Development of a new affinity maturation protocol for the construction of an internalizing anti-nucleolin antibody library
Rita Ribeiro,
João N. Moreira,
João Goncalves
Abstract:Over the last decades, monoclonal antibodies have substantially improved the treatment of several conditions. The continuous search for novel therapeutic targets and improvements in antibody’s structure, demands for a constant optimization of their development. In this regard, modulation of an antibody’s affinity to its target has been largely explored and culminated in the discovery and optimization of a variety of molecules. It involves the creation of antibody libraries and selection against the target of i… Show more
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