Development of a mutant aerosolized ACE2 that neutralizes SARS-CoV-2
in vivo
Daniel L. Kober,
Marley C. Caballero Van Dyke,
Jennifer L. Eitson
et al.
Abstract:The rapid evolution of SARS-CoV-2 variants highlights the need for new therapies to prevent disease spread. SARS-CoV-2, like SARS-CoV-1, uses the human cell surface protein angiotensin-converting enzyme 2 (ACE2) as its native receptor. Here, we design and characterize a mutant ACE2 that enables rapid affinity purification of a dimeric protein by altering the active site to prevent autoproteolytic digestion of a C-terminal His
10
epitope tag. In cultured cells, mutant ACE2 competitiv… Show more
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