Development of a novel biosensor based on F0F1-ATPase for the detection of 2-dodecylcyclobutanone in irradiated beef

Irradiation of food products and ingredients must be indicated by proper labeling. This study evaluated the appropriateness of the European Standard EN 1785:2003 for the detection of 2-alkylcyclobutanones, which are radiolysis products of fatty acids, in cashew nuts and nutmeg and confirmed its suitability to detect irradiation of cashew nut samples at average absorbed doses of 1 kGy and above. An alternative method was developed, which is based on matrix solid phase dispersion and subsequent separation and detection of oxime derivatives of 2-alkylcyclobutanones by high performance-high resolution mass spectrometry. It is more rapid, less resource consuming, and more sensitive than EN 1785:2003. This method allowed detection of 2-alkylcyclobutanones in cashew nuts irradiated at 100 Gray and in nutmeg irradiated at 400 Gray. None of the 26 cashew nut and 14 nutmeg samples purchased in different EU Member States contained traces of 2-alkylcyclobutanones.

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FOF1-ATP synthase molecular motor biosensor for miRNA detection of colon cancer

Gao

Zhang

Pan

et al. 2023

Life Sciences

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Development of a novel biosensor based on F0F1-ATPase for the detection of 2-dodecylcyclobutanone in irradiated beef

Cited by 7 publications

References 21 publications

New Insights to Detect Irradiated Food: an Overview

New Insights to Detect Irradiated Food: an Overview

Comparative evaluation of methods for the detection of 2-alkylcyclobutanones as indicators for irradiation treatment of cashew nuts and nutmeg

FOF1-ATP synthase molecular motor biosensor for miRNA detection of colon cancer

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