Development of a Novel Electromagnetic Rewarming Technology for the Cryopreservation of Stem Cells with Large Volume

Ren, Shen; Shu, Zhiquan; Pan, Jiaji; Ji, Peng; Wang, Junlan; Zhao, Chunhua; Gao, Dayong

doi:10.5772/intechopen.94556

Cited by 5 publications

(7 citation statements)

References 47 publications

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“…In previous studies [33,35,47], a mathematical model of the effect of acoustic fields on reproductive cells during cryopreservation was constructed. This applies to all stages of cryopreservation (mixing with a cryoprotectant, diffusion, and temperature jump).…”

Section: Discussionmentioning

confidence: 99%

“…Numerical implementation of the constructed model using the finite element method allows us to study the temperature change inside the vessel. From experimental works [33,35], it is known that, depending on the freezing protocol, there may be a temperature jump, which affects the quality of the frozen material. Using the constructed mathematical model and its numerical implementation, modes and areas of occurrence of temperature jumps can be identified; therefore, it is possible to optimize both the cryopreservation protocol itself and the geometry of the vessel.…”

Section: Mathematical Model For Crystallizationmentioning

confidence: 99%

“…Another direction for optimizing cryopreservation protocols is additional external influence on the suspension, for example, by external acoustic fields [33][34][35][36]. Cryopreservation with simultaneous exposure to germ cells by acoustic-mechanical fields contributes to a more uniform distribution of the cryoprotectant among fish cells and a uniform freezing process.…”

Section: Introductionmentioning

confidence: 99%

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Finite Element Modeling of Crystallization with Temperature Jump to Improve Cryopreservation of Fish Germ Cells

Matrosov,

Soloviev,

Ponomareva

et al. 2024

Processes

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This article is devoted to the further development of a viable technology for low-temperature cryopreservation of reproductive cells of sturgeon fish using acoustic–mechanical fields and intelligent control of the freezing process. Before vitrification begins, the piezoactuator acts on a mixture of cryoprotectant and reproductive cells. This promotes intensive mixing of the cryoprotector and its diffusion through the cell membrane. When vitrification is carried out directly, a phase transition phenomenon is observed, accompanied by crystal formation. This article presents a new mathematical model describing this process as developed by the authors. The corresponding boundary conditions are formulated. Numerical experiments were carried out using the finite element method. It has been established that during vitrification without the use of a cryoprotectant, a sharp temperature jump is observed at the front of the crystalline formation boundary. The use of a cryoprotectant leads to a slowdown in the process of crystal formation, that is, to a weakening of the effect of one of the most important cryoprotective factors. The comparison with full-scale experiments showed qualitative agreement with the experimental results, which indicates the adequacy of the proposed model. The results obtained can be used in the future during the vitrification process and the evaluation of the quality of cryofreezing. The application of a new methodological approach to methods of long-term preservation at low temperatures of the genetic and reproductive material of hydrobionts using acoustic and mechanical effects and an intelligent control module opens up great opportunities for the creation of new cost-effective biotechnologies that make it possible to make the transition to a new type of aquatic farms, increase the stability of aquaculture, in general, to make environmental protection measures to save rare and endangered species more effective.

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Section: Discussionmentioning

confidence: 99%

Section: Mathematical Model For Crystallizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Finite Element Modeling of Crystallization with Temperature Jump to Improve Cryopreservation of Fish Germ Cells

Matrosov,

Soloviev,

Ponomareva

et al. 2024

Processes

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“…Although successful vitrification and rewarming of tissue samples with a thickness of 1 mm have been achieved, the conventional rewarming procedure’s physical limitations prevent its application to large-volume biological samples. When rewarming larger biological tissue materials like blood vessels following vitrification, damage from ice crystal formation during devitrification and thermal stress due to uneven temperature distribution directly affect blood vessel cryopreservation, resulting in irreversible damage. , Pegg et al discovered that thermal stress damage to blood vessels primarily occurs during the rewarming stage between −150 and −100 °C, as rapid rewarming can lead to uneven temperature distribution within the solution system. The vessels crack and fracture due to excessive thermal stress.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Annealing and Metal Films Radiofrequency Heating Procedures for Vitrified Umbilical Arteries

Zuo,

Cao,

Han

et al. 2024

Langmuir

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Vitrification is well known for its application in the cryopreservation of blood vessels, which will address the supply− demand imbalance in vascular grafts for the treatment of cardiovascular disease. Thermal stress damage and devitrification injury in umbilical arteries (UAs) require attention and resolution during the vitrification and rewarming process. In this study, we validated several cooling annealing protocols with temperatures (−130 to −100 °C) and annealing duration durations (10−20 s). Among these, the umbilical artery subjected to annealing at −110 °C for 10 s exhibited the most favorable glass transition and retained 93% of its elastic modulus (0.625 ± 0.030 MPa) compared to the fresh group. Extended annealing temperatures and durations can effectively reduce thermal stress damage, leading to improved mechanical properties by minimizing temperature gradients during cooling. Furthermore, three metal radiofrequency methods were utilized for rewarming, including the use of additional metal films and different magnetic field strengths (20, 25 kA/m). Metal radiofrequency (adding an extra metal film for cryoprotectants rewarming, 20 kA/m) achieved faster and more uniform rewarming, preserving the extracellular matrix (ECM), collagen fibers, and elastic fibers without significant differences compared to the fresh group (P < 0.05). Moreover, its preservation of the biomechanical properties of blood vessels was better than that of water bath heating. Theoretical analysis supports these findings, indicating that radiofrequency heating (RFH) with metal films reduces temperature gradients and thermal stresses during arterial rewarming. RFH contributes to the cryopreservation and clinical application of large-lumen biomaterials, overcoming challenges associated with vascular vitrification and rewarming.

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“…Electromagnetic (EM) heating was used for rewarming cryopreserved canine kidneys as early as the 1870s . However, it cannot yet be applied to the uniform heating of large-volume biological samples due to many influencing factors such as the skin effect, fast decay of field strength, and change in the dielectric constant of biomaterials with temperature. ,, In addition, Jin et al − utilized infrared laser pulses to achieve an ultrarapid warming rate (10 7 °C/min) for rewarming frozen or vitrified oocytes. This approach significantly improved survival after the rewarming of frozen oocytes.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Rapid Rewarming Chips for Cryopreservation by Joule Heating

Han,

Zhan,

Cui

et al. 2023

Langmuir

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Rapid and uniform rewarming is critical to cryopreservation. Current rapid rewarming methods require complex physical field application devices (such as lasers or radio frequencies) and the addition of nanoparticles as heating media. These complex devices and nanoparticles limit the promotion of the rapid rewarming method and pose potential biosafety concerns. In this work, a joule heating-based rapid electric heating chip (EHC) was designed for cryopreservation. Uniform and rapid rewarming of biological samples in different volumes can be achieved through simple operations. EHC loaded with 0.28 mL of CPA solution can achieve a rewarming rate of 3.2 × 10 5 °C/min (2.8 mL with 2.3 × 10 3 °C/min), approximately 2 orders of magnitude greater than the rewarming rates observed with an equal capacity straw when combined with laser nanowarming or magnetic induction heating. In addition, the degree of supercooling can be significantly reduced without manual nucleation during the cooling of the EHC. Subsequently, the results of cryopreservation validation of cells and spheroids showed that the cell viability and spheroid structural integrity were significantly improved after cryopreservation. The viability of human lung adenocarcinoma (A549) cells postcryopreservation was 97.2%, which was significantly higher than 93% in the cryogenic vials (CV) group. Similar results were seen in human mesenchymal stem cells (MSCs), with 93.18% cell survival in the EHC group, significantly higher than 86.83% in the CV group, and cells in the EHC group were also significantly better than those in the CV group for further apoptosis and necrosis assays. This work provides an efficient rewarming protocol for the cryopreservation of biological samples, significantly improving the quantity and quality of cells and spheroids postcryopreservation.

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Development of a Novel Electromagnetic Rewarming Technology for the Cryopreservation of Stem Cells with Large Volume

Cited by 5 publications

References 47 publications

Finite Element Modeling of Crystallization with Temperature Jump to Improve Cryopreservation of Fish Germ Cells

Finite Element Modeling of Crystallization with Temperature Jump to Improve Cryopreservation of Fish Germ Cells

Optimization of Annealing and Metal Films Radiofrequency Heating Procedures for Vitrified Umbilical Arteries

Investigation of Rapid Rewarming Chips for Cryopreservation by Joule Heating

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