Development of a new biocathode for a single enzyme biofuel cell fuelled by glucose

Abstract

In this study, we reported the development of Prussian blue (PB), poly(pyrrole-2-carboxylic acid) (PPCA), and glucose oxidase (GOx) biocomposite modified graphite rod (GR) electrode as a potential biocathode for single enzyme biofuel cell fuelled by glucose. In order to design the biocathode, the GR electrode was coated with a composite of PB particles embedded in the PPCA shell and an additional layer of PPCA by cyclic voltammetry. Meanwhile, GOx molecules were covalently attached to the carboxyl groups of PPCA by an amide bond. The optimal conditions for the biocathode preparation were elaborated experimentally. After optimization, the developed biocathode showed excellent electrocatalytic activity toward the reduction of H2O2 formed during GOx catalyzed glucose oxidation at a low potential of 0.1 V vs Ag/AgCl, as well as good electrochemical performance. An electrocatalytic current density of 31.68 ± 2.70 μA/cm2 and open-circuit potential (OCP) of 293.34 ± 15.70 mV in O2-saturated 10 mM glucose solution at pH 6.0 were recorded. A maximal OCP of 430.15 ± 15.10 mV was recorded at 98.86 mM of glucose. In addition, the biocathode showed good operational stability, maintaining 95.53 ± 0.15% of the initial response after 14 days. These results suggest that this simply designed biocathode can be applied to the construction of a glucose-powered single enzyme biofuel cell.

Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer

“…Furthermore, glucose is the substrate in the tests with GOX at a concentration of 80 mM (14,412 ppm). As some authors have detailed (Kausaite-Minkstimiene et al, 2021), glucose can be an inhibitor of glucose oxidase at high concentrations, so we interpret that the inhibition we see in this assay is due to this fact. These results were the basis for the design of the polymeric material.…”

Ultrasensitive photoelectrochemical detection of glutathione based on the multifunctional catalytic properties of phosphotungstic acid

“…Their interference to GSH detection is then eliminated. The electrode potentials of L-cysteine, ascorbic acid, glucose and dopamine were around −0.2 V, −0.08 V, 0.3 V and 0.6 V, [38][39][40][41] respectively. Therefore, in the presence of PTA, L-cysteine and ascorbic acid with lower electrode potential are easily oxidized, while glucose and dopamine remain stable.…”

Recent advances in enzymatic biofuel cells enabled by innovative materials and techniques

“…[52] Recently, Kausaite-Minkstimiene et al also introduced a glucose-powered single enzyme biofuel cell. [64] In their study, F I G U R E  NADH production system coupled with alcohol dehydrogenase for electric production Reproduced with permission. [69] Copyright 2019, American Chemical Society.…”

“…also introduced a glucose‐powered single enzyme biofuel cell. [ 64 ] In their study, a modified graphite rod (GR) cathode comprised of a composite of Prussian blue (PB) and GOx was developed as shown in Figure 6D, possessing the advantages of low cost and reduced complexity of the system. [ 64,65 ] Though the power outputs of these single‐enzyme biofuel cells are still modest when compared to conventional EBFCs (nearly 149 W m −3 , reported by Lang Xu and Fraser A. Armstrong [ 66 ] ), this system can provide us with a more straightforward understanding of enzymatic reaction mechanisms since it proved that intramolecular electron transfer within individual enzyme molecules is an alternative avenue for power generation.…”