Development of a non-viral multifunctional envelope-type nano device by a novel lipid film hydration method

Non-linear pharmacodynamics in a non-viral gene delivery system: Positive non-linear relationship between dose and transfection efficiency

“…This non-linear increase of luciferase activity at higher value of pDNA was not expected and could also not be explained. A similar result was also observed for the R8-MEND, a recently developed non-viral gene delivery system [6] ( Fig. 1 (B)).…”

“…The MEND was prepared by the lipid film hydration method described in a previous report [6]. In a typical experiment, DNA dissolved, in 10mM HEPES buffer (pH 7.4) (0.1 mg/ml), was mixed with a solution of PLL (0.1 mg/ml) to condense the pDNA under vortexing at a nitrogen/phosphate (N/P) ratio of 2.4.…”

A new peptide motif present in the protective antigen of anthrax toxin exerts its efficiency on the cellular uptake of liposomes and applications for a dual-ligand system

“…CPPs, also known as protein transduction domains (PTDs) have the capability to enter into the cells either alone or in conjugation with small molecules or bulky cargos such as peptides, proteins, oligonucleotides, plasmid DNA (pDNA) or liposomes. In previous studies, we reported on the development of stearylated octaarginine (STR-R8) modified on the surface of 45 liposomes encapsulating plasmid DNA (pDNA), small interfering RNA (siRNA), or proteins, which can be efficiently internalized by cells (Kogure et al, 2004;Nakamura et al, 2007;Suzuki et al, 2007). We recently proposed a dual-ligand liposomal delivery system modified with a specific ligand on the top of the PEG chain and STR-R8, as a CPP ligand, on the surface of 50 liposomes (Takara et al, 2010).…”

Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery

“…Over the years, a number of cell-penetrating peptides derived from HIV-1 Tat protein, Antennapedia protein of Drosophila, and related peptides, such as arginine oligomers, have been studied for possible improvement of the pharmacokinetic properties of drugs with low bioavailability, including peptides [5,6], proteins [7,8], and nucleic acids [9][10][11][12][13]. Different virus-derived arginine-rich peptides can transfect cells with luciferase-encoding plasmids as efficiently as polyarginine and polylysine [9].…”

“…as the primary functional device, we recently developed a multifunctional envelope-type nano-device (MEND) for use as a non-viral gene delivery system [13]. In this system, DNA/polycation cores are encapsulated by a STR-R8-surface modified lipid envelope.…”