Development of a new HPLC method for the determination of inorganic and methylmercury in biological samples with ICP-MS detection

Vallant, Birgit; Kadnar, Rainer; Goessler, Walter

doi:10.1039/b615463h

Cited by 70 publications

(44 citation statements)

References 13 publications

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“…wave atomic absorption spectroscopy (AAS), gas chromatography-mass spectroscopy (GC-MS), inductively coupled plasma mass spectroscopy (ICP-MS), and highperformance liquid chromatography (HPLC) (Puk and Weber 1994;Vallant et al 2007;Barin et al 2014;Han et al 2006). However, these techniques are highly expensive, and require sophisticated operating facilities, complex sample treatments and skilled personnel, and thus are not suitable for field deployment.…”

Section: Introductionmentioning

confidence: 99%

Dual mechanism-based sensing of mercury using unmodified, heteroepitaxially synthesized silver nanoparticles

et al. 2017

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Mercury and its compounds are widely distributed in the environment and have a significant negative impact on human health. In this paper, we report the development of a rapid and facile method for the detection of mercury ions (Hg 2? ) using heteroepitaxially synthesized unmodified silver nanoparticle-based smart probes using UV-Vis spectrophotometer and also through the naked eye by means of a paper-based sensor strip. The silver nanoparticles were prepared by heteroepitaxial growth method using gold seed nanoparticle of *2.4 nm size as the template. The silver is grown on the seed particles by reducing the silver-ammonia complex using glucose, which resulted in Glu-AgNPs having an average size of 14.65 ± 3.53 nm. The sensing of mercury ions was carried out in aqueous solution and the reaction response was monitored by UV-Vis spectrophotometer. The interaction of Hg 2? with Glu-AgNPs resulted in a significant drop in the absorbance at 402 nm along with a prominent color change (from bright yellow to colorless) and wavelength shift (blue shift). The limit of detection (LOD) of this assay was found to be 100 nM (i.e., 20 ppb) with a good linearity in the concentration range of 100-10 mM. To further ease the detection process and make it field deployable, we attempted to develop a paper-based sensor strip by immobilizing Glu-AgNPs on a paper strip. Upon interaction with mercury solution of varying concentrations, the decoloration of the spots could be observed easily through naked eyes, with the limit of detection under sub-optical conditions being 1 lM.

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Section: Introductionmentioning

confidence: 99%

Dual mechanism-based sensing of mercury using unmodified, heteroepitaxially synthesized silver nanoparticles

et al. 2017

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“…Moreover, these works all involved a very complex synthesis of fluorescent materials. As far as we know, some other analytical methods have been developed for the determination of mercury, such as electrochemical method [20][21][22][23][24][25][26], atomic absorption spectrometry [27][28][29][30] or cold-vapour inductively coupled plasma atomic emission [31], chromatographic determination [32][33][34], inductively coupled plasma-mass spectrometric analysis [35,36] or electrospray ionization mass spectrometry [37], room temperature phosphorimetry [38]. Although these methods are accurate, most require a tedious sample pre-treatment, sophisticated performance and/or expensive equipment.…”

Section: Introductionmentioning

confidence: 99%

An optical sensor for mercury ion based on the fluorescence quenching of tetra(p-dimethylaminophenyl)porphyrin

Yang

Jiang

Shen

et al. 2009

Analytica Chimica Acta

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a b s t r a c tAn optical sensor for mercury ion (Hg 2+ ), based on quenching the fluorescence of the sensing reagent porphyrin immobilized in plasticized poly(vinyl chloride) (PVC) membrane, has been developed. The responses to mercury ion were compared for the sensors modified with three porphyrin compounds including 5,10,15,20-tetraphenylporphyrin (TPP), tetra(p-dimethylaminophenyl)porphyrin (TDMAPP) and tetra(N-phenylpyrazole) porphyrin (TPPP). Among them, TDMAPP showed the most remarkable response to Hg 2+ . The drastic decrease of the TDMAPP fluorescence intensity was attributed to the formation of a complex between TDMAPP and Hg 2+ , which has been utilized as the fabrication basis of a Hg 2+ -sensitive fluorescence sensor. The analytical performance characteristics of the TDMAPP modified sensor was investigated. The response mechanism, especially involving the response difference of three porphyrin compounds, was discussed in detail. The sensor can be applied to the quantification of Hg 2+ with a linear range covering from 4.0 × 10 −8 mol L −1 to 4.0 × 10 −6 mol L −1 . The limit of detection was 8.0 × 10 −9 mol L −1 . The sensor exhibited excellent reproducibility, reversibility and selectivity. Also, the TDMAPP-based sensor was successfully used for the determination of Hg 2+ in environmental water samples.

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“…Vallant et al (2007) suggested ultrasonic extraction with 5 mol/l hydrochloric acid as a sample treatment for LC-ICP-MS analysis. Therefore, we applied partial hydrolysis using 5 mol/l HCl at an elevated temperature followed by 2-mercaptoethanol extraction (procedure B).…”

Section: Chromatographic Separation Of Mercury Speciesmentioning

confidence: 99%

“…methylmercury and inorganic divalent mercury), manage without any derivatisation. Mobile phases usually contain some thiol compounds, most commonly 2-mercaptoethanol (Huang & Jiang 1993;Ramalhosa et al 2001;Vidler et al 2007;Lin et al 2008;Weiyue et al 2011), cysteine (Wan et al 1997;Hight & Cheng 2006;Percy et al 2007;Vallant et al 2007;Sannac et al 2009) or both these compounds (Chiou 2001;Rodrigues et al 2010). The thiol compounds are also used as components of extractants to isolate mercury species from solid samples (Chiou et al 2001;Hight & Cheng 2006;Wang et al 2007;Sannac et al 2009;Rodrigues et al 2010;de Souza et al 2010;Weiyue et al 2011).…”

mentioning

confidence: 99%

Determination of mercury species in foodstuffs using LC-ICP-MS: the applicability and limitations of the method

Koplík¹,

Klimešová²,

Mališová³

et al. 2014

Czech J. Food Sci.

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Koplík R., Klimešová I., Mališová K., Mestek O. (2014): Determination of mercury species in foodstuffs using LC-ICP-MS: the applicability and limitations of the method. Czech J. Food Sci., 32: 249-259.Reversed-phase liquid chromatography hyphenated with inductively coupled plasma mass spectrometry (LC-ICP-MS) was used for mercury speciation analysis in food samples. A short chromatographic column (Purospher ® RP-8e, 75 × 4 mm, 3 µm) and a mobile phase containing 0.02 mol/l CH 3 COONH 4 + 0.2% (v/v) 2-mercaptoethanol (2-ME) + 1% (v/v) CH 3 OH were applied. A repeated extraction of samples with hydrochloric acid/2-ME solution (1 mol/l HCl + 0.2% (v/v) 2-ME) was applied as the isolation step. The results were satisfactory for most food matrices (fish, shellfish, plant materials). Conversely, to analyse high-protein animal matrices, which contain mostly the inorganic form of mercury, a procedure including partial hydrolysis using hydrochloric acid should be used. For methylmercury and inorganic divalent mercury, the LOQ values of 0.3 and 2 ng/g, respectively, can be achieved if precautionary measures against contamination are fulfilled. The method was applied for the determination of methylmercury and inorganic divalent mercury in fish, vegetables, herbs and cereal products.

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Development of a new HPLC method for the determination of inorganic and methylmercury in biological samples with ICP-MS detection

Cited by 70 publications

References 13 publications

Dual mechanism-based sensing of mercury using unmodified, heteroepitaxially synthesized silver nanoparticles

Dual mechanism-based sensing of mercury using unmodified, heteroepitaxially synthesized silver nanoparticles

An optical sensor for mercury ion based on the fluorescence quenching of tetra(p-dimethylaminophenyl)porphyrin

Determination of mercury species in foodstuffs using LC-ICP-MS: the applicability and limitations of the method

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