Development of a New Biofertilizer with a High Capacity for N2Fixation, Phosphate and Potassium Solubilization and Auxin Production

Leaungvutiviroj, Chaveevan; Ruangphisarn, Pimtida; Hansanimitkul, Pikul; Shinkawa, Hidenori; Sasaki, Kohsuke

doi:10.1271/bbb.90898

Cited by 45 publications

(25 citation statements)

References 15 publications

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“…The development of biological approaches to improve biofertilizers represents a unique opportunity for future generations to lower the potential economic costs and environmental impacts of current fossil-fuel-dependent industrial methods for the production of ammonia-derived fertilizers (4)(5)(6). Various laboratories have been investigating the potential of Azotobacter species to provide nitrogen to specific crops for many years, with various degrees of success (5)(6)(7)(8)(9)(10)(11).…”

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confidence: 99%

“…Various laboratories have been investigating the potential of Azotobacter species to provide nitrogen to specific crops for many years, with various degrees of success (5)(6)(7)(8)(9)(10)(11). Although many nitrogen-fixing bacteria produce nitrogen in environments requiring very low oxygen, Azotobacter vinelandii has evolved the capability of fixing nitrogen as a free-living aerobe, despite the fact that nitrogenase is inherently sensitive to oxygen.…”

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Gene Deletions Resulting in Increased Nitrogen Release by Azotobacter vinelandii: Application of a Novel Nitrogen Biosensor

Barney

Eberhart

Ohlert

et al. 2015

Appl Environ Microbiol

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Azotobacter vinelandii is a widely studied model diazotrophic (nitrogen-fixing) bacterium and also an obligate aerobe, differentiating it from many other diazotrophs that require environments low in oxygen for the function of the nitrogenase. As a freeliving bacterium, A. vinelandii has evolved enzymes and transporters to minimize the loss of fixed nitrogen to the surrounding environment. In this study, we pursued efforts to target specific enzymes and further developed screens to identify individual colonies of A. vinelandii producing elevated levels of extracellular nitrogen. Targeted deletions were done to convert urea into a terminal product by disrupting the urease genes that influence the ability of A. vinelandii to recycle the urea nitrogen within the cell. Construction of a nitrogen biosensor strain was done to rapidly screen several thousand colonies disrupted by transposon insertional mutagenesis to identify strains with increased extracellular nitrogen production. Several disruptions were identified in the ammonium transporter gene amtB that resulted in the production of sufficient levels of extracellular nitrogen to support the growth of the biosensor strain. Further studies substituting the biosensor strain with the green alga Chlorella sorokiniana confirmed that levels of nitrogen produced were sufficient to support the growth of this organism when the medium was supplemented with sufficient sucrose to support the growth of the A. vinelandii in coculture. The nature and quantities of nitrogen released by urease and amtB disruptions were further compared to strains reported in previous efforts that altered the nifLA regulatory system to produce elevated levels of ammonium. These results reveal alternative approaches that can be used in various combinations to yield new strains that might have further application in biofertilizer schemes. N utrient requirements are directly linked to biomass production, and any potential increased improvement in the scale of biomass yield will necessitate a proportional increase in the demand for essential nutrients. For all photosynthetic organisms (photoautotrophs such as land plants, algae, and cyanobacteria) with requisite light energy and water, nitrogen is the most limiting and expensive nutrient input for aquaculture and agricultural production alike (1). A majority of our current nitrogen fertilizer production is tied to the burning of fossil fuels to generate ammonia from molecular nitrogen (N 2 gas) through the Haber-Bosch process, which accounts for 3 to 5% of world natural gas consumption, or about 1 to 2% of total worldwide energy expenditures (1-3). In developed countries, industrial nitrogen production is accompanied by a huge economic and energetic cost overall (2), while this key nutrient limits agricultural productivity in developing countries, where energy and infrastructure costs prohibit the utilization of the Haber-Bosch process to produce ammonia from atmospheric nitrogen on a large scale.The development of biological approaches to improve biof...

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confidence: 99%

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confidence: 99%

Gene Deletions Resulting in Increased Nitrogen Release by Azotobacter vinelandii: Application of a Novel Nitrogen Biosensor

Barney

Eberhart

Ohlert

et al. 2015

Appl Environ Microbiol

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“…Por lo tanto, la síntesis y secreción de ácidos orgánicos por los microorganismos acidifica el entorno circundante, lo que, en última instancia, conduce a la liberación de iones potasio del potasio mineral, ya sea por protonación o acidificación. De los diferentes ácidos orgánicos que participan en la solubilización del potasio, el ácido succínico, cítrico, glucónico, α-cetoglucónico y oxálico son los ácidos liberados en mayor cantidad por distintos microorganismos (Leaungvutiviroj et al 2010;Tan et al 2014). …”

Section: Solubilización De Hierro O Producción De Sideróforosunclassified

“…Esto resulta promisorio en la medida que la aplicación combinada de microorganismos podría generar una potenciación o aumento de sus habilidades solubilizadoras (Leaungvutiviroj et al 2010). …”

Section: Conclusionesunclassified

Mecanismos de acción de hongos y bacterias empleados como biofertilizantes en suelos agrícolas: una revisión sistemática

Correa

Pineda-Meneses

Ríos-Osório

2017

Corpoica Cienc. Tecnol. Agropecuaria

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El fósforo, el nitrógeno, el hierro y el potasio son algunos compuestos necesarios para el crecimiento y desarrollo vegetal. Los fertilizantes químicos empleados para aumentar su concentración afectan significativamente el medioambiente y los ecosistemas del suelo. De acuerdo con la literatura científica, los microorganismos con potencial biofertilizante han demostrado poseer diversos mecanismos de acción para solubilizar estos compuestos y así cumplir con los requerimientos de las plantas. La presente revisión sistemática recopila información científica (publicada entre los años 2004 y 2014) que describe los mecanismos de acción de los biofertilizantes microbianos en suelos agrícolas. Se usaron tres bases de datos: ScienceDirect, SpringerLink y Scopus, mediante la ruta de búsqueda (biofertilizer) AND (bacteria OR fungi) AND (effect OR action OR mechanism). Tras la comprobación con diversos criterios de inclusión y exclusión, la búsqueda arrojó un total de 63 artículos originales, incluyendo seis de publicaciones no indexadas. Como resultado de la revisión sistemática, se evidenció que la producción de diversos ácidos orgánicos permite la acidificación del suelo, lo cual facilita la absorción de los elementos. También se pudo observar que la solubilización de fósforo es el mecanismo más frecuentemente descrito, obtenido por Pseudomonas pseudoalcaligenes con hasta 726,5 mg/L de solubilización en este elemento.

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“…This could be one of the reasons for more vulnerability of Pakistani soils to potassium deficiency along with intensive cropping system and injudicious use of potash fertilizers (Mengel 2007). It has been observed that application of potassium fertilizer on such soils often gives good response due to hidden hunger (Leaungvutiviroj et al 2010).…”

Section: Insoluble Potassium Sourcesmentioning

confidence: 99%

Potassium-Solubilizing Bacteria and Their Application in Agriculture

Ahmad

Nadeem

Naveed

et al. 2016

Potassium Solubilizing Microorganisms for Sustainable Agriculture

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Potassium (K) is one of the major macronutrients which play an important role in plant growth and development. Total soil potassium reserves are generally large; however, major portion of it exists in insoluble K minerals and very little potassium becomes available to plants. There are certain microorganisms which use a number of biological processes to make potassium available from unavailable forms. These potassiumsolubilizing bacteria (KSB) can be used as a promising approach to increase K availability in soils, thus playing an important role for crop establishment under K-limited soils. Owing to naturally available source of potassium in soil and high price of synthetic potassium fertilizers, the importance of KSB is increasing day by day. The use of chemical fertilizers can be decreased by using KSB in agriculture that can lead to sustainable agriculture. A number of workers have demonstrated the role of KSB in crop improvement. The present review highlights the importance of KSB for enhancing crop production. The mechanisms used by KSB for K solubilization have been discussed. The work of various scientists regarding plant growth promotion through KSB has been reviewed in detail, and present constraints and future scope of this technology have also been discussed.

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Development of a New Biofertilizer with a High Capacity for N₂Fixation, Phosphate and Potassium Solubilization and Auxin Production

Cited by 45 publications

References 15 publications

Gene Deletions Resulting in Increased Nitrogen Release by Azotobacter vinelandii: Application of a Novel Nitrogen Biosensor

Gene Deletions Resulting in Increased Nitrogen Release by Azotobacter vinelandii: Application of a Novel Nitrogen Biosensor

Mecanismos de acción de hongos y bacterias empleados como biofertilizantes en suelos agrícolas: una revisión sistemática

Potassium-Solubilizing Bacteria and Their Application in Agriculture

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