Development of a novel enzymatic biosensor based on an ion-selective field effect transistor for the detection of explosives

Комарова, Н. В.; Andrianova, Mariia; Gubanova, O. V.; Кузнецов, Е. В.; Kuznetsov, Alexander

doi:10.1016/j.snb.2015.07.015

Cited by 23 publications

(7 citation statements)

References 28 publications

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“…A key element in this strategy is the remote, surreptitious detection of the presence of residual explosive components at or near the sites, providing confirmation of illicit activity and allowing authorities to coordinate efforts to identify and detain persons associated with IED manufacture. As current detection methods for readily available nitrogen-based explosives continue to evolve in terms of sensitivity and selectivity, [1][2][3][4][5] enemy combatants are increasingly turning to alternate explosives based on chlorate or peroxides, for which fewer means of reliable detection in the field are available.…”

Section: Introductionmentioning

confidence: 99%

Statistical evaluation of an electrochemical probe for the detection of chlorate

Trammell

Shriver‐Lake

Dressick

2017

Sensors and Actuators B: Chemical

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We describe the use and characterization of a multilayer film, prepared via layer-by-layer deposition of cationic para-rosaniline acetate dye (i.e., PR) and the vanadium-containing Keggintype polyoxometalate anion, [PMo 11 VO 40 ] 5 (i.e., PVMo 11 ), on indium tin oxide (ITO) as an electrode for determination of chlorate. A linear cyclic voltammetry (CV) current response was observed for the 0 M  [ClO 3  ]  1000 M range with a detection limit of ~220 M ClO 3  (S/N > 3) in a pH = 2.5 solution of 100 mM sodium acetate. Electrode response was insensitive to interference by oxygen and nitrogen-based explosives like TNT, a prerequisite for use in the field. A Taguchi L16 array was used to investigate the performance of the electrode as functions of number of PVMo 11 /PR bilayers (L; 3-6 bilayers), solution pH (H; pH ~1.32 -2.85), solution [ClO 3  ] (C; 250 -1000 M), CV scan rate (S; 50-200 mVs 1 ), and film age (A; 1-8 weeks). Maximum current response was obtained for a 1 week old 5 bilayer film immersed in a pH 2.85 0.10 M NaCl (aq) solution containing 1000 M ClO 3  . However, current response fell as films aged, requiring aging of films for approximately 8 weeks prior to use to obtain reproducible analyses. A two-level full factorial design using films aged 8 weeks identified the variables S, L, H, and C and variable interactions LS and HS as statistically significant contributors to the film current response and provided a model describing film performance.

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Section: Introductionmentioning

confidence: 99%

Statistical evaluation of an electrochemical probe for the detection of chlorate

Trammell

Shriver‐Lake

Dressick

2017

Sensors and Actuators B: Chemical

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“…In the case of TNT, the dynamic range of the analytical response ranged between 10 −7 and 10 −5 mol•L −1 . In addition, this biosensor was combined with a microfluidic system for analyte delivery and applied to the determination of explosives in water samples [127].…”

Section: Electrochemical Biosensors For Chemical and Biological Weaponsmentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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“…Since being invented by Lieber’s group in 2001, silicon nanowire field-effect transistor (SiNW-FET) sensors have received particular interests because of their high sensitivity for label-free and real-time detection. , In fact, ultrasensitive SiNW-FET sensors have been successfully employed for the real-time and label-free detection of proteins, − nucleic acids, − viruses, , and different targeted substances. ,− Moreover, in comparison with other nanostructure-based sensors, the SiNW-FET sensors have more commercial potential due to their feasibility for mass production in semiconductor industry, especially by the so-called top–down process . However, applying FET nanosensors in clinical trials to detect biomolecules is severely hindered by the ionic screening effect, also known as Debye screening, caused by the high ionic strength of physiological environment (>100 mM) .…”

Section: Introductionmentioning

confidence: 99%

Signal Enhancement of Silicon Nanowire Field-Effect Transistor Immunosensors by RNA Aptamer

Yang

et al. 2019

ACS Omega

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Silicon nanowire field-effect transistors (SiNW-FETs) have been demonstrated as a highly sensitive platform for label-free detection of a variety of biological and chemical entities. However, detecting signal from immunoassays by nano-FETs is severely hindered by the distribution of different charged groups of targeted entities, their binding orientation, and distances to the surface of the FET. Aptamers have been widely applied as a recognition element for plentiful biosensors because of small molecular sizes and moderate to high specific binding affinity with different types of molecules. In this study, we propose an effective approach to enhance the electrical responses of both direct (6×-histidine) and sandwich (amyloid β 1–42) immunoassays in SiNW-FETs with R18, a highly negative charged RNA aptamer against rabbit immunoglobulin G (IgG). Empirical results presented that the immunosensors targeted with R18 expressed a significantly stabilized and amplified signal compared to the ones without this aptamer. The research outcome provides applicability of the highly negative charged aptamer as a bioamplifier for immunoassays by FETs.

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Development of a novel enzymatic biosensor based on an ion-selective field effect transistor for the detection of explosives

Cited by 23 publications

References 28 publications

Statistical evaluation of an electrochemical probe for the detection of chlorate

Statistical evaluation of an electrochemical probe for the detection of chlorate

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

Signal Enhancement of Silicon Nanowire Field-Effect Transistor Immunosensors by RNA Aptamer

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