Development of a new monoclonal antibody based direct competitive enzyme-linked immunosorbent assay for detection of brevetoxins in food samples

Zhou, Yu; Li, Yansong; Pan, Fengguang; Zhang, Yuanyuan; Lu, Shiying; Ren, Hong-Lin; Li, Zhaohui; Liu, Zengshan; Zhang, Junhui

doi:10.1016/j.foodchem.2009.05.015

Cited by 36 publications

(28 citation statements)

References 13 publications

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“…Titers of antiserum and immunization period ( long period of more than 70 days, which is close to the results of other studies (Cheong et al, 2007;Mesci & Carlyle, 2007;Miyano, Kodama, & Hamakubo, 2006;Naar, Branaaa, Bottein-Dechraoui, Chinain, & Pauillac, 2001;Sheng, He, & Shi, 2007;Xu, Liang, Belisle, & David, 2008;Zhou, Zhang, et al, 2009;Zhou et al, 2010). The footpad immunization and lymph node cell for fusion is suitable for generation of mAbs against protein toxin, abrin.…”

Section: Mouse Nosupporting

confidence: 86%

Development of a monoclonal antibody-based sandwich-type enzyme-linked immunosorbent assay (ELISA) for detection of abrin in food samples

et al. 2012

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Section: Mouse Nosupporting

confidence: 86%

Development of a monoclonal antibody-based sandwich-type enzyme-linked immunosorbent assay (ELISA) for detection of abrin in food samples

et al. 2012

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“…there are also a variety of antibody-based methods (Baden et al, , 1988Fire et al, 2008;levine and Shimizu, 1992;Naar et al, 2002Naar et al, , 1998Plakas et al, 2008;Poli et al, 1995Poli et al, , 2007trainer and Poli, 2000;Zhou et al, 2010). For example, a competitive elISA was established with a lOD of 25 µg Btx/kg oyster (Naar et al, 2002).…”

Section: )mentioning

confidence: 99%

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Daneshian

2013

ALTEX

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Summary Aquatic food accounts for over 40% of global animal food products, and the potential contamination with toxins of algal origin -marine biotoxins -poses a health threat for consumers. The gold standards to assess toxins in aquatic food have traditionally been in vivo methodssingle marine biotoxins or combinations of marine biotoxins and intoxication symptomatology as well as monitoring the "success" of controls implemented during fishery and aquaculture production, processing, and retailing. For this reason, a workshop was organized in ermatingen, Switzerland by the Center for Alternatives to Animal testing -europe (CAAteurope) within the framework of the transatlantic think tank for toxicology (t 4 ).In contrast to other food products where hygiene and potential contamination with microbes or mold toxins is the prime issue, the emphasis in aquatic products (shellfish and finfish) is, beyond viral and bacterial contaminations, primarily placed on potential contamination with marine biotoxins owing to the numerous and recurring human intoxications.The gold standards to assess toxins in aquatic food have traditionally been in vivo methods, i.e., the mouse and the rat bioassays. Besides the ethical issues of in vivo bioassays there are specific difficulties, e.g., different exposure route (i.p. versus oral), low inter-species comparability, high intra-species variability, and questionable extrapolation of quantitative risk to humans, thus highlighting the need for the development of more reliable detection and quantification methods. Based on this reasoning, the european Food Safety Authority (eFSA) advocates the use of an analytical method, i.e., LC-MS (Liquid Chromatography coupled Mass Spectrometry), as a substitute for in vivo bioassays for almost all classes of marine toxins. However, although lC-MS is a very sensitive method that has the advantages of being able to detect multiple toxins in a single analysis and being good for confirming the identity of toxins, the quality of quantitative analysis is dependent on the availability of calibration standards. While many new toxin structural analogues have been detected and identified using LC-MS, this technique -as with most other methods -is not good at detecting new toxin types. When new toxin analogues are detected but accurate standards are not yet available, only an approximate quantitation is possible using estimated response factors.For the purpose of risk assessment of food, however, it is imperative to focus on the possible adverse effects, independent of whether they stem from one toxin or a combination of toxins. As toxicity is defined by functional and structural changes of biological systems, the development of a human-relevant in vitro system for appropriate risk assessment of marine biotoxins in seafood stands to reason. Moreover, poor correlation of human intoxications, of acute symptomatology and long-term adverse effects, and of predictive in vitro, in vivo, and analytical detection methodologies of marine biotoxins stems not least from a...

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“…The emulsion of immunogen (melamine-BSA) and CFA or IFA was made according the methods of Zhou et al (2010). The Balb/C mice were immunised by subcutaneous injection in a hind footpad with 20 lL of the CFA emulsion containing 25 lg of protein.…”

Section: Immunisation Of Micementioning

confidence: 99%

“…Popliteal lymph nodes cell suspension was prepared according to the methods of Zhou et al (2010). Murine myeloma cells (SP2/ 0) were cultured in RPMI 1640 media supplemented with 20% foetal bovine serum and cell fusion procedures were carried out essentially as described by Zhou et al (2010).…”

Section: Hybridoma Screeningmentioning

confidence: 99%

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Monoclonal antibody based inhibition ELISA as a new tool for the analysis of melamine in milk and pet food samples

Zhou¹,

Li²,

Li³

et al. 2012

Food Chemistry

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Development of a new monoclonal antibody based direct competitive enzyme-linked immunosorbent assay for detection of brevetoxins in food samples

Cited by 36 publications

References 13 publications

Development of a monoclonal antibody-based sandwich-type enzyme-linked immunosorbent assay (ELISA) for detection of abrin in food samples

Development of a monoclonal antibody-based sandwich-type enzyme-linked immunosorbent assay (ELISA) for detection of abrin in food samples

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Monoclonal antibody based inhibition ELISA as a new tool for the analysis of melamine in milk and pet food samples

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