injury is likely caused by a different mechanism than antibiotic treatment in which protein and nucleic acid synthesis are inhibited. In other studies, FT-IR was used to study heat-induced lethal and sublethal injuries in Lactococcus lactis (Kilimann et al 2006), osmotic and thermally induced injuries of E. coli (Mille et al 2002), UV radiationinduced injury of Staphylococcus aureus (Krishnamurthy et al 2010), lethal injury in E. coli caused by dehydration (Beney et al 2004), radical-induced damage of Micrococcus luteus (Lorin-Latxague and Melin 2005), acid tolerance response of Streptococcus macedonicus (Papadimitriou et al 2008), and toxicity response of yeast (Corte et al 2010). Recently, the membrane phase behavior of E. coli during desiccation and rehydration was also studied by FT-IR (Scherber et al 2009).…”

Section: Studies Of Bacterial Injury and Inactivation By Vibrational mentioning

confidence: 99%

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Al-Qadiri

Lin

et al. 2011

Food Bioprocess Technol

202

120

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Infrared spectroscopy and Raman spectroscopy provide complementary technologies for rapid and precise detection of microorganisms and are emerging methods in food analysis. It is possible to use either of these techniques to differentiate and quantify microorganisms in relatively simple matrices such as liquid media and simple solutions with determinations taking less than an hour. Vibrational spectroscopy, unlike other techniques used in microbiology, is a relatively simple method for studying structural changes occurring within a microbial cell following environmental stress and applications of food processing treatments. Vibrational spectroscopy provides a wide range of biochemical properties about bacteria in a single spectrum, most importantly characteristics of the cell membrane. These techniques are especially useful for studying properties of bacterial biofilms on contact surfaces, the presence and viability of bacterial vegetative cells and spores, the type and degree of bacterial injury, and assessment of antibiotic susceptibility. Future trends in food analysis will involve combining vibrational spectroscopy with microscopy, mass spectroscopy, or DNA-based methods to comprehensively study bacterial stress. Further advances in selectivity, sensitivity, and improved chemometric methods, along with reduction in the cost of instrumentation, may lead to the development of fieldready and real-time analytical systems.

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Section: Studies Of Bacterial Injury and Inactivation By Vibrational mentioning

confidence: 99%

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Al-Qadiri

Lin

et al. 2011

Food Bioprocess Technol

202

120

View full text Add to dashboard Cite

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“…A common approach to improve detection of metabolites that differ in characteristics of ionization or separation is to combine results from multiple analytic platforms. Innovative approaches include biosensor arrays, microfluidics, and alternate spectral methods, such as Fourier-transform infrared spectroscopy (13; 54; 107). An important advance from the commercial sector (Metabolon, Research Triangle Park, NC) is the introduction of a hybrid approach for metabolomics, consisting of targeted analysis of 300 to 500 known chemicals and untargeted analysis of an additional 3000 unidentified m/z .…”

Section: Profiling Metabolites For Nutritional Metabolomicsmentioning

confidence: 99%

Nutritional Metabolomics: Progress in Addressing Complexity in Diet and Health

2012

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Nutritional metabolomics is rapidly maturing to use small molecule chemical profiling to support integration of diet and nutrition in complex biosystems research. These developments are critical to facilitate transition of nutritional sciences from population-based to individual-based criteria for nutritional research, assessment and management. This review addresses progress in making these approaches manageable for nutrition research. Important concept developments concerning the exposome, predictive health and complex pathobiology, serve to emphasize the central role of diet and nutrition in integrated biosystems models of health and disease. Improved analytic tools and databases for targeted and non-targeted metabolic profiling, along with bioinformatics, pathway mapping and computational modeling, are now used for nutrition research on diet, metabolism, microbiome and health associations. These new developments enable metabolome-wide association studies (MWAS) and provide a foundation for nutritional metabolomics, along with genomics, epigenomics and health phenotyping, to support integrated models required for personalized diet and nutrition forecasting.

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“…Similarly, the methods of extraction, quenching, and sample storage conditions can affect and potentially modify metabolite structure, thereby confounding already complex data sets and introducing greater sample-to-sample variability. [22][23][24][25][26] NMR exploits the behavior of molecules when placed in a magnetic field, allowing the identification of different nuclei based on their resonant frequency. 20 Although MS is more sensitive than NMR, NMR spectroscopy has the advantages of being relatively robust across many samples, relatively fast in addition to being a non-destructive process.…”

Section: Introductionmentioning

confidence: 99%

Applications of NMR spectroscopy based metabolomics: a review

Yoon¹,

Lee²,

Kim³

et al. 2013

Journal of the Korean Magnetic Resonance Society

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Metabolomics is the study which detects the changes of metabolites level. Metabolomics is a terminal view of the biological system. The end products of the metabolism, metabolites, reflect the responses to external environment. Therefore metabolomics gives the additional information about understanding the metabolic pathways. These metabolites can be used as biomarkers that indicate the disease or external stresses such as exposure to toxicant. Many kinds of biological samples are used in metabolomics, for example, cell, tissue, and bio fluids. NMR spectroscopy is one of the tools of metabolomics. NMR data are analyzed by multivariate statistical analysis and target profiling technique. Recently, NMR-based metabolomics is a growing field in various studies such as disease diagnosis, forensic science, and toxicity assessment.

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Development of a novel, FTIR (Fourier transform infrared spectroscopy) based, yeast bioassay for toxicity testing and stress response study

Cited by 78 publications

References 41 publications

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Nutritional Metabolomics: Progress in Addressing Complexity in Diet and Health

Applications of NMR spectroscopy based metabolomics: a review

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