Development of a multiplex recombinase polymerase amplification coupled with lateral flow dipsticks for the simultaneous rapid detection of Salmonella spp., Salmonella Typhimurium and Salmonella Enteritidis
Zeqiang Zhan,
Shoukui He,
Yan Cui
et al.
Abstract:Salmonella spp. is one of the world-leading foodborne pathogens and its rapid detection is essential to ensure food safety. In this study, a visual method was established based on multiple recombinase polymerase amplification (RPA) coupled with lateral flow dipsticks (LFD) for the simultaneous detection of Salmonella spp., Salmonella Enteritidis (S. Enteritidis) and Salmonella Typhimurium (S. Typhimurium). The optimal volume and temperature for the multiplex RPA-LFD method were optimized as 25 μL and 38℃, resp… Show more
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