In this paper, the effect of testing temperature on the performance of fixed carrier membrane for CO 2 separation were studied. The blend composite membranes were developed respectively with a blend of PEI-PVA (polyetheleneimine-polyvinyl alcohol) as separation layer and PS (polysulfone) ultrafiltration membranes as the substrates. The permselectivity of the membranes was measured with CO 2 /CH 4 mixed gas. The effect of testing temperature on membrane separation performance was investigated. The results showed that both the permeances of CO 2 and CH 4 decreased with the increase of temperature, and the permeances decreased more quickly under low pressure than those under high pressure. At the feed pressure of 0.11 MPa, the CO 2 / CH 4 selectivity of PEI-PVA/PS blend composite membrane reduced along with temperature increment. Under the feed pressure of 0.21 MPa, as well as 1.11 MPa, the selectivity decreased with the increase of temperature.