Development of a New Method To Prepare Nano-/microparticles Loaded with Extracts of Azadirachta indica, Their Characterization and Use in Controlling Plutella xylostella

Abstract

Biodegradable nanoparticles have been widely explored as carriers for controlled delivery of therapeutic molecules; however, studies describing the development of nanoparticles as carriers for biopesticide products are few. In this work, a new method to prepare nanoparticles loaded with neem (Azadirachta indica) extracts is presented. In this study, nanoparticles were formulated as colloidal suspension and (spray-dried) powder and characterized by evaluating pH, particle size, zeta potential, morphology, absolute recovery, and entrapment efficiency. A high-performance liquid chromatography method was used for nanoparticle characterization. The best formulations presented absolute recovery and entrapment efficiencies of approximately 100% and a release profile based on swelling and relaxation of the polymer or polymer erosion. The biological data of the formulated products against Plutella xylostella showed 100% larval mortality. The nanoparticle information improved the stability of neem products against ultraviolet radiation and increased their dispersion in the aqueous phase.

Use of Lignins from Sugarcane Bagasse for Assembling Microparticles Loaded withAzadirachta indicaExtracts for Use as Neem-Based Organic Insecticides

“…Good results were obtained when the nanoparticles were formulated with neem oil (3.5 g), the F-EtOAc fraction of the neem extract (0.3 g), lignin (1.5 g) and a surfactant (Tween ® 80, 0.67 g), using the nanoemulsion/solvent displacement method and the interfacial deposition nanoencapsulation process. 20 Colloidal silicon dioxide (Aerosil ® 200, 1.5 g) was then added to the colloidal suspension and homogenized with magnetic stirring. This mixture was fed into a spray-dryer (Büchi B-290, Büchi Labortechnik, Switzerland) operated using a 0.7 mm atomizer nozzle, with air as the atomizing gas and a sample feed rate of 0.75 mL min -1 , an atomizing air flow of 530 L h -1 at a 90% capacity aspirator (38 m 3 h -1…”

“…For both assays, the samples were removed successively at specific times and analyzed by HPLC. 20 Efficacy studies using biological assays…”

“…Quantitative analyses were performed by fitting the value of area produced by the analyte azadirachtin (retention time: 10.28 minutes) in each chromatogram in a calibration curve constructed by using linear regression of a set of solutions of azadirachtin standard in six different concentrations, following previously described methodology. 15,20 Chromatograms used in quantification are displayed in Figure 5.…”

Efeito inseticida sistêmico de nanoformulações à base de nim sobre Bemisia tabaci (Hemiptera: Aleyrodidae) biótipo B em tomateiro

“…A quantificação do teor de azadiractina no óleo de nim, no produto comercial utilizado e nas nanoformulações obtidas, foi realizada por meio de cromatografia líquida de alta eficiência acoplada ao detector ultravioleta (HPLC-UV, Agilent Technologies 1260 Series), conforme descrito por Forim et al (2010Forim et al ( , 2013. A faixa linear de trabalho usada para quantificação de azadiractina nas diferentes matrizes foi obtida pela análise de soluções-padrão em concentrações variando de 1 a 100 µg mL -1 .…”

Application Of Nanotechnology In Agriculture And Food Industry, Its Prospects And Risks

“…NPs loaded with neem (Azadirachta indica) extracts formulated as colloidal suspension and (spray-dried) powder showed 100% larval mortality against P. xylostella [178]. A nanogel prepared from the pheromone methyl eugenol using a low-molecular mass gelator was found to be suitable for an effective management of Bactrocera dorsalis [179].…”