Development of a New Isolation Technique and Validated RP-HPLC method for Quercetin and Kaempferol from <i>Azadirachta indica</i> leaves

Prakash, Om; Mahapatra, Debarshi Kar; Singh, Ruchi; Singh, Namrata; Verma, Neelam; Ved, Akash

doi:10.5958/2231-5675.2018.00030.3

Cited by 16 publications

(8 citation statements)

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“…Also, natural antimicrobials such as eugenol, thymol, carvacrol, cinnamaldehyde, and allyl isothiocyanate paired with an antibiotic (ampicillin, tetracycline, penicillin, bacitracin, erythromycin, novobiocin) were found to be highly effective in reducing the resistance of S. aureus 55 . Cefixime, ceftriaxone, ciprofloxacin, cephradine, methicillin, ampicillin, and amoxicillin tested alone were inactive against clinical isolated MRSA, while their combinations with quercetin, kaempferol, and both quercetin and kaempferol demonstrated valuable anti-MRSA activity 56 . In turn, the combination of sanguinarine with streptomycin was inactive against MRSA 57 .…”

Section: Herbal Products and Their Constituents Used Alone And In Com...mentioning

confidence: 98%

“…The methanolic extract of P. granatum demonstrated the potential to either inhibit the EPs NorA or to enhance the influx of the drug in the clinical isolates of MRSA and MSSA 37 . Catechin gallates from C. sinensis effectively reverse MRSA resistance through NorA EP inhibition 56 . Smith et al 134 reported that ferruginol from the cones of Chamaecyparis lawsoniana is an EPI that inhibited the activity of the quinolone resistance pump (NorA), the tetracycline resistance pump, (TetK), and the erythromycin resistance pump (MsrA) in S. aureus.…”

Section: Mechanism Of Action Of Herbal Therapy Against Multidrug-resi...mentioning

confidence: 99%

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Herbal Products and Their Active Constituents Used Alone and in Combination with Antibiotics against Multidrug-Resistant Bacteria

Herman

2022

Planta Med

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The purpose of this review is to summarize the current knowledge acquired on herbal products and their active constituents with antimicrobial activity used alone and in combination with antibiotics against multidrug-resistant bacteria. The most promising herbal products and active constituents used alone against multidrug-resistant bacteria are Piper betle (methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus, extended-spectrum beta-lactamase, Acinetobacter baumannii, Pseudomonas aeruginosa), Glycyrrhiza glabra (methicillin-resistant S. aureus, vancomycin-resistant Enterococcus, P. aeruginosa), and berberine (methicillin-resistant S. aureus, A. baumannii, P. aeruginosa), respectively. The synergistic effect of the combination of herbal products and their active constituents with antibiotics against multidrug-resistant bacteria are also described. These natural antibacterial agents can be promising sources of inhibitors, which can modulate antibiotic activity against multidrug-resistant bacteria, especially as efflux pump inhibitors. Other possible mechanisms of action of herbal therapy against multidrug-resistant bacteria including modification of the bacterial cell wall and/or membrane, inhibition of the cell division protein filamenting temperature sensitive Z-ring, and inhibition of protein synthesis and gene expression, all of which will also be discussed. Our review suggests that combination herbal therapy and antibiotics can be effectively used to expand the spectrum of their antimicrobial action. Therefore, combination therapy against multidrug-resistant bacteria may enable new choices for the treatment of infectious diseases and represents a potential area for future research.

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Section: Herbal Products and Their Constituents Used Alone And In Com...mentioning

confidence: 98%

Section: Mechanism Of Action Of Herbal Therapy Against Multidrug-resi...mentioning

confidence: 99%

Herbal Products and Their Active Constituents Used Alone and in Combination with Antibiotics against Multidrug-Resistant Bacteria

Herman

2022

Planta Med

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“…The limit of detection (LoD) is said to be the lowest possible concentration that can be detected by any sophisticated analytical method; however, it is not always essential to estimate the exact amount of the solute precisely. [13] The given formula determines it: LoD = 3.3 (σ / S) Where σ = standard deviation of response; S = slope of the calibration curve. The slope S may be estimated from the calibration curve of the analyte.…”

Section: Limit Of Detectionmentioning

confidence: 99%

Development and Validation of a Stability Indicating RP-HPLC Method for the Determination of Prucalopride succinate in Bulk and Tablet

Kanthale

Thonte²,

Pekamwar

et al. 2020

Int. J. Pharm. Sci. Drug Res.

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A very simple, precise, economical, accurate, robust, and reproducible reverse phase-high-performance liquid chromatography method along with stability indicating attributes has been developed for estimating of prucalopride succinate (PRU) in both bulk and tablet formulation (PRUVICT 2). The estimation of the solutes was performed on a Grace C18 column of dimension 150 mm × 4.6 mm, 5 μm. PRU was eluted with acetonitrile: 0.02 M potassium dihydrogen phosphate in the ratio of 20:80 v/v in a 10 min isocratic mode at a flow rate of 1 ml/min at 30°C column temperature and monitored at a wavelength of 277 nm. The retention time of PRU was found to be 5.416 minutes. The Q2b validation of the analytical method revealed good linearity over the concentration range 2–12 μg/mL for IVA with r2 of 0.999. The mean recovery % over the three tested ranges of 50%, 100%, and 150% were found to be 100.173%, 99.077%, and 98.575%, respectively. In intra-day variability study, the % RSDs was detected to be 0.754, 1.032, and 0.482 whereas the inter-day variability study demonstrated % RSDs of 0.797, 0.559, and 0.524, respectively. The acid, alkali, boiled water, hydrogen peroxide, dry heat, and UV radiations based stress studies presented the formation of a variety of characteristic degradation products. The developed analytical method may be employed for the routine analysis of PRU in bulk and tablet formulations.

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“…The reproducibility profile of the analytical method was estimated by injecting five-times the standard solution and recording the parameters such as retention time, peak area, theoretical plates, and tailing factor (Prakash et al, 2018).…”

Section: Systems Suitability Parametersmentioning

confidence: 99%

Stability indicating RP-HPLC method for the simultaneous estimation of ivabradine and metoprolol in bulk and tablet formulation

2019

J App Pharm Sci

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A new, accurate, precise, and robust reverse phase-high-performance liquid chromatography method has been developed along with sensitive stability indicating attributes for the simultaneous estimation of ivabradine (IVA) and metoprolol (MET) in both bulk and tablet formulation. The estimation of the solutes was performed on a Denali C 18 column of dimension 150 mm × 4.6 mm, 5 μm. IVA and MET were eluted with orthophosphoric acid (0.1%) buffer: acetonitrile in the ratio of 60:40 V/V in a 6 minutes isocratic trial at a flow rate of 0.8 ml/minute having an ambient column temperature of 30°C and monitored at 260 nm wavelength. The retention time of IVA and MET was found to be 2.290 and 3.520 minutes, respectively. The Q2b validation of the analytical method revealed good linearity over the concentration range 5-30 μg/ml for IVA and 25-150 μg/ml for MET with r 2 of 0.999 in both the cases. The mean recovery % over the three tested ranges of 50%, 100%, and 150% were found to be 100.46%, 99.68%, and 99.68%, respectively. The acid, alkali, dry heat, and peroxide-induced stress studies presented the formation of a variety of degradation products. The developed analytical method was precise, accurate, and robust for the routine analysis of the drug combination in bulk and tablet formulations.

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Development of a New Isolation Technique and Validated RP-HPLC method for Quercetin and Kaempferol from Azadirachta indica leaves

Cited by 16 publications

References 0 publications

Herbal Products and Their Active Constituents Used Alone and in Combination with Antibiotics against Multidrug-Resistant Bacteria

Herbal Products and Their Active Constituents Used Alone and in Combination with Antibiotics against Multidrug-Resistant Bacteria

Development and Validation of a Stability Indicating RP-HPLC Method for the Determination of Prucalopride succinate in Bulk and Tablet

Stability indicating RP-HPLC method for the simultaneous estimation of ivabradine and metoprolol in bulk and tablet formulation

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