Development of a new culture medium and efficient protocol for in vitro micropropagation of Ceratonia siliqua L.

Lozzi, Assia; Abdelwahd, Rabha; Mentag, Rachid; Abousalim, Abdelhadi

doi:10.1007/s11627-019-09990-6

Cited by 17 publications

(11 citation statements)

References 68 publications

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“…However, several limitations including batch-to-batch inconsistency, limited availability, presence of impurities, and inhibition of growth have been recorded on the use of agar in the culture medium (Puchooa et al 1999 ). In addition, agar is the most expensive component in plant tissue culture medium and is responsible for around 70–80% of the total cost (Lozzi et al 2019 ). On the other hand, phytagel which is a natural polysaccharide has also been used as a gelling agent in shoot proliferation of W. somnifera (Sivanandhan et al 2011 , 2015 ).…”

Section: Critical Factors Influencing Micropropagationmentioning

confidence: 99%

“…The elimination of gelling agents which saves substantial cost has been tried by many researchers in various plant species (Malik et al 2016 ; Singh 2018 ; Vaidya et al 2019 ; Wangdi and Sarethy 2016 ). The liquid culture system has a number of potential benefits including (i) improved plant growth due to increased availability of nutrients and growth regulators, (ii) adequate aeration in a liquid medium which results in the improvement of shoot growth and multiplication, (iii) sterilization by microfiltration, (iv) addition of fresh media without changing the container, and (v) high efficiency of transferring plants to their natural conditions (Lozzi et al 2019 ; Sivanandhan et al 2013 ). There are very few reports available on the liquid culture system in W. somnifera (Ray and Jha 2001 ; Sivanandhan et al 2013 ).…”

Section: Critical Factors Influencing Micropropagationmentioning

confidence: 99%

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Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal

Kaur

Dolker

Behera

et al. 2022

Plant Cell Tiss Organ Cult

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Withania somnifera (L.) Dunal is a valuable medicinal plant in the Solanaceae family. It is commonly known as Ashwagandha and is widely distributed around the globe. It has multiple pharmacological properties owing to the existence of diverse secondary metabolites viz., withanolide A, withanolide D, withaferin A, and withanone. It is in great demand in the herbal industry because of its extensive use. In this background, the major challenge lies in the rapid multiplication of elite cultivars of W. somnifera in order to produce genetically and phytoconstituents uniform plant material for pharmaceutical industries. Thus it is necessary to explore various biotechnological approaches for the clonal mass propagation and synthesis of pharmaceutically important constituents in W. somnifera . Though there are several studies on in vitro propagation on W. somnifera , yet many factors that critically influence the in vitro response and withanolides production need to be fine-tuned in the pretext of the existing knowledge. The current review focuses on the advancements and prospects in biotechnological interventions to meet the worldwide demands for W. somnifera and its bioactive compounds. This update on in vitro studies on W. somnifera will be useful to many researchers, entrepreneurs, and herbal industries looking for its in vitro mass multiplication and scientific utilization.

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Section: Critical Factors Influencing Micropropagationmentioning

confidence: 99%

Section: Critical Factors Influencing Micropropagationmentioning

confidence: 99%

Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal

Kaur

Dolker

Behera

et al. 2022

Plant Cell Tiss Organ Cult

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“…Cotyledons were aseptically excised from seeds and segmented into portions of 4 -6 mm in length. Leaf, hypocotyl, epicotyls, and root segments were cut from two-month-old seedlings, germinated on the carob culture medium (LAC) that we have developed and successfully applied in our previous research on carob micropropagation (Lozzi et al 2019). All explants (cotyledons, leaves, hypocotyls, epicotyls, and roots) were inoculated on the LAC medium supplemented with 5 µM 2,4dichlorophenoxyacetic acid (2,4-D), 3 % (w/v) sucrose and 0.7 % (w/v) agar.…”

Section: Methodsmentioning

confidence: 99%

Polyphenol contents and antioxidant activity of carob callus cultures

Lozzi¹,

Mentag²,

Alami-Halimi³

et al. 2021

Nova Biotechnol. Chim.

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Carob (Ceratonia siliqua L.) is an important Mediterranean plant species with worldwide commercial and medicinal uses. The establishment of a callus culture protocol as an alternative system to produce polyphenols of chemical and pharmaceutical interest was made in the present study for the first time in carob. Explant type and the light regime are two important factors that influence morphogenic responses and biochemical production. Maximal callus induction (100 %) and biomass accumulation were obtained in cotyledon explants under both tested light regimes (16-hour photoperiod and darkness). However, leaf callus produced a higher amount of polyphenols (TPC) and flavonoids (TFC) but a lower amount of total condensed tannins (TCT) as compared to cotyledon callus. Light treatment has significantly increased TCT content but decreased the antioxidant activity in carob callus cultures. Strong and positive correlations were obtained between TPC and TFC and the antioxidant activities with correlation coefficients ranging between 0.68 and 0.98. The obtained results indicate that calli of C. siliqua have the potential for enhanced production of phenolic compounds with antioxidant activity that is favored by culture under dark condition.

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“…Although many species grow well in these basal salts, there is great interspecific and intra-specific variation in performance on the media [ 9 , 10 , 11 ]. Several studies found successful methods of calculating new basal salt formulations based on the mineral analysis of field tissue, though each method is distinctly different [ 12 , 13 , 14 , 15 , 16 ]. Monteiro et al [ 12 ] found that passionfruit ( Passiflora edulis ) grown in vitro showed frequent leaf bleaching and reduced growth.…”

Section: Introductionmentioning

confidence: 99%

Development of a Micropropagation Protocol for the Ex Situ Conservation of Nuttall’s Scrub Oak (Quercus dumosa)

Ree,

Powell,

Folgado

et al. 2024

Plants

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Worldwide, oak species are threatened with extinction due to habitat loss, pathogens, and changing fire regimes. Ex situ conservation through tissue culture may protect the remaining genetic diversity of Quercus dumosa, or the coastal sage scrub oak, from further loss. We designed three basal salt formulations based on the mineral composition of shoot tips and first leaves from mature Q. dumosa and explored carbohydrate source, stress-mitigating compounds, and plant growth regulator concentrations to develop a method of cultivating many Q. dumosa culture lines in vitro. All three novel basal salt formulations led to decreased necrosis compared with commercial basal salt formulas WPM, MS, and DKW. Substitution of 30 g L−1 sucrose with glucose and adding 250 mg L−1 ascorbic acid, 5.2 mg L−1 SNP sodium nitroprusside, and 103 mg L−1 y-aminobutyric acid improved culture health overall. In an experiment involving 115 culture lines, 0.66 mg L−1 6-benzylaminopurine produced the highest average shoots per explant, but 0.33 mg L−1 produced the greatest proportion of shoots 2 cm or greater. Incubation for 24 h in 20 mg L−1 indole-3-butyric acid led to the most rooting. These methods show promise for the ex situ conservation of many genotypes of endangered Q. dumosa.

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Development of a new culture medium and efficient protocol for in vitro micropropagation of Ceratonia siliqua L.

Cited by 17 publications

References 68 publications

Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal

Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal

Polyphenol contents and antioxidant activity of carob callus cultures

Development of a Micropropagation Protocol for the Ex Situ Conservation of Nuttall’s Scrub Oak (Quercus dumosa)

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