Development of a Novel Differential Duplex PCR for Pathogenic and Intermediate Leptospires: First Report of Intermediate Leptospira in Animal Sera in Argentina
Abstract:Background: Even though leptospirosis is one of the most important zoonoses in the world, many of its aspects remain unknown, such as the distribution of different species and their pathogenicity for animals and humans. Results: Intermediate leptospiral DNA was detected in bovine sera samples from animals with abortion history from Argentina. We designed a novel duplex PCR, which differentiates pathogenic and intermediate leptospires in one single step. This new diagnostic method allowed the detection of inter… Show more
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