Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA–PEG block copolymers

Luu, Yen K.; Kim, K.; Hsiao, Benjamin S.; Chu, Benjamin; Hadjiargyrou, Michael

doi:10.1016/s0168-3659(03)00097-x

Cited by 756 publications

(487 citation statements)

References 39 publications

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“…collagen, silk fibroin, and fibrinogen) and synthetic polymers (e.g. PGA, PLLA, PLGA, and PCL) have been processed into fine nonwoven fabrics for tissue engineering research [48,[50][51][52][53][54][55][56][57][58][59][60]. Various cells have been reported to attach, proliferate, and differentiate into or maintain their functional phenotypes on these electrospun nano-fibrous materials [48,54,58,59,61,62].…”

Section: Electrospinningmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,184

816

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Tissue engineering and regenerative medicine is an exciting research area that aims at regenerative alternatives to harvested tissues for transplantation. Biomaterials play a pivotal role as scaffolds to provide three-dimensional templates and synthetic extracellular-matrix environments for tissue regeneration. It is often beneficial for the scaffolds to mimic certain advantageous characteristics of the natural extracellular matrix, or developmental or would healing programs. This article reviews current biomimetic materials approaches in tissue engineering. These include synthesis to achieve certain compositions or properties similar to those of the extracellular matrix, novel processing technologies to achieve structural features mimicking the extracellular matrix on various levels, approaches to emulate cell-extracellular matrix interactions, and biologic delivery strategies to recapitulate a signaling cascade or developmental/would-healing program. The article also provides examples of enhanced cellular/tissue functions and regenerative outcomes, demonstrating the excitement and significance of the biomimetic materials for tissue engineering and regeneration.

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Section: Electrospinningmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,184

816

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“…Nano-fibrous PLG scaffolds have been formed by electrospinning a polymer solution mixed with plasmid, with the scaffold properties controlled by the composition of the polymer solution and processing parameters [63,64]. A thermally induced phase separation [65] was used to create porous DNA loaded scaffolds with a microcellular pore structure that was dependent upon the quenching temperature and duration, solvent/water ratio, and polymer concentration.…”

Section: Scaffold Encapsulationmentioning

confidence: 99%

Matrices and scaffolds for DNA delivery in tissue engineering

Laporte¹,

Shea²

2007

Advanced Drug Delivery Reviews

240

208

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Regenerative medicine aims to create functional tissue replacements, typically through creating a controlled environment that promotes and directs the differentiation of stem or progenitor cells, either endogenous or transplanted. Scaffolds serve a central role in many strategies by providing the means to control the local environment. Gene delivery from the scaffold represents a versatile approach to manipulating the local environment for directing cell function. Research at the interface of biomaterials, gene therapy, and drug delivery has identified several design parameters for the vector and the biomaterial scaffold that must be satisfied. Progress has been made towards achieving gene delivery within a tissue engineering scaffold, though the design principles for the materials and vectors that produce efficient delivery require further development. Nevertheless, these advances in obtaining transgene expression with the scaffold have created opportunities to develop greater control of either delivery or expression and to identify the best practices for promoting tissue formation. Strategies to achieve controlled localized expression within the tissue engineering scaffold will have broad application to the regeneration of many tissues, with great promise for clinical therapies.

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“…However, when PLGA degrades in vivo, the acidic metabolites can have a detrimental effect on the local pH of the extracellular matrix (ECM), which can cause inflammation and an immune response, or even cell and tissue necrosis (Willerth and Sakiyama-Elbert 2008;Liu et al 2006). Hydrogels prepared from PEG are able to resist protein adsorption due to the non-ionic hydrophilic nature of the polymer (Knop et al 2010) and have been used to engineer a wide range of tissue from bone (Luu et al 2003) and cartilage (Bryant and Anseth 2003) to nerve tissue (Cai and Kim 2010). However, like PLGA, PEG scaffolds often need to be functionalised with matrix ligands or peptides to facilitate cell attachment.…”

Section: Introductionmentioning

confidence: 99%

Surface modified cellulose scaffolds for tissue engineering

et al. 2016

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We report the ability of cellulose to support cells without the use of matrix ligands on the surface of the material, thus creating a two-component system for tissue engineering of cells and materials. Sheets of bacterial cellulose, grown from a culture medium containing Acetobacter organism were chemically modified with glycidyltrimethylammonium chloride or by oxidation with sodium hypochlorite in the presence of sodium bromide and 2,2,6,6-tetramethylpipiridine 1-oxyl radical to introduce a positive, or negative, charge, respectively. This modification process did not degrade the mechanical properties of the bulk material, but grafting of a positively charged moiety to the cellulose surface (cationic cellulose) increased cell attachment by 70% compared to unmodified cellulose, while negatively charged, oxidised cellulose films (anionic cellulose), showed low levels of cell attachment comparable to those seen for unmodified cellulose. Only a minimal level of cationic surface derivitisation (ca 3% degree of substitution) was required for increased cell attachment and no mediating proteins were required. Cell adhesion studies exhibited the same trends as the attachment studies, while the mean cell area and aspect ratio was highest on the cationic surfaces. Overall, we demonstrated the utility of positively charged bacterial cellulose in tissue engineering in the absence of proteins for cell attachment.

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Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA–PEG block copolymers

Cited by 756 publications

References 39 publications

Biomimetic materials for tissue engineering

Biomimetic materials for tissue engineering

Matrices and scaffolds for DNA delivery in tissue engineering

Surface modified cellulose scaffolds for tissue engineering

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