Development of a new comprehensive and reliable endometrial receptivity map (ER Map/ER Grade) based on RT-qPCR gene expression analysis

Enciso, María; Carrascosa, José P.; Sarasa, Jonás; Martínez-Ortiz, Pedro A.; Munné, Santiago; Horcajadas, José A.; Aizpurúa, Jon

doi:10.1093/humrep/dex370

Cited by 86 publications

(74 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the utility of ERA in the clinical practice is still debated [203]. More recently, a smaller set of genes has been proposed to assess the receptivity status of the endometrium in biopsies obtained in the secretory phase [204]. It is reasonable to foresee new additional advances in this area, that is of potential great clinical utility in the management of infertile women undergoing IVF, as well as in women with RIF and unexplained RPL.…”

Section: Endometrial Receptivity Arraymentioning

confidence: 99%

Molecular Signaling Regulating Endometrium–Blastocyst Crosstalk

Massimiani

Lacconi

Civita

et al. 2019

IJMS

129

View full text Add to dashboard Cite

Implantation of the embryo into the uterine endometrium is one of the most finely-regulated processes that leads to the establishment of a successful pregnancy. A plethora of factors are released in a time-specific fashion to synchronize the differentiation program of both the embryo and the endometrium. Indeed, blastocyst implantation in the uterus occurs in a limited time frame called the “window of implantation” (WOI), during which the maternal endometrium undergoes dramatic changes, collectively called “decidualization”. Decidualization is guided not just by maternal factors (e.g., estrogen, progesterone, thyroid hormone), but also by molecules secreted by the embryo, such as chorionic gonadotropin (CG) and interleukin-1β (IL-1 β), just to cite few. Once reached the uterine cavity, the embryo orients correctly toward the uterine epithelium, interacts with specialized structures, called pinopodes, and begins the process of adhesion and invasion. All these events are guided by factors secreted by both the endometrium and the embryo, such as leukemia inhibitory factor (LIF), integrins and their ligands, adhesion molecules, Notch family members, and metalloproteinases and their inhibitors. The aim of this review is to give an overview of the factors and mechanisms regulating implantation, with a focus on those involved in the complex crosstalk between the blastocyst and the endometrium.

show abstract

Section: Endometrial Receptivity Arraymentioning

confidence: 99%

Molecular Signaling Regulating Endometrium–Blastocyst Crosstalk

Massimiani

Lacconi

Civita

et al. 2019

IJMS

129

View full text Add to dashboard Cite

show abstract

“…For decades, plenty of studies have examined the biosynthetic pathway of P. somniferum [7][8][9][10]. With the development of sequencing technology, clarifying the biosynthetic pathway of this plant has become increasingly simple and convenient [11,12]. Additionally, another popular technology, RT-qPCR, is inclusively used for gene expression research due to its quantitative accuracy, high sensitivity, and high-throughput capabilities [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Selection of the Reference Gene for Expression Normalization in Papaver somniferum L. under Abiotic Stress and Hormone Treatment

Zhang

et al. 2020

Genes

View full text Add to dashboard Cite

Papaver somniferum L. is an important medical plant that produces analgesic drugs used for the pain caused by cancers and surgeries. Recent studies have focused on the expression genes involved in analgesic drugs biosynthesis, and the real-time quantitative polymerase chain reaction (RT-qPCR) technique is the main strategy. However, no reference genes have been reported for gene expression normalization in P. somniferum. Herein, nine reference genes (actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), cyclophilin 2 (CYP2), elongation factor 1-alpha (EF-1α), glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic (GAPC2), nuclear cap-binding protein subunit 2 (NCBP2), protein phosphatase 2A (PP2A), TIP41-like protein (TIP41), and tubulin beta chain (TUB)) of P. somniferum were selected and analyzed under five different treatments (cold, drought, salt, heavy metal, and hormone stress). Then, BestKeeper, NormFinder, geNorm, and RefFinder were employed to analyze their gene expression stability. The results reveal that NCBP2 is the most stable reference gene under various experimental conditions. The work described here is the first report regarding on reference gene selection in P. somniferum, which could be used for the accurate normalization of the gene expression involved in analgesic drug biosynthesis.

show abstract

“…Polymeric immunoglobulin receptor activity and interferon-gamma receptor binding were found to be enriched in IFLMN, with polymeric immunoglobulin receptor (PIGR) and interferon gamma (IFNG) (Figure 2 B). The recent transcriptional profile study on endometrial receptivity proved that immunological activity and vascular proliferation belong to the most over-expressed biological functions during window of implantation (WOI) 28 . The expression of molecules with immune function changes during WOI 29 .…”

Section: Resultsmentioning

confidence: 99%

Construction of implantation failure related lncRNA-mRNA network and identification of lncRNA biomarkers for predicting endometrial receptivity

Feng¹,

Shen²,

Lv³

et al. 2018

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Insufficient endometrial receptivity is a major factor leading to implantation failure (IF), and the traditional way of morphological observation of endometrium cannot determine the condition of receptivity sufficiently. Considering that long-noncoding RNAs (lncRNAs) regulate endometrial receptivity and competing endogenous RNA (ceRNA) mechanism works in plenty of biological processes, ceRNA is likely to function in the pathology of IF. In the present study, we aim to construct an implantation failure related lncRNA-mRNA network (IFLMN), and to identify the key lncRNAs as the candidates for predicting endometrial receptivity. The global background network was constructed based on the presumed lncRNA-miRNA and miRNA-mRNA pairs obtained from lncRNASNP and miRTarBase. Differentially expressed genes (DEGs) of IF were calculated using the data of GSE26787, and then re-annotated as differentially expressed mRNAs (DEMs) and lncRNAs (DELs). IFLMN was constructed by hypergeometric test, including 255 lncRNA-mRNA pairs, 10 lncRNAs, and 212 mRNAs. Topological analysis determined the key lncRNAs with the highest centroid. Functional enrichment analyses were performed by unsupervised clustering, GO classification, KEGG pathway, and co-expression module analyses, achieving six key lncRNAs and their ceRNA sub-networks, which were involved in immunological activity, growth factor binding, vascular proliferation, apoptosis, and steroid biosynthesis in uterus and prepared endometrium for embryo implantation. Sixteen endometrial samples were collected during mid-luteal phase, including 8 recurrent implantation failure (RIF) or recurrent miscarriage (RM) women and 8 controls who conceived successfully. Quantitative real-time PCR was performed to compare the expression of the above six lncRNAs, which validated that the expression of all these lncRNAs was significantly elevated in endometrium of RIF/RM patients. Further studies are needed to investigate the underlying mechanism, and the lncRNAs may be developed into predictive biomarkers for endometrial receptivity.

show abstract

Development of a new comprehensive and reliable endometrial receptivity map (ER Map/ER Grade) based on RT-qPCR gene expression analysis

Abstract: The authors have no potential conflict of interest to declare. No external funding was obtained for this study.

Cited by 86 publications

References 43 publications

Molecular Signaling Regulating Endometrium–Blastocyst Crosstalk

Molecular Signaling Regulating Endometrium–Blastocyst Crosstalk

Selection of the Reference Gene for Expression Normalization in Papaver somniferum L. under Abiotic Stress and Hormone Treatment

Construction of implantation failure related lncRNA-mRNA network and identification of lncRNA biomarkers for predicting endometrial receptivity

Contact Info

Product

Resources

About