Development of a new electroconductive nanofibrous cardiac patch based on polyurethane-reduced graphene oxide nanocomposite scaffolds

Shabankareh, Azar Najafi Tireh; Pakchin, Parvin Samadi; Hasany, Masoud; Ghanbari, Hossein

doi:10.1016/j.matchemphys.2023.127961

Cited by 6 publications

(4 citation statements)

References 63 publications

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“…The low rGO concentrations can enhance membrane mechanics, while higher concentrations may weaken ultimate tensile strength due to rGO aggregation. 18,39,59,83 The initial modulus of the XD PU-10%rGO membrane was comparable with that of healthy myocardium, ranging from 0.02 to 0.5 MPa. 80,84 Native myocardium demonstrates adaptiveness to prolonged stress through collagen and elastin's conformational changes, allowing it to unfurl and withstand stress.…”

Section: ■ Discussionmentioning

confidence: 74%

“…58 In contrast, the blending of rGO with synthetic polymers, such as PLCL and thermoplastic PU, is less frequently explored in the literature. 21,38,39 This research diverges from the currently utilized nonbiodegradable commercial thermoplastic PU (Tecoflex SG-80A) rGO membrane. 38,39 We explored a biodegradable elastic PU blended with rGO with comprehensive mechanical analysis and NRCMs and hiPSC-CMs culture and functional measurement, which have not been performed before.…”

Section: ■ Discussionmentioning

confidence: 99%

“…17,34−37 Despite these established benefits of PU in cardiac patch applications, the integration of rGO into PU-based membranes to form a conductive material represents a less explored avenue. 38,39 Previous research reported combining nonbiodegradable PU with rGO for cardiomyocyte culture in vitro and did not fully explore their mechanical and electrical properties and cardiomyocyte behavior on the membrane. 38,39 This work would address the gap by combining biodegradable elastic PU with rGO to generate a conductive fibrous composite with the aim to systematically investigate cardiomyocyte behaviors on the conductive elastic membranes that mimic the native myocardium.…”

Section: ■ Introductionmentioning

confidence: 99%

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Reduced Graphene-Oxide-Doped Elastic Biodegradable Polyurethane Fibers for Cardiomyocyte Maturation

Taylor,

Xu,

Rogozinski

et al. 2024

ACS Biomater. Sci. Eng.

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Conductive biomaterials offer promising solutions to enhance the maturity of cultured cardiomyocytes. While the conventional culture of cardiomyocytes on nonconductive materials leads to more immature characteristics, conductive microenvironments have the potential to support sarcomere development, gap junction formation, and beating of cardiomyocytes in vitro. In this study, we systematically investigated the behaviors of cardiomyocytes on aligned electrospun fibrous membranes composed of elastic and biodegradable polyurethane (PU) doped with varying concentrations of reduced graphene oxide (rGO). Compared to PU and PU-4%rGO membranes, the PU-10%rGO membrane exhibited the highest conductivity, approaching levels close to those of native heart tissue. The PU-rGO membranes retained anisotropic viscoelastic behavior similar to that of the porcine left ventricle and a superior tensile strength. Neonatal rat cardiomyocytes (NRCMs) and human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) on the PU-rGO membranes displayed enhanced maturation with cell alignment and enhanced sarcomere structure and gap junction formation with PU-10%rGO having the most improved sarcomere structure and CX-43 presence. hiPSC-CMs on the PU-rGO membranes exhibited a uniform and synchronous beating pattern compared with that on PU membranes. Overall, PU-10%rGO exhibited the best performance for cardiomyocyte maturation. The conductive PU-rGO membranes provide a promising matrix for in vitro cardiomyocyte culture with promoted cell maturation/functionality and the potential for cardiac disease treatment.

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Section: ■ Discussionmentioning

confidence: 74%

Section: ■ Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reduced Graphene-Oxide-Doped Elastic Biodegradable Polyurethane Fibers for Cardiomyocyte Maturation

Taylor,

Xu,

Rogozinski

et al. 2024

ACS Biomater. Sci. Eng.

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“…3(c) shows a comparison between the conductivity in our work with that of other ion-conducting materials, and the results show that the present material has a relatively higher conductivity than other similar materials. 47–53…”

Section: Resultsmentioning

confidence: 99%

High ionic conductive, freezing-resistant and transparent polyurethane based on a novel metal ionic deep eutectic solvent

Du,

Zhu,

Yang

et al. 2024

J. Mater. Chem. C

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Graphene Oxide‐Incorporated Polylactic Acid/Polyamidoamine Dendrimer Electroconductive Nanocomposite as a Promising Scaffold for Guided Tissue Regeneration

Koeini,

Solouk,

Akbari

2024

Macro Materials & Eng

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In the recent years, electroconductive scaffolds have shown promising capabilities in guided regeneration of electroactive tissues including nerve, heart muscle, bone, cartilage, and skin. Herein, the fabrication of a novel electroconductive poly (L‐lactic acid) (PLLA)/polyamidoamine (PAMAM) dendrimer nanofibrous scaffold containing graphene oxide (GO) nanosheets is described. The presence of PAMAM with amine terminal groups successfully aminolyzed PLLA. Interestingly, both PAMAM (5% w/w) and GO (0.5, 1, 2% w/w) not only contributed to reducing the fiber diameter, increasing the hydrophilicity and degradation rate, but also provided a nanocomposite scaffold with enhancement in electrical conductivity. By incorporating 1% w/w of GO, the nanocomposite scaffold exhibited optimized properties, including electrical conductivity (≈3.09 × 10−5 S m−1), crystallinity (≈ 47%), Young's modulus (≈16.95 MPa), as well as strength (≈1.58 MPa). This nanocomposite also demonstrated significant antibacterial activity of ≥ 90% against both gram‐positive and gram‐negative bacteria. Cellular assays confirmed acceptable cytocompatibility of the nanocomposite scaffolds containing GO and PAMAM, which can support the viability and proliferation of PC‐12 cells. In conclusion, the presence of GO nanosheets alongside PAMAM dendrimers can synergically promote the properties of the prepared nanofibrous mats which can be used as potential electroconductive scaffolds for guided tissue regeneration.

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Development of a new electroconductive nanofibrous cardiac patch based on polyurethane-reduced graphene oxide nanocomposite scaffolds

Cited by 6 publications

References 63 publications

Reduced Graphene-Oxide-Doped Elastic Biodegradable Polyurethane Fibers for Cardiomyocyte Maturation

Reduced Graphene-Oxide-Doped Elastic Biodegradable Polyurethane Fibers for Cardiomyocyte Maturation

High ionic conductive, freezing-resistant and transparent polyurethane based on a novel metal ionic deep eutectic solvent

Graphene Oxide‐Incorporated Polylactic Acid/Polyamidoamine Dendrimer Electroconductive Nanocomposite as a Promising Scaffold for Guided Tissue Regeneration

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